Bone Graft Biomineral Complex Coderived from Marine Biocalcification and Biosilicification

Abstract

Bone graft materials have been mainly developed based on inorganic materials, including calcium phosphate. However, these graft materials usually act as osteoconductive rather than osteoinductive scaffolds. To improve bone reconstruction, a combination of several materials has been proposed. However, there are still no alternatives that can completely replace the existing animal-derived bone graft materials. In this work, a marine-inspired biomineral complex was suggested as a potential bone graft material. The proposed biosilicified coccolithophorederived coccoliths using bioengineered mussel adhesive proteins show osteopromotive ability through the synergistic effects of osteoconductivity from calcium carbonate and osteoinductivity from silica. Its possibility of use as a bone substitute was determined by evaluating the in vitro osteogenic behaviors of multipotent mesenchymal stem cells and in vivo bone regeneration in a rat calvarial defect model. Therefore, the marine-inspired biomineral complex developed in this study could be successfully used for bone tissue engineering.

A sticky tale : Biomaterial coatings from modular proteins

“…202 Finally, Yun et al prepared a porous biomineral composite material with the help of a bifunctional peptide and marine phytoplankton. 203 The porous calcium carbonate shell of coccolithophores (coccolith) was mixed with the peptide solution, resulting in a silicified material with high in vitro cytocompatibility and osteoinductivity in vivo.…”