Bone marrow-derived mononuclear cell therapy attenuates silica-induced lung fibrosis

Abstract

This study tests the hypothesis that bone marrow-derived mononuclear cell (BMDMC) therapy may reduce lung inflammation and fibrosis leading to an improvement in respiratory mechanics in a murine model of silicosis.52 female C57BL/6 mice were randomly assigned into four groups. In the silica group (SIL), silica suspension (20 mg/50 mL in saline) was intratracheally instilled. In the control animals, 50 mL saline was administered intratracheally. At 1 h, the control and SIL groups were further randomised, receiving BMDMC (2610 6 In the SIL-cell group, the fraction area of granuloma, the number of macrophages and the collagen fibre content were reduced, yielding improved lung mechanics. The presence of male donor cells in lung tissue was not confirmed using detection of Y chromosome DNA. Nevertheless, caspase-3, IL-1b, IL-1a, IL-1RN and TGF-b mRNA expression diminished after cell therapy.In conclusion, BMDMC acted on inflammatory and fibrogenic processes improving lung function through paracrine effects.

Rescue of murine silica-induced lung injury and fibrosis by human embryonic stem cells

“…Bone marrow-derived cells, once instilled into silicainjured mice, allowed a transient beneficial effect until 2 months after cell administration. Similar paracrine effects were described using bone marrow-derived mononuclear cells, for which no engraftment was reported despite an evident improvement of lung function [35].…”

“…Other studies have been carried out using stem cell transplantation (either mesenchymal cells or cells derived from fetal membranes of human term placenta) in animal models in which lung fibrosis has been induced by bleomycin and also by silica [26,[33][34][35][36]. These cells were found to express alveolar epithelium markers after injection into mice and to exert significant antiinflammatory and anti-fibrotic effects in the bleomycin as well as in the silica models of fibrosis.…”

Repeated Administration of Bone Marrow-Derived Cells Prevents Disease Progression in Experimental Silicosis

“…Silicosis is an occupational disease that causes respiratory failure due to reduction in the area of gas exchange and deterioration in lung function [1,2,3]. To date, there has been no effective treatment to minimize the development of this disease.…”

“…In experimental models of lung fibrosis, bone marrow-derived cells (BMDCs) were able to repair damaged tissue by modulating the inflammatory process and replacing damaged cells [3,5,6,7]. …”

Impact of Mesenchymal Stem Cells on Lipopolysaccharide-Induced Apoptosis in Acute Lung Injury Rat Model

“…The second group received Escherichia coli-LPS (20µg/rat/day) for two consecutive days intranasal (Wagner et al, 2003) with modification. The third group received (1x10 6 ) bone marrow derived mesenchymal stem cells in 500 µl PBS/rat via intraperitoneal injection (Maron-Gutierrez et al, 2011). The fourth group received Dex (2mg/kg, i.p; Amriya Pharm, Egypt) once (Lin et al, 2006;Lv, 2016).…”