Bone marrow mesenchymal stromal cells with support of bispecific antibody and ultrasound-mediated microbubbles prevent myocardial fibrosis via the signal transducer and activators of transcription signaling pathway

Deng, Wei; Chen, Qingwei; Li, Xingsheng; Líu, Hao; Niu, Siqiang; Zhou, Yue; Li, Guiqiong; Ke, Danxia; Mo, Xiangang

doi:10.3109/14653249.2010.542458

Cited by 6 publications

(5 citation statements)

References 43 publications

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“…Effective homing of membrane modified chondrogenic progenitors was observed to cartilage injury site in rabbit osteochondral explants (110). In another study, homing rate and repairing efficacy of MSCs improved using anti-CD29 and anti-myosin light chain bispecific antibodies in a mouse myocardial fibrosis model (111). Importantly, in this study Deng et al ( 111) also used highly focused ultrasound mediated stimulation of micro-bubbles, which significantly increases homing of MSCs to the mouse injured myocardium (112).…”

Section: Membrane and Cytoplasmic Msc Primingmentioning

confidence: 79%

Mesenchymal Stem Cell Functionalization for Enhanced Therapeutic Applications

Kouroupis

Sanjurjo‐Rodríguez

Jones

et al. 2019

Tissue Engineering Part B: Reviews

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To date, the therapeutic efficacy of human mesenchymal stem cells (hMSCs) has been investigated in various clinical trials with moderate or, in some cases, inconsistent results. The still elusive reproducibility relates, in part, with constitutive differences in the cell preparation, translated into variable "cell potencies." Other factors include poor cell homing and survival, and age-/disease-associated host tissue impairment. It is well accepted that within in vivo niches, MSCs exist as heterogeneous cell populations with different stemness propensities and supportive functions. Phenotype-based MSC purification of homogeneous subsets can result in cell populations with distinct biological functions. In addition, preclinical studies have shown that MSC functionalization in vitro, through cell priming, can boost their immunomodulatory, trophic, and reparative capacities in vivo. Therefore, in this review, we discuss how phenotype-based MSC purification and MSC priming technologies can contribute to an improved MSC-based product for safer and more effective therapeutic applications.

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Section: Membrane and Cytoplasmic Msc Primingmentioning

confidence: 79%

Mesenchymal Stem Cell Functionalization for Enhanced Therapeutic Applications

Kouroupis

Sanjurjo‐Rodríguez

Jones

et al. 2019

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

show abstract

“…Chemical modifications enable MSCs to provide several targeting moieties by covalent coupling methods, including ligands [ 95 ], peptides [ 92 ] and polymers [ 96 ]. Noncovalent modifications can improve cell-host properties without compromising the native properties of MSCs [ 97 ].…”

Section: Technologies For Engineering Mscs and Msc-exosmentioning

confidence: 99%

Research progress of engineered mesenchymal stem cells and their derived exosomes and their application in autoimmune/inflammatory diseases

Zhu

Yang

et al. 2023

Stem Cell Res Ther

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Autoimmune/inflammatory diseases affect many people and are an important cause of global incidence and mortality. Mesenchymal stem cells (MSCs) have low immunogenicity, immune regulation, multidifferentiation and other biological characteristics, play an important role in tissue repair and immune regulation and are widely used in the research and treatment of autoimmune/inflammatory diseases. In addition, MSCs can secrete extracellular vesicles with lipid bilayer structures under resting or activated conditions, including exosomes, microparticles and apoptotic bodies. Among them, exosomes, as the most important component of extracellular vesicles, can function as parent MSCs. Although MSCs and their exosomes have the characteristics of immune regulation and homing, engineering these cells or vesicles through various technical means, such as genetic engineering, surface modification and tissue engineering, can further improve their homing and other congenital characteristics, make them specifically target specific tissues or organs, and improve their therapeutic effect. This article reviews the advanced technology of engineering MSCs or MSC-derived exosomes and its application in some autoimmune/inflammatory diseases by searching the literature published in recent years at home and abroad.

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“…Similarly, this method was applied to repair the function of the infarcted heart by arming human HSCs with bispecific antibodies consisted of anti-human CD45 and anti-rat myosin light chain antibodies [131]. This technique was adapted by Deng et al to modify MSC with bispecific antibodies [132]. However, bispecific antibodies have not been widely used with MSC, likely due to the lack of a specific cell surface marker [133].…”

Section: Techniques For Engineering Mscmentioning

confidence: 99%

“…Employing molecular recognition techniques, such as surface-modified MSC with bispecific antibody [132] or peptide conjugation [134, 135], Deng et al improved the homing and therapeutic efficacy of MSC in a murine myocardial infarct model by combining the bispecific antibodies (anti-mouse CD29 and anti-myosin light chain antibody) with ultrasound-mediated MBs. The combination approach significantly increased the homing of MSC at the target site and reduced the fibrosis density compared with the unmodified MSC or MSC coated with bispecific antibodies only [132].…”

Section: Techniques For Engineering Mscmentioning

confidence: 99%

“…The combination approach significantly increased the homing of MSC at the target site and reduced the fibrosis density compared with the unmodified MSC or MSC coated with bispecific antibodies only [132]. This synergistic effect was due to the enhanced migration of the MSC, which changed their microenvironment by activating the STAT-mediated signaling pathway.…”

Section: Techniques For Engineering Mscmentioning

confidence: 99%

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Engineering mesenchymal stem cells for regenerative medicine and drug delivery

Park

Suryaprakash

Lao

et al. 2015

Methods

188

156

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Researchers have applied mesenchymal stem cells (MSC) to a variety of therapeutic scenarios by harnessing their multipotent, regenerative, and immunosuppressive properties with tropisms toward inflamed, hypoxic, and cancerous sites. Although MSC-based therapies have been shown to be safe and effective to a certain degree, the efficacy remains low in most cases when MSC are applied alone. To enhance their therapeutic efficacy, researchers have equipped MSC with targeted delivery functions using genetic engineering, therapeutic agent incorporation, and cell surface modification. MSC can be genetically modified virally or non-virally to overexpress therapeutic proteins that complement their innate properties. MSC can also be primed with non-peptidic drugs or magnetic nanoparticles for enhanced efficacy and externally regulated targeting, respectively. Furthermore, MSC can be functionalized with targeting moieties to augment their homing toward therapeutic sites using enzymatic modification, chemical conjugation, or non-covalent interactions. These engineering techniques are still works in progress, requiring optimization to improve the therapeutic efficacy and targeting effectiveness while minimizing any loss of MSC function. In this review, we will highlight the advanced techniques of engineering MSC, describe their promise and the challenges of translation into clinical settings, and suggest future perspectives on realizing their full potential for MSC-based therapy.

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Bone marrow mesenchymal stromal cells with support of bispecific antibody and ultrasound-mediated microbubbles prevent myocardial fibrosis via the signal transducer and activators of transcription signaling pathway

Cited by 6 publications

References 43 publications

Mesenchymal Stem Cell Functionalization for Enhanced Therapeutic Applications

Mesenchymal Stem Cell Functionalization for Enhanced Therapeutic Applications

Research progress of engineered mesenchymal stem cells and their derived exosomes and their application in autoimmune/inflammatory diseases

Engineering mesenchymal stem cells for regenerative medicine and drug delivery

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