Bone marrow origin of decidual cell precursors in the pseudopregnant mouse uterus.

Abstract

Decidual cells are considered to be the endproduct of a hormonally induced transformation of endometrial stromal cells of the uterus. However, the source of these precursors remains unknown. This study of evaluated the possibility of their bone marrow origin by an examination of the H-2 phenotype of decidual cells in pseudopregnant bone marrow chimeras. These chimeras were produced by repopulating lethally irradiated CBA/J female (H-2k) mice with bone marrow from (CBA/J x C57BL/6J) F1 female (H-2kb) mice. Pseudopregnancy was produced with a hormonal regimen followed by an oil-induced decidual stimulus. Chimerism was evaluated radioautographically by an identification of the donor-specific Kb phenotype on cells with an immunolabeling technique with monospecific anti-H-2 serum followed by radioiodinated protein A. The extent of chimerism as indicated by the degree of Kb labeling on decidual cells as well as macrophages contained within the decidual nodules was quantitatively compared with that seen on splenic lymphocytes. Fair to good chimerism, as reflected by labeling for the donor-specific marker (Kb), was seen on splenic lymphocytes and macrophages within the decidual nodules in 6 out of 11 animals. A similar level of chimerism was detected on decidual cells in all but one of these six, in which case this was low. One animal showed low chimerism in the spleen but good chimerism on the decidual cells. The remaining four mice were nonchimeric for all three cell types. These results indicate that decidual cells and macrophages appearing within the decidual nodules of pseudopregnant mice are ultimate descendants of bone marrow cells.

Kinetics and regulation of 25-hydroxycholecalciferol 1alpha-hydroxylase from cells isolated from human term decidua

“…Using the mouse as a model, Kearns and Lala [27] reported results indicating that decidual cells as well as macrophages appearing within the decidual nodules of pseudopregnant animals are descendants of bone marrow cells. It may therefore well be that the cells used in this study pertain to the same lineage and that the enzyme is similar to that described for pulmonary alveolar macrophages in sarcoidosis 1281.…”

Expression of rat transforming growth factor alpha mRNA during development occurs predominantly in the maternal decidua.

“…Formation of the decidua (decidualization) is a poorly understood phenomenon involving cell proliferation and migration that is coordinated with cellular growth in the uterine epithelium (for a general description, see reference 9). It apparently results from the differentiation and proliferation of stem cells in the endometrial stroma that may have originally derived from the bone marrow (13). Once the uterus is sensitized by appropriate hormones, decidualization is then triggered, in part, by implantation of the embryo.…”

Haid daripada Perspektif Sains dan Maqasid Syariah

“…Cara alternatif adalah daripada darah haid yang mengandungi darah dan tisu yang luruh daripada endometrium yang mengandungi satu populasi sel yang heterogenus yang berupaya untuk tumbuh semula (Cui et al 2007). Sel rahim stromal mempunyai penanda multipoten sama yang biasa dijumpai di dalam sum-sum tulang (Kearns & Lala;Schwab & Gargett 2007;Taylor 2004).…”