Bonded Fused Silica Capillary Column GLC Determination of BHA and BHT in Chewing Gums

Abstract

A gas chromatographic method was developed for the quantitation of BHA and BHT in toluene plus 2-propanol extracts of chewing gum samples. The method uses a thick film (1.0 micron) bonded fused silica open tubular capillary column which resolves antioxidants from flavor oil volatiles and gum base components as confirmed by mass spectral analysis. The chromatographic method is reproducible at 4.0 ppm and detects as little as 2.0 ppm. Regression analysis of antioxidant-biphenyl internal standard peak area ratios to antioxidant concentrations resulted in a highly linear equation with a correlation coefficient (R) of 0.99 for the ,I0 -150 ppm range.

The Quantitative Determination of Butylated Hydroxytoluene in Chewing Gum Using GC–MS

“…BHT concentrations in commercially-available chewing gums ranged from 42 to 172 µg BHT per g gum, similar to results (i.e., 50-200 µg BHT per g gum) reported in other studies (11)(12)(13)(14). In our experiment, we measured BHT concentrations immediately after sample extraction, as well as several weeks later.…”

Voltammetric Determination of TBHQ Individually and Mixed with BHT in Petroleum Products Using a Gold Disc Electrode

“…Several analytical methods have been reported, including spectrophotometry, − liquid chromatography − (mostly in combination with electrochemical detection , but also with mass spectrometry) , and gas chromatography with various detection methods − for analysis of a wide variety of synthetic antioxidants in different types of samples. Generally, chromatographic methods are used for the analysis of these compounds in food, − but even then, prior separation or sample clean up is sometimes required, resulting in fairly complicated procedures.…”

Utility of 4‐Chloro‐7‐Nitrobenzofurazan for the Spectrofluorimetric Determination of Butylated Hydroxyanisole and Propyl Gallate in Foodstuffs

“…High‐performance liquid chromatography (HPLC) has been the most widely used method for the determination of synthetic antioxidants. Reversed‐phase HPLC provides high selectivity for the separation of synthetic antioxidants under mild chromatographic conditions (Ruiz and others 1999; Aparicio and others 2000; Chen and others 2011), thin‐layer chromatography (Sin and others 2006), gas chromatography (Min and Schweizer 2006), gas liquid chromatography (Greenberg and others 2006), and micellar electrokinetic capillary chromatography analytical methods have also been used for SPA analysis (Wang and others 2010).…”