Bone marrow-derived stem cells for ischemic hearts

Kocher, Alfred

doi:10.1007/bf03040281

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…To date, numerous experimental models and clinical trials have focused on evaluating the angiogenic potential of various subtypes of blood-derived mononuclear cells, endothelial progenitor cells, [1][2][3] and bone marrow stem/stromal cells. [4][5][6][7][8][9][10][11][12][13] It has been shown that endothelial colony forming cells, 14 a subtype of blood-derived endothelial progenitor cells, form functional vessels in vivo when implanted in mice 15 ; however, the newly formed vessels were limited in frequency and size. 16 This finding agrees with prior observations demonstrating that mature endothelial cells (ECs) are able to establish only single-layer narrow-caliber vessels, 17 whereas genetic modification of ECs with bcl-2, which repressed EC apoptosis, led to formation of large-caliber, functional vessels with thick walls.…”

Section: Introductionmentioning

confidence: 99%

Adipose Tissue Progenitor Cells Directly Interact with Endothelial Cells to Induce Vascular Network Formation

Merfeld-Clauss

Gollahalli

March

et al. 2010

Tissue Engineering Part A

165

166

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Adipose stromal cells (ASCs) express markers and functional properties of pericytes in vitro and, in combination with endothelial cells (ECs), are able to establish multilayer functional vessels in vivo. However, the factors that coordinate EC-ASC communications to promote migration of these cells toward one another, and their heterotypic assembly into vascular structures are not well defined. To understand the mechanisms of EC-ASC interaction, we developed an in vitro model of coculturing ECs with ASCs in a system containing serum but no additional exogenous cytokines or extracellular matrix (ECM) proteins. We demonstrated that ASCs have a profound potential to stimulate morphogenesis of ECs into branching networks of cord structures. The vascular networks developed in 6 days and were stable for at least 3 weeks. This process was associated with an increase in ECM protein production by ASCs and ECs, alpha-smooth muscle actin expression by ASCs, and increased CD31/platelet endothelial cell adhesion molecule-1 (PECAM-1) surface presentation by ECs. The vascular network formation (VNF) was dependent on matrix metalloproteinase activity and cell communications through vascular endothelial growth factor, hepatocyte growth factor, and platelet-derived growth factor-BB pathways. ASCs exhibited significantly higher potential to stimulate VNF than smooth muscle cells and fibroblasts. Media conditioned by ASCs promoted VNF by ECs cultured on smooth muscle cells and fibroblasts, but could not replace the presence of ASCs in coculture. The presence of ASCs in EC-fibroblast cocultures in a low fraction efficiently stimulated VNF. These findings demonstrate that the vasculogenesis-promoting potential of ASCs depends on interaction with ECs involving contact and likely bi-directional interaction, resulting in modulated secretion of cytokines and ECM proteins.

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Section: Introductionmentioning

confidence: 99%

Adipose Tissue Progenitor Cells Directly Interact with Endothelial Cells to Induce Vascular Network Formation

Merfeld-Clauss

Gollahalli

March

et al. 2010

Tissue Engineering Part A

165

166

View full text Add to dashboard Cite

show abstract

“…Initial studies involved skeletal myoblasts [1][2][3], bone marrow-derived mesenchymal stromal cells [4][5][6][7][8][9][10][11][12][13][14][15], and various subtypes of blood-derived endothelial progenitor cells (EPCs) [15][16][17][18][19][20]. There has been particular interest in studies of cell therapy for diseases of the cardiovascular system.…”

Section: Introductionmentioning

confidence: 99%