Boletaceae in China: Taxonomy and phylogeny reveal a new genus, two new species, and a new record

Abstract

Boletaceae, the largest family in Boletales, has been attracted by mycologists in the world due to its diverse morphology and complex history of evolution. Although considerable work has been done in the past decades, novel taxa are continually described. The current study aimed to introduce three new taxa and one new record of Boletaceae from China. The morphological descriptions, color photographs, phylogenetic trees to show the positions of the taxa, and comparisons with allied taxa are provided. The new genus Hemilanmaoa is unique in the Pulveroboletus group, and Hemilanmaoa retistipitatus was introduced as the type species. It can be distinguished by its bluing basidioma when injured, a decurrent hymenophore, a stipe covered with distinct reticulations, and a fertile stipitipellis. Porphyrellus pseudocyaneotinctus is characterized by its pileipellis consisting of broadly concatenated cells and thin-walled caulocystidia in Porphyrellus. In Phylloporus, Phylloporus biyangensis can be distinguished by its hymenophores that change to blue when injured and yellow basal mycelium. Lanmaoa angustispora, as a new record, is first reported in Northern China. Internal transcribed spacer (ITS), 28S rDNA (28S), translation elongation factor 1-alpha (tef1-α), RNA polymerase II subunit 1 (rpb1), and RNA polymerase II subunit 2 (rpb2) were employed to execute phylogenetic analyses.

Concordance of multigene genealogy along with morphological evidence unveils five novel species and two new records of boletoid mushrooms (fungi) from India

“…orientifumosipes differs mostly from the present species by longer tubes (up to 20 mm), presence of ring-like bluish zone at stipe apex, smaller basidiospores (9.5–10.5 × 4.5–5.5 µm) and much longer hymenial cystidia (58–74 × 15–19 µm) whereas, Por. pseudocyaneotinctus shows distinctively more robust basidiomata (pileus 46–99 mm in diameter, stipe 48–123 × 9–19 mm), larger hymenial cystidia (pleurocystidia 36.8–85 × 8.5–13 µm, cheilocystidia 38.2–60.5 × 10.8–17.9 µm), differently-shaped cheilocystidia (lageniform), differently-shaped terminal elements of pileipellis hyphae and a sterile stipitipellis 18 , 38 .…”

“…Smith, Costanzo, & Vizzini, Rostrupomyces Vadthanarat & Raspé, Rubinosporus Vadthanarat, Raspé & Lumyong, Spongispora G. Wu, S.M.L. Lee, E. Horak & Zhu L. Yang, Tropicoboletus Angelini, Gelardi & Vizzini and Villoboletus L. Fan & N. Mao 37 , 38 , 47 – 63 . It is noteworthy that 11 out of 22 genera are established from Asian countries like, China, Thailand and India.…”

“…The ITS, LSU, rpb2 and tef 1-α sequences of the newly generated Leccinellum bothii , L. sinoaurantiacum , Phylloporus himalayanus , P. smithii , Xerocomus rugosellus , Porphyrellus uttarakhandae and Retiboletus pseudoater and their close relatives were retrieved from nBLAST search against GenBank ( https://www.ncbi.nlm.nih.gov/genbank ) and relevant published phylogenies 5 , 16 , 18 , 22 – 24 , 36 – 38 . Four raw datasets (ITS, LSU, rpb2 and tef 1-α) were created separately.…”

“…nih. gov/ genba nk) and relevant published phylogenies5,16,18,[22][23][24][36][37][38] . Four raw datasets (ITS, LSU, rpb2 and tef 1-α) were created separately.…”