Bone Grafts and Substitutes

Cutter, Calvin; Mehrara, B J

doi:10.1615/jlongtermeffmedimplants.v16.i3.50

Cited by 23 publications

(24 citation statements)

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“…However, bone substitute and grafting biomaterials are becoming increasingly important for all aspects of surgery and dentistry [1][2][3][4]. Within the broad range of biomaterials used in craniofacial bone surgery [5], calcium phosphate-based bioceramics, e.g.…”

Section: Introductionmentioning

confidence: 99%

A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

Götz

Lenz²,

Reichert³

et al. 2011

Folia Histochem Cytobiol.

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Abstract:To test the probable osteoinductive properties of NanoBone®, a new highly non-sintered porous nano-crystalline hydroxylapatite bone substitute embedded into a silica gel matrix, granules were implanted subcutaneously and intramuscularly into the back region of 18 mini pigs. After periods of 5 and 10 weeks as well as 4 and 8 months, implantation sites were investigated using histological and histomorphometric procedures. Signs of early osteogenesis could already be detected after 5 weeks. The later periods were characterized by increasing membranous osteogenesis in and around the granules leading to the formation of bone-like structures showing periosteal and tendon-like structures with bone marrow and focal chondrogenesis. Bone formation was better in the subcutaneous than in the intramuscular implantation sites. This ectopic osteogenesis is discussed with regard to the nanoporosity and microporosity of the material, physico-chemical interactions at its surface, the differentiation of osteoblasts, the role of angiogenesis and the probable involvement of growth factors. The results of this preliminary study indicate that this biomaterial has osteoinductive potential and induces the formation of bone structures, mainly in subcutaneous adipose tissue in the pig.

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Section: Introductionmentioning

confidence: 99%

A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

Götz

Lenz²,

Reichert³

et al. 2011

Folia Histochem Cytobiol.

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“…1 Regenerative medicine offers an exciting approach for bone repair and regeneration, which involves the synergistic combination of stem cells with suitable scaffolds. [2][3][4] Stem cells have the ability to differentiate into one or more tissue types.…”

mentioning

confidence: 99%

Human Embryonic Stem Cell-Derived Mesenchymal Stem Cell Seeding on Calcium Phosphate Cement-Chitosan-RGD Scaffold for Bone Repair

Chen

Zhou

Weir

et al. 2013

Tissue Engineering Part A

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Calcium phosphate cement (CPC) has in situ-setting ability and excellent osteoconductivity. Human embryonic stem cells (hESCs) are exciting for regenerative medicine due to their strong proliferative ability and multilineage differentiation capability. However, there has been no report on hESC seeding with CPC. The objectives of this study were to obtain hESC-derived mesenchymal stem cells (hESCd-MSCs), and to investigate hESCd-MSC proliferation and osteogenic differentiation on novel CPC with chitosan immobilized with RGD (CPC-chitosan-RGD). RGD was covalently bonded with chitosan, which was then incorporated into CPC. The CPC-chitosan-RGD scaffold had higher strength and toughness than CPC-chitosan control without RGD ( p < 0.05). hESCs were cultured to form embryoid bodies (EBs), and the MSCs were then migrated out of the EBs. Flow cytometry indicated that the hESCd-MSCs expressed typical surface antigen profile of MSCs. hESCd-MSCs had good viability when seeded on CPC scaffolds. The percentage of live cells and the cell density were significantly higher on CPC-chitosan-RGD than CPC-chitosan control. Scanning electron microscope examination showed hESCd-MSCs with a healthy spreading morphology adherent to CPC. hESCd-MSCs expressed high levels of osteogenic markers, including alkaline phosphatase, osteocalcin, collagen I, and Runx2. The mineral synthesis by the hESCd-MSCs on the CPC-chitosan-RGD scaffold was twice that for CPC-chitosan control. In conclusion, hESCs were successfully seeded on CPC scaffolds for bone tissue engineering. The hESCd-MSCs had good viability and osteogenic differentiation on the novel CPC-chitosan-RGD scaffold. RGD incorporation improved the strength and toughness of CPC, and greatly enhanced the hESCd-MSC attachment, proliferation, and bone mineral synthesis. Therefore, the hESCd-MSC-seeded CPC-chitosan-RGD construct is promising to improve bone regeneration in orthopedic and craniofacial applications.

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“…In this study, low crystalline microporous calcite was made by exposing calcium hydroxide compact to carbone dioxide. Therefore, NaH 2 PO 4 aqueous solution can penetrate in to the interior of the calcite Journal of the Ceramic Society of Japan 118 [5] 341-344 2010 block even at 60°C and dissolution-precipitation reaction took place. When marine coral, whose dominant inorganic component is aragonite; the other modification of CaCO 3 , was exposed to (NH 4 ) 2 HPO 4 , compositional transformation was observed only on the surface of marine coral.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of low crystalline B-type carbonate apatite block from low crystalline calcite block

Ishikawa

Matsuya

Lin

et al. 2010

J. Ceram. Soc. Japan

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Low crystalline B-type carbonate apatite (CO 3 Ap) block was fabricated by compositional transformation based on dissolution precipitation reaction using microporous low crystalline calcite block as a precursor. In other words, microporous low crystalline calcite block prepared by carbonation of Ca(OH) 2 compact was phosphoridated by exposing to 1 mol·dm ¹3 Na 2 HPO 4 solutions at 60°C for 14 days. XRD, FT-IR and SEM analysis showed that calcite block completely transformed into B-type CO 3 Ap keeping its macroscopic morphology. Diametral tensile strength of the low-crystalline B-type CO 3 Ap block was approximately 5.4 MPa, and this value was much higher when compared with the low-crystalline calcite block, 0.73 MPa. Low crystalline B-type CO 3 Ap block fabricated in this method could be an ideal bone replacement due to its close similarity in its inorganic chemical composition and crystallinity to bone.

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Bone Grafts and Substitutes

Cited by 23 publications

References 0 publications

A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

Human Embryonic Stem Cell-Derived Mesenchymal Stem Cell Seeding on Calcium Phosphate Cement-Chitosan-RGD Scaffold for Bone Repair

Fabrication of low crystalline B-type carbonate apatite block from low crystalline calcite block

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