Bone Marrow Mesenchymal Stem Cell Transplantation for Improving Nerve Regeneration

Oliveira, Jorge de; Mostacada, Klauss; Lima, Silmara de; Martínez, Ana Maria Blanco

doi:10.1016/b978-0-12-410499-0.00003-4

Cited by 33 publications

(19 citation statements)

References 49 publications

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“…It is known that, differently to the Central Nervous System (CNS), the Peripheral Nervous System (PNS) is able to regenerate spontaneously in response to injury although the regeneration process is closely related to the severity of the damage. After a nerve injury, several mechanisms occur at the injury site almost immediately including morphologic and metabolic changes [15][16][17].…”

Section: The Biological Mechanisms Of Peripheral Nerve Regenerationmentioning

confidence: 99%

Strategies to improve nerve regeneration after radical prostatectomy: a narrative review

Geuna¹,

Muratori²,

Fregnan³

et al. 2018

Minerva Urol Nefrol

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Peripheral nerves are complex organs that spread throughout the entire human body. They are frequently affected by lesions not only as a result of trauma but also following radical tumor resection. In fact, despite the advancement in surgical techniques, such as nervesparing robot assisted radical prostatectomy, some degree of nerve injury may occur resulting in erectile dysfunction with significant impairment of the quality of life.The aim of this review is to provide an overview on the mechanisms of the regeneration of injured peripheral nerves and to describe the potential strategies to improve the regeneration process and the functional recovery. Yet, the recent advances in bioengineering strategies to promote nerve regeneration in the urological field are outlined with a view on the possible future regenerative therapies which might ameliorate the functional outcome after radical prostatectomy.

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Section: The Biological Mechanisms Of Peripheral Nerve Regenerationmentioning

confidence: 99%

Strategies to improve nerve regeneration after radical prostatectomy: a narrative review

Geuna¹,

Muratori²,

Fregnan³

et al. 2018

Minerva Urol Nefrol

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“…SCs are so important to nerve regeneration that further alternatives for them have been explored by using differentiated stem cells (ADSC or iPSC) to SC-like cells [14],[20],[53],[54] or BMSC to effect nerve regeneration, either by direct and/or indirect paracrine signaling to express glial markers. [55],[56] SCs can be induced from ADSCs cells by addition of glial growth factor and co-culture with SCs. A key factor when working with cell differentiation and proliferation is cell density, since it has been reported that higher cell density equals an increase of cell-cell interactions, which is also related to the influence of glial growth factor.…”

Section: Selection Of Cellsmentioning

confidence: 99%

Polymeric scaffolds for three-dimensional culture of nerve cells: a model of peripheral nerve regeneration

et al. 2017

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Understanding peripheral nerve repair requires the evaluation of 3D structures that serve as platforms for 3D cell culture. Multiple platforms for 3D cell culture have been developed, mimicking peripheral nerve growth and function, in order to study tissue repair or diseases. To recreate an appropriate 3D environment for peripheral nerve cells, key factors are to be considered including: selection of cells, polymeric biomaterials to be used, and fabrication techniques to shape and form the 3D scaffolds for cellular culture. This review focuses on polymeric 3D platforms used for the development of 3D peripheral nerve cell cultures.

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“…Therefore, mesenchymal stem cells (MSCs) have been addressed as an easy to access and potentially unlimited cell source for tissue-engineered nerve grafts. 9 In this study, we performed stem cell engineering by nonviral genetic modification of bone marrow-derived mesenchymal stem cells (BMSCs), resulting in overexpression of selected NTFs. Another option to ensure availability of NTFs within an artifical nerve graft is the conjugation of the proteins to nanoparticles and their delivery within a hydrogel matrix for axonal regeneration.…”

Section: Introductionmentioning

confidence: 99%

Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials

Morano¹,

Wróbel²,

Fregnan³

et al. 2014

IJN

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Purpose Innovative nerve conduits for peripheral nerve reconstruction are needed in order to specifically support peripheral nerve regeneration (PNR) whenever nerve autotransplantation is not an option. Specific support of PNR could be achieved by neurotrophic factor delivery within the nerve conduits via nanotechnology or stem cell engineering and transplantation. Methods Here, we comparatively investigated the bioactivity of selected neurotrophic factors conjugated to iron oxide nanoparticles (np-NTFs) and of bone marrow-derived stem cells genetically engineered to overexpress those neurotrophic factors (NTF-BMSCs). The neurite outgrowth inductive activity was monitored in culture systems of adult and neonatal rat sensory dorsal root ganglion neurons as well as in the cell line from rat pheochromocytoma (PC-12) cell sympathetic culture model system. Results We demonstrate that np-NTFs reliably support numeric neurite outgrowth in all utilized culture models. In some aspects, especially with regard to their long-term bioactivity, np-NTFs are even superior to free NTFs. Engineered NTF-BMSCs proved to be less effective in induction of sensory neurite outgrowth but demonstrated an increased bioactivity in the PC-12 cell culture system. In contrast, primary nontransfected BMSCs were as effective as np-NTFs in sensory neurite induction and demonstrated an impairment of neuronal differentiation in the PC-12 cell system. Conclusion Our results evidence that nanotechnology as used in our setup is superior over stem cell engineering when it comes to in vitro models for PNR. Furthermore, np-NTFs can easily be suspended in regenerative hydrogel matrix and could be delivered that way to nerve conduits for future in vivo studies and medical application.

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Bone Marrow Mesenchymal Stem Cell Transplantation for Improving Nerve Regeneration

Cited by 33 publications

References 49 publications

Strategies to improve nerve regeneration after radical prostatectomy: a narrative review

Strategies to improve nerve regeneration after radical prostatectomy: a narrative review

Polymeric scaffolds for three-dimensional culture of nerve cells: a model of peripheral nerve regeneration

Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials

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Bone Marrow Mesenchymal Stem Cell Transplantation for Improving Nerve Regeneration

Cited by 33 publications

References 49 publications

Strategies to improve nerve regeneration after radical prostatectomy: a narrative review

Strategies to improve nerve regeneration after radical prostatectomy: a narrative review

Polymeric scaffolds for three-dimensional culture of nerve cells: a model of peripheral nerve regeneration

Nanotechnology versus stem cell engineering: in&nbsp;vitro comparison of neurite inductive potentials

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Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials