Bone augmentation around a dental implant using demineralized bone sheet containing biologically active substances

Shirai, Mai; Yamamoto, Ryûji; Chiba, Toshie; Komatsu, Koichiro; Shimoda, Shinji; Yamakoshi, Yasuo; Oida, Shinichiro; Ohkubo, Chikahiro

doi:10.4012/dmj.2016-026

Cited by 4 publications

(3 citation statements)

References 20 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DBM-based augmentation materials have been used successfully both to improve the bone substrate prior to dental implant insertion [45] and for peri-implant repair surgery [46]; however, they are especially used as a supportive material for a number of biologically active substances [47]. Moreover, the increasing use of DBM in dental applications is related to its use as a transport vehicle for a number of added excipients, such as glycerol, starch, hyaluronic acid or saline, which allows for good maneuverability and improved adaptability [48].…”

Section: Allograftsmentioning

confidence: 99%

Current Options and Future Perspectives on Bone Graft and Biomaterials Substitutes for Bone Repair, from Clinical Needs to Advanced Biomaterials Research

et al. 2023

View full text Add to dashboard Cite

The ideal biomaterials substitute for bone repair should possess the following characteristics: provide osteogenic, osteoinductive and osteoconductive properties; stimulate the neo-angiogenesis process; absence of antigenic, teratogenic or carcinogenic reactions; avoid the systemic toxicity complications; assure satisfactory support and stability from mechanical properties point of view; hydrophilic nature of the surface properties and good interface with human bone; good handling in clinical condition and ability to be easy sterilized; and able to be supplied in sufficient quantities with reduced costs. Despite years of effort, the perfect bone reconstruction material has not yet been developed; further effort is required to make this objective feasible. The aim of this article is to provide a contemporary and comprehensive overview of the grafting materials that can be applied for the treatment of bone defects by the clinicians from orthopedics surgery, neurosurgery and dentistry, discussing their properties, advantages and disadvantages, and illuminating present and future perspectives in the field of bone graft and biomaterials substitutes for bone repair, from clinical needs to advanced biomaterials research.

show abstract

Section: Allograftsmentioning

confidence: 99%

Current Options and Future Perspectives on Bone Graft and Biomaterials Substitutes for Bone Repair, from Clinical Needs to Advanced Biomaterials Research

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Furthermore, the combination of lyophilized bone allografts with platelet-rich fibrin or growth factor-rich plasma has been shown to contribute for the efficacy in alveolar ridge preservation [ 9 , 10 ]. In our previous study, we developed a novel sheet of demineralized rat femur bone (demineralized bone sheet) and demonstrated that bone augmentation was clearly promoted by covering the demineralized bone sheet at the location of the implant immediately after placement into the extraction socket [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of bioactive substances involved in the induction of bone augmentation using demineralized bone sheets

et al. 2022

Self Cite

View full text Add to dashboard Cite

Purpose To investigate the bone augmentation ability of demineralized bone sheets mixed with allogeneic bone with protein fractions containing bioactive substances and the interaction between coexisting bioactive substances and proteins. Methods Four types of demineralized bone sheets mixed with allogeneic bone in the presence or absence of bone proteins were created. Transplantation experiments using each demineralized bone sheet were performed in rats, and their ability to induce bone augmentation was analysed by microcomputed tomography images. Bioactive substances in bone proteins were isolated by heparin affinity chromatography and detected by the measurement of alkaline phosphatase activity in human periodontal ligament cells and dual luciferase assays. Noncollagenous proteins (NCPs) coexisting with the bioactive substances were identified by mass spectrometry, and their interaction with bioactive substances was investigated by in vitro binding experiments. Results Demineralized bone sheets containing bone proteins possessed the ability to induce bone augmentation. Bone proteins were isolated into five fractions by heparin affinity chromatography, and transforming growth factor-beta (TGF-β) was detected in the third fraction (Hep-c). Dentin matrix protein 1 (DMP1), matrix extracellular phosphoglycoprotein (MEPE), and biglycan (BGN) also coexisted in Hep-c, and the binding of these proteins to TGF-β increased TGF-β activity by approximately 14.7% to 32.7%. Conclusions Demineralized bone sheets are capable of inducing bone augmentation, and this ability is mainly due to TGF-β in the bone protein mixed with the sheets. The activity of TGF-β is maintained when binding to bone NCPs such as DMP1, MEPE, and BGN in the sheets.

show abstract

“…The microcracks and fractures on the surface of the bone are believed to be sites of active remodeling 2 . It is well known that bone induction occurred predominantly in demineralized bone matrix (DBM) 3,4,5 . We believe surface area and 3D interconnected porous structure are supreme factors for better cellular performance in bone induction and conduction.…”

Section: Introductionmentioning

confidence: 99%

Microcracks Design and Bone Induction of Skull Bone Modified by Ultrasonic Treatment Using Acidic Electrolyzed Water

Shakya

Murata

Okubo

et al. 2017

Phosphorus Research Bulletin

View full text Add to dashboard Cite

(use 9pt): Healthy bone has many physiological microcracks, which may be involved in the release of bone matrix-derived factors and act to accelerate bone-remodeling process. In this study, the mouse parietal bone fragments (5x5x1mm 3 ) were demineralized by acidic electrolyzed water (AEW: pH2.7) or distilled water (DW: pH5.2) at 120W and 38KHz for 20 min. Each bone was implanted into subcutaneous tissue of 10 week-old male nude mouse, and explanted at 4 and 6 weeks. AEW-bone showed clear enlargement and union of cracks on SEM. AEW-bone revealed active bone induction over wide areas at 6 weeks, while DW-bone induced new bone in limited area. We concluded that the AEW-bone had better performance in bone induction than the DW-bone. Our micro-damage technique combined with AEW and the ultrasonic irradiation will contribute to improve surface area and 3D structure of the dense bone and promote bone formation in the initial stage for bone remodeling.

show abstract

Bone augmentation around a dental implant using demineralized bone sheet containing biologically active substances

Cited by 4 publications

References 20 publications

Current Options and Future Perspectives on Bone Graft and Biomaterials Substitutes for Bone Repair, from Clinical Needs to Advanced Biomaterials Research

Current Options and Future Perspectives on Bone Graft and Biomaterials Substitutes for Bone Repair, from Clinical Needs to Advanced Biomaterials Research

Characterization of bioactive substances involved in the induction of bone augmentation using demineralized bone sheets

Microcracks Design and Bone Induction of Skull Bone Modified by Ultrasonic Treatment Using Acidic Electrolyzed Water

Contact Info

Product

Resources

About