Bone marrow aspirate combined with low-level laser therapy: A new therapeutic approach to enhance bone healing

Nagata, Maria José Hitomi; Santinoni, Carolina dos Santos; Pola, Natália Marcumini; Campos, Natália de; Messora, Michel Reis; Bomfim, Suely Regina Mogami; Ervolino, Edílson; Fucini, Stephen E.; Faleiros, Paula Lazilha; Garcia, Valdir Gouveia; Bosco, Álvaro Francisco

doi:10.1016/j.jphotobiol.2013.01.013

Cited by 57 publications

(64 citation statements)

References 56 publications

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“…Histomorphometric analysis revealed significant differences in favor of Group AFGL when compared to Group AFG at all postoperative periods, with a thinner interface. The neovascularization and thermic and biological stimuli provided by LLLT can lead to better graft integration [32,[35][36][37][38].…”

Section: Discussionmentioning

confidence: 99%

Effects of low-level laser therapy on autogenous bone graft stabilized with a new heterologous fibrin sealant

Gonçalves

Buchaim

Bueno³

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Autogenous bone grafts are used to repair bone defects, and the stabilization is needed for bone regeneration. Laser photobiomodulation is a modality of treatment in clinical practice for tissue regeneration, and it has therapeutic effects as an anti-inflammatory, analgesic and modulating cellular activity. The aim of the present study was to evaluate the effects of low-level laser therapy (LLLT) on an autogenous bone graft integration process stabilized with a new heterologous fibrin sealant. Forty rats were divided into two groups: Autogenous Fibrin Graft (AFG, n=20), in which a 5mm dome osteotomy was conducted in the right parietal bone and the graft was adhered to the left side using fibrin sealant; and Autogenous Fibrin Graft Laser (AFGL, n=20), which was subjected to the same procedures as AFG with the addition of LLLT. The treatment was performed immediately following surgery and then three times a week until euthanasia, using an 830nm laser (30mW, 6J/cm(2), 0.116cm(2), 258.6mW/cm(2), 2.9J). Five animals from each group were euthanized at 10, 20, 30 and 40days postoperative, and the samples were submitted to histomorphological and histomorphometric analysis. Partial bone regeneration occurred, with new bone tissue integrating the graft to the recipient bed and small areas of connective tissue. Comparative analysis of the groups at the same intervals revealed minor interfaces in group AFGL, with statistically significant differences (p<0.05) at all of the analyzed intervals (10days p=0.0087, 20days p=0.0012, 30days p<0.0001, 40days p=0.0142). In conclusion, low-level laser therapy stimulated bone regeneration and accelerated the process of integration of autogenous bone grafts.

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Section: Discussionmentioning

confidence: 99%

Effects of low-level laser therapy on autogenous bone graft stabilized with a new heterologous fibrin sealant

Gonçalves

Buchaim

Bueno³

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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“…The combination of bone marrow aspirate/LLL yielded significantly greater bone formation in surgically created critical-size defects in rat calvaria[11]. LLL was applied as an adjunct therapy for MSCs transplantation on the functional recovery of crushed sciatic nerve in rats[12].…”

Section: Introductionmentioning

confidence: 99%

Low level laser (LLL) attenuate LPS-induced inflammatory responses in mesenchymal stem cells via the suppression of NF-κB signaling pathway in vitro

et al. 2017

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BackgroundConsidering promising results in animal models and patients, therapeutic use of MSCs for immune disease is likely to undergo continued evaluation. Low-lever laser (LLL) has been widely applied to retard the inflammatory reaction. LLL treatment can potentially be applied in anti-inflammatory therapy followed by stem cell therapy.Aim of the studyThe purpose of this study was to investigate the effect of LLL (660 nm) on the inflammatory reaction induced by LPS in human adipose derived mesenchymal stem cells (hADSCs) and pertinent mechanism.Materials and methodsAnti-inflammatory activity of LLL was investigated by LPS-induced mesenchymal stem cells. The production and expression of pro-inflammatory cytokines were evaluated by ELISA kits and RT-qPCR. Nuclear translocation of NF-κB was indicated by immunofluorescent staining. Phosphorylation status of NF-κB p65 and IκBα were illustrated by western blot assay. ROS generation was measured with CM-H2DCFDA, and NO secretion was determined by DAF-FM. We studied surface expression of lymphocyte activation markers when Purified peripheral blood mononuclear cell (PBMC) were activated by phytohaemagglutinin (PHA) in the presence of 3 types of treated MSCs.ResultsLLL reduced the secretion of IL-1β, IL-6, IL8, ROS and NO in LPS treated MSCs. Immunofluorescent assay demonstrated the nuclear translocation decrease of NF-κB in LLL treated LPS induced MSCs. Western blot analysis also suggested that LLL suppressed NF-κB activation via regulating the phosphorylation of p65 and IκBα. MSC significantly reduced the expression of activation markers CD25 and CD69 on PHA-stimulated lymphocytes.ConclusionThe results indicate that LLL suppressed the activation of NF-κB signaling pathway in LPS treated MSCs through inhibiting phosphorylation of p65 and IκBα, which results in good anti-inflammatory effect. In addition, LLL attenuated activation-associated markers CD25 and CD69 in co-cultures of PBMC and 3 types of treated MSCs.

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“…On the other hand, Mouraret S et al, when comparing ultrasonic surgical instruments and a fissure carbide bur, used reported that no obvious differences were found in the extent or duration of postoperative inflammation 25) . PCNA-positive cells are used as an indicator of cell proliferation 26,27) and OC is used as an indicator of osteoblast activity in the later stages of osteogenesis 26) . In this study, we compared the bone healing process using these two assessment methods, and no difference was found in the healing process.…”

Section: Healing Process After Bone Cuttingmentioning

confidence: 99%

Comparison of Surface Morphology and Healing in Rat Calvaria Bone Defects between Ultrasonic Surgical Method and Rotary Cutting Method

Matsuda

Sasaki

Takanashi

et al. 2015

J. Hard Tissue Biology.

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The aim of this study was to investigate the influence of ultrasonic surgical method (piezoelectric surgery) on invasions of bone structure in early stages and the subsequent healing process of bone defects of rat calvaria as compared with that of rotary cutting using an appropriate rotation speed. Thirteen-week-old male Sprague-Dawley rats were used in this study. Bone defects were prepared in rat calvaria using either an ultrasonic surgical instrument with an ultrasonic insert tip (Ui) or a handpiece with a round bur (Rb) as a rotary cutting instrument (control). Morphological evaluations of the cutting surfaces in the bone defects were assessed by scanning electron microscope (SEM) and confocal laser microscope (CLM) observation. The degree of invasions of the bone structure in the early stages after bone defects were made was assessed by the ratio of empty osteocyte lacunae relative to the total number of osteocytes. The process of bone healing was analyzed by histological observation, immunohistochemical staining with proliferating cell nuclear antigen (PCNA) and osteocalcin (OC), and ratio of PCNA-positive cells. Many fine, parallel streaky cutting traces were observed in the Ui groove surfaces, whereas the Rb groove surfaces were flat and no parallel cutting traces were observed. The arithmetic average roughness of the Ui group (10.06 ± 1.96 µm) was significantly higher than that of the Rb group (2.52 ± 0.16 µm) (p < 0.05). During the entire observation period, no differences in histological observation and immunohistochemical staining were observed between the Ui and Rb groups. No significant differences were recognized in the ratio of empty lacunae and PCNA-positive cells between the Ui and Rb groups. In the present study, there were no appreciable differences in the invasions of bone structure in the early stages and the subsequent healing process of the bone defects of rat calvaria between ultrasonic surgical method and rotary cutting method.

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Bone marrow aspirate combined with low-level laser therapy: A new therapeutic approach to enhance bone healing

Cited by 57 publications

References 56 publications

Effects of low-level laser therapy on autogenous bone graft stabilized with a new heterologous fibrin sealant

Effects of low-level laser therapy on autogenous bone graft stabilized with a new heterologous fibrin sealant

Low level laser (LLL) attenuate LPS-induced inflammatory responses in mesenchymal stem cells via the suppression of NF-κB signaling pathway in vitro

Comparison of Surface Morphology and Healing in Rat Calvaria Bone Defects between Ultrasonic Surgical Method and Rotary Cutting Method

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