Bone formation in calcium-phosphate-coated titanium mesh

Vehof, J.W.M.; Spauwen, P.H.M.; Jansen, John A.

doi:10.1016/s0142-9612(00)00094-6

Cited by 117 publications

(82 citation statements)

References 26 publications

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“…The titanium implants have been proven biocompatible with osteoblasts, chondrocytes, and stromal cells in in vitro studies [7,[35][36][37]. Enhancement of the interface between the implants and cells has been attempted by creating open pores on the implant surface [38], making fiber metal composites [39], and treating the surface of the implants with calcium phosphates [40][41] by varying thermal conditions [42].…”

Section: Discussionmentioning

confidence: 99%

“…Enhancement of the interface between the implants and cells has been attempted by creating open pores on the implant surface [38], making fiber metal composites [39], and treating the surface of the implants with calcium phosphates [40][41] by varying thermal conditions [42]. The interface was evaluated by cell adhesion, proliferation, and differentiation [35,43], as well as by cell morphology and cytoskeleton by scanning or confocal microscopy by synthesis of the proteins of the extracellular matrix and proteoglycans [36]. In vivo, the biocompatibility has been evaluated histologically [7,44].…”

Section: Discussionmentioning

confidence: 99%

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Porous composite prosthetic pylon for integration with skin and bone

Pitkin¹,

Raykhtsaum²,

Pilling³

et al. 2007

JRRD

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Abstract-This article presents results of the further development and testing of the "skin and bone integrated pylon" (SBIP-1) for percutaneous (through skin) connection of the residual bone with an external limb prosthesis. We investigated a composite structure (called the SBIP-2) made of titanium particles and fine wires using mathematical modeling and mechanical testing. Results showed that the strength of the pylon was comparable with that of anatomical bone. In vitro and in vivo animal studies on 30 rats showed that the reinforcement of the composite pylon did not compromise its previously shown capacity for inviting skin and bone cell ingrowth through the device. These findings provide evidence for the safe and reliable long-term percutaneous transfer of vital and therapeutic substances, signals, and necessary forces and moments from a prosthetic device to the body.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Porous composite prosthetic pylon for integration with skin and bone

Pitkin¹,

Raykhtsaum²,

Pilling³

et al. 2007

JRRD

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“…Sintered titanium fiber mesh (TFM) has excellent mechanical properties, can be molded to the required shape and has been shown to be biocompatible (Jansen et al 1992;Vehof et al 2000Vehof et al , 2003. TFM can be used as a carrier for growth factors and bone marrow stromal cells (BMSCs) to induce bone formation (Vehof et al 2000(Vehof et al , 2003.…”

Section: Introductionmentioning

confidence: 99%

“…TFM can be used as a carrier for growth factors and bone marrow stromal cells (BMSCs) to induce bone formation (Vehof et al 2000(Vehof et al , 2003. We previously reported that DFAT cells differentiate into osteoblasts as early as day 7 of culture under three-dimensional (3D) culture conditions (Kishimoto et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Dedifferentiated fat cells differentiate into osteoblasts in titanium fiber mesh

et al. 2012

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Mature adipocyte-derived dedifferentiated fat (DFAT) cells rapidly differentiate into osteoblasts under three-dimensional culture conditions. However, it has not been demonstrated that DFAT cells can differentiate into osteoblasts in a rigid scaffold consisting of titanium fiber mesh (TFM). We examined the proliferation and osteogenic differentiation ability of DFAT cells using TFM as a scaffold. DFAT cells derived from rabbit subcutaneous fat were seeded into TFM and cultured in osteogenic medium containing dexamethasone, L-ascorbic acid 2-phosphate and b-glycerophosphate for 14 days. In scanning electron microscopy (SEM) analysis, well-spread cells covered the titanium fibers on day 3, and appeared to increase in number from day 3 to 7. Numerous globular accretions were found and almost completely covered the fibers on day 14. Cell proliferation, as measured by DNA content in the TFM, was significantly higher on day 7 compared with that of day 1. Osteocalcin and calcium content in the TFM were significantly higher on day 14 compared to those of days 1, 3, and 7, indicating DFAT cells differentiated into osteoblasts. We theorize that globular accretions observed in SEM analysis may be calcified matrix resulting from osteocalcin secreted by osteoblasts binding calcium contained in fetal bovine serum. In this study, we demonstrated that DFAT cells differentiate into osteoblasts and deposit mineralized matrices in TFM. Therefore, the combination of DFAT cells and TFM may be an attractive option for bone tissue engineering.

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“…Certain implant applications of Ti alloy foams have also been recently exploited including spinal cages used in spinal surgery for bone fixation [8]. Since these applications require relatively shorter bone fixation periods onto the porous surface, calcium phosphate (CaP) coating of porous surfaces including Ti mesh [9,10] and the sintered beads surface coatings [11,12] were previously investigated. Various surface treatments were further applied in conjunction with biomimetic CaP coating to fulfill the requirement for in vivo bone growth namely the formation of CaP (bone like apatite).…”

Section: Introductionmentioning

confidence: 99%

The effect of surface treatment on CaP deposition of Ti6Al4V open cell foams in SBF solution

Türkan

Güden

2010

Ceramics International

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The effects of alkali and nitric acid surface treatment and acid etching on the CaP deposition of an open cell Ti6Al4V foam (60% porous and 300-500 mm in pore size) developed for biomedical applications were investigated in a simulated body fluid (SBF) solution for 14-day. The surface roughness of the foam specimens ground flat surfaces was measured in nano-metric scale before and after SBF immersion using an atomic force microscope (AFM). A significant increase in the surface roughness of alkali treated foam specimen after SBF immersion indicated a smaller crystal size CaP deposition, which was also confirmed by the AFM micrographs. The microscopic evaluation clearly showed that alkali treatment and nitric acid treatment induced a continuous, uniform CaP deposition on the cell wall surfaces of the foam (interior of cells). While in untreated foam specimen the cells are filled with CaP precipitates and acid etching did not produce a continuous coating layer on particles interior of the cells. The coating layer thickness was $3 mm in alkali treated foam specimens after 14-day of SBF immersion, while nitric acid treatment induced relatively thinner coating layer, 0.6 mm. #

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Bone formation in calcium-phosphate-coated titanium mesh

Cited by 117 publications

References 26 publications

Porous composite prosthetic pylon for integration with skin and bone

Porous composite prosthetic pylon for integration with skin and bone

Dedifferentiated fat cells differentiate into osteoblasts in titanium fiber mesh

The effect of surface treatment on CaP deposition of Ti6Al4V open cell foams in SBF solution

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