Comparison of Surface Morphology and Healing in Rat Calvaria Bone Defects between Ultrasonic Surgical Method and Rotary Cutting Method

Abstract

The aim of this study was to investigate the influence of ultrasonic surgical method (piezoelectric surgery) on invasions of bone structure in early stages and the subsequent healing process of bone defects of rat calvaria as compared with that of rotary cutting using an appropriate rotation speed. Thirteen-week-old male Sprague-Dawley rats were used in this study. Bone defects were prepared in rat calvaria using either an ultrasonic surgical instrument with an ultrasonic insert tip (Ui) or a handpiece with a round bur (Rb) as a rotary cutting instrument (control). Morphological evaluations of the cutting surfaces in the bone defects were assessed by scanning electron microscope (SEM) and confocal laser microscope (CLM) observation. The degree of invasions of the bone structure in the early stages after bone defects were made was assessed by the ratio of empty osteocyte lacunae relative to the total number of osteocytes. The process of bone healing was analyzed by histological observation, immunohistochemical staining with proliferating cell nuclear antigen (PCNA) and osteocalcin (OC), and ratio of PCNA-positive cells. Many fine, parallel streaky cutting traces were observed in the Ui groove surfaces, whereas the Rb groove surfaces were flat and no parallel cutting traces were observed. The arithmetic average roughness of the Ui group (10.06 ± 1.96 µm) was significantly higher than that of the Rb group (2.52 ± 0.16 µm) (p < 0.05). During the entire observation period, no differences in histological observation and immunohistochemical staining were observed between the Ui and Rb groups. No significant differences were recognized in the ratio of empty lacunae and PCNA-positive cells between the Ui and Rb groups. In the present study, there were no appreciable differences in the invasions of bone structure in the early stages and the subsequent healing process of the bone defects of rat calvaria between ultrasonic surgical method and rotary cutting method.

Prognostic factors for maxillary sinus mucosal thickening following Le Fort I osteotomy: a retrospective analysis

“…In the evaluation of factors related to mucosal thickening, the following eight factors were examined: sex, age (mean age at the time of 1 month after surgery), diagnosis (skeletal diagnosis of the maxilla), operating time (total operating time and operating time at the end of Le Fort I osteotomy), amount of postoperative bleeding (total bleeding and bleeding at the end of Le Fort I osteotomy), with/without bone graft in Le Fort I osteotomy, with/without multisegmental osteotomy in Le Fort I osteotomy, and with/without macrolide therapy after surgery. The ultrasonic surgical method (piezoelectric surgery) was mainly used for the separation of the maxilla [4, 5]. In addition, macrolide therapy was implemented in accordance with previously reported research and guidelines [6–8].…”

Cutting bone with drills, burs, lasers and piezotomes: A comprehensive systematic review and recommendations for the clinician

“…Burs/drills vs piezotomes: With moderate to strong evidence (EL: 1,3; Table 1) and consecutive substantial clinical impact piezotomes seem to provide an improved bonehealing, proved on molecular-biologic, micromorphologic and histologic level in vivo, when compared to rotary instruments. Furthermore, a superior soft-tissue-preservation is reported compared to drills and burs [70][71][72][73][74][75][76][77][78][79][80][81][82][83][84][85][86].…”

Histological Evaluation of Alveolar Bone Ridge for Dental Implant Placement Using a Nondecalcified Frozen Section Technique

“…However, the majority of these have used the decalcified sectioning method 17,18) . To date, reports of studies such as the present, with biological analysis of bone quality at jawbone site, have been non-existent both in Japan and overseas due to 63 This study used the nondecalcified frozen section technique to evaluate the bone quality of harvested bone by site.…”