Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression – implications for atherosclerosis research

Bisgaard, Line Stattau; Mogensen, Christina; Rosendahl, Alexander; Cucak, Helena; Nielsen, Lars Bo; Rasmussen, Salka E.; Pedersen, T.

doi:10.1038/srep35234

Cited by 87 publications

(64 citation statements)

References 31 publications

(49 reference statements)

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“…In contrast to our findings, previous studies have failed to show increase in glucose uptake in resident peritoneal macrophages activated with IFNγ plus TNF (55). This discrepancy can be explained by functional differences between macrophage sources including the magnitude of their responses to pro-inflammatory and anti-inflammatory stimuli (56,57). Macrophages can also be activated by other stimuli including pathogen-associated molecular patterns (PAMPs) such as LPS.…”

Section: Discussioncontrasting

confidence: 99%

Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis

et al. 2020

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Altered lipid metabolism in macrophages is associated with various important inflammatory conditions. Although lipid metabolism is an important target for therapeutic intervention, the metabolic requirement involved in lipid accumulation during pro-inflammatory activation of macrophages remains incompletely characterized. We show here that macrophage activation with IFNγ results in increased aerobic glycolysis, iNOS-dependent inhibition of respiration, and accumulation of triacylglycerol. Surprisingly, metabolite tracing with 13 C-labeled glucose revealed that the glucose contributed to the glycerol groups in triacylglycerol (TAG), rather than to de novo synthesis of fatty acids. This is in stark contrast to the otherwise similar metabolism of cancer cells, and previous results obtained in activated macrophages and dendritic cells. Our results establish a novel metabolic pathway whereby glucose provides glycerol to the headgroup of TAG during classical macrophage activation.

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Section: Discussioncontrasting

confidence: 99%

Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis

et al. 2020

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“…Measured differences of CD16 + monocytes in human CAD (Ozaki et al., ) and of M2 macrophages in mouse atherosclerosis (our laboratory; also Bisgaard et al., ) support our cohort as being ≥80% powered to reject null hypotheses correctly, with α = 0.05, for both these measures. For the baseline characteristics of the three groups by CAD severity, one‐way ANOVA was used to compare continuous variables, and post hoc testing was performed using Student's unpaired t tests.…”

Section: Methodssupporting

confidence: 76%

Monocyte and macrophage subtypes as paired cell biomarkers for coronary artery disease

Arnold

Blair

Paul

et al. 2019

Experimental Physiology

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New Findings What is the central question of this study?Are circulating monocyte markers correlated with their derived macrophage polarization patterns and coronary artery disease severity? What is the main finding and its importance?There was an inverse relationship between circulating CD16+ monocytes (high) and M2 macrophages (low) that marked coronary disease severity, and the differences in polarization of macrophages were seen despite a week of cell culture ex vivo. This study highlights the importance, and potential prognostic implications, of circulating monocyte and descendant macrophage phenotypes in coronary artery disease. Abstract Monocytes and macrophages are central to atherosclerosis, but how they combine to mark progression of human coronary artery disease (CAD) is unclear. We tested whether patients’ monocyte subtypes paired with their derived macrophage profiles were correlated with extent of CAD. Peripheral blood was collected from 40 patients undergoing cardiac catheterization, and patients were categorized as having no significant CAD, single vessel disease or multivessel disease according to the number of affected coronary arteries. Mononuclear cells were measured for the monocyte markers CD14 and CD16 by flow cytometry, and separate monocytes were cultured into macrophages over 7 days and measured for the polarization markers CD86 and CD206. At baseline, patients with a greater CAD burden were older, with higher rates of statin, β‐blocker and antiplatelet drug use, whereas other characteristics were similar across the spectrum of coronary disease. CD16+ (both intermediate and non‐classical) monocytes were elevated in patients with single vessel and multivessel disease compared with those without significant CAD (P < 0.05), whereas regulatory M2 macrophages (CD206+) were decreased in patients with single vessel and multivessel disease (P < 0.001). An inverse relationship between paired CD16+ monocytes and M2 macrophages marked CAD severity. On multivariable linear regression, CAD severity was associated, along with age and traditional cardiovascular risk factors, with CD16+ monocytes (directly) and M2 macrophages (inversely). Circulating monocytes may influence downstream polarization of lesional macrophages, and these measures of monocyte and macrophage subtypes hold potential as biomarkers in CAD.

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“…Primary cultures of BMDMs from indicated mice were prepared as previously described (43). Briefly, bone marrow cells were harvested from the femurs and tibias of mice.…”

Section: Bmdm Preparationmentioning

confidence: 99%

“…1D). Because PMs have been reported to be activated macrophages (43,53), we examined the surface activation markers and CD23 expression in PMs and BMDMs. We found that PMs expressed higher levels of CD86 (M1 phase macrophage marker) and CD23 ( Supplemental Fig.…”

Section: Cd23 Is Necessary For C Albicans-induced Nf-kb Activation Imentioning

confidence: 99%

C-Type Lectin Receptor CD23 Is Required for Host Defense against Candida albicans and Aspergillus fumigatus Infection

Guo

Chang

Cheng

et al. 2018

The Journal of Immunology

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Infection by invasive fungi, such as ,, and , is one of the leading death causes for the increasing population of immunocompromised and immunodeficient patients. Several C-type lectin receptors (CLRs), including Dectin-1, -2, and -3 and Mincle can recognize fungal surface components and initiate the host antifungal immune responses. Nevertheless, it remains to be determined whether other CLRs are involved in antifungal immunity. Our recent study suggests that CD23 (CLEC4J), a CLR and also a well-known B cell surface marker, may function to sense components in antifungal immunity. However, it is not clear how CD23 functions as a fungal pattern recognition receptor and whether the antifungal role of CD23 is specific to or not. In this study, we show that CD23 can recognize both α-mannan and β-glucan from the cell wall of or but cannot recognize glucuronoxylomannan from Through forming a complex with FcRγ, CD23 can induce NF-κB activation. Consistently, CD23-deficient mice were highly susceptible to and but not to infection. The expression of CD23 in activated macrophages is critical for the activation of NF-κB. CD23 deficiency results in impaired expression of NF-κB-dependent genes, especially, which induces NO production to suppress fungal infection. Together, our studies reveal the CD23-induced signaling pathways and their roles in antifungal immunity, specifically for and, which provides the molecular basis for designing potential therapeutic agents against fungal infection.

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Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression – implications for atherosclerosis research

Cited by 87 publications

References 31 publications

Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis

Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis

Monocyte and macrophage subtypes as paired cell biomarkers for coronary artery disease

C-Type Lectin Receptor CD23 Is Required for Host Defense against Candida albicans and Aspergillus fumigatus Infection

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