Bone changes caused by experimental Solanum malacoxylon poisoning in rabbits

Abstract

The aim of this study was to describe the bone changes observed after a daily oral administration of the calcinogenic plant Solanum malacoxylon (syn. S. glaucophyllum) (Sm) during 9 days. The Sm-poisoned rabbits had an increase of bone resorption in the endosteal surface of the cortical zone and also in the surface covered by osteoblasts of the primary and secondary spongiosa of the trabecular bone compartment. Moreover, the epiphyseal growth plates in long bones appeared narrower than in the control rabbits, with reduction of the proliferative and hyperthrophic chondrocyte zones. The electron microscopic study revealed a significant decrease of proteoglycans in the hyperthrophic chondrocyte zone evidenced by a significant reduction of rutenium red positive granules in the poisoned rabbit. Altogether, these data suggest that cell differentiation may play a pivotal role in the pathogenesis of Sm-induced bone lesions

Toxic Chemical Compounds of the Solanaceae

“…Mello and Habermehl [170] Cell differentiation damage induced by calcinogenic plants has been analyzed in skin, aorta and lungs [171]. Aguirre et al [172] reported the bone and growth cartilage changes after subacute poisoning of rabbits with S. glaucophyllum. Recently, Gil et al [173] validated a method to estimate the amounts of 1,25-(OH) 2 D 3 in S. glaucophyllum leaves, based on a purification using C 18 minicolumns, and RIA assays with an antibody raised in rabbits by injection of the acid-C 22 , 1α-(OH)vitamin D 3 .…”