Boesenbergia rotunda displayed anti-inflammatory, antioxidant and anti-apoptotic efficacy in doxorubicin‐induced cardiotoxicity in rats

Abstract

This study evaluated the cardioprotective properties of Boesenbergia rotunda extract (BrE) against doxorubicin (DOX) induced cardiotoxicity. Rats received oral gavage of BrE for 28 days and DOX (5 mg/kg/week for 3 weeks). Thereafter the animals were sacrificed, blood and cardiac samples were collected for biochemical, histological and immunohistochemical analyses. The results indicated that BrE attenuated DOX triggered body and cardiac weight loss and prevented against cardiac injury by mitigating histopathological alterations in cardiac tissues as well as serum cardiac function enzymes. BrE significantly reduced serum levels of aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), troponin T (TnT) and creatine kinase-MB (CK-MB) in DOX-treated rats. Furthermore, BrE alleviated cardiotoxicity by reducing DOX instigated oxidative stress and potentiating the level of glutathione, as well as the activities superoxide dismutase and catalase in cardiac tissues. In addition, BrE significantly decreased the characteristic indices of DOX-induced cardiac inflammation and apoptosis. Immuno-histochemical analysis revealed that BrE decreased the stain intensity of p53 and myeloperoxidase (MPO) proteins compared to the DXB alone group. In conclusion, our results indicated that BrE modulated oxidative stress, inflammation and apoptosis to attenuate DOX-induced cardiac damage.

Oral sub-chronic toxicity of fingerroot (Boesenbergia rotunda) rhizome extract formulation in Wistar rats

“…( Fig. 1 A), a member of the Zingiberaceae family, commonly known as fingerroot or referred to as Boesenbergia pandurata , Kaempferia pandurata , is widely used in Southeast Asia for culinary and traditional medicinal purposes [1] . Research on the rhizome extracts of this plant ( Fig.…”

“…Chronic inflammation caused by exaggerated and prolonged inflammatory responses might lead to cardiovascular disease, type 2 diabetes, rheumatoid arthritis, and cancers [14] , [15] . Fingerroot extract possesses cardioprotective effects by mitigating this inflammation [1] . An ethanolic extract of B. rotunda could inhibit the expression of Akt and nuclear factor kappa (NF)-κB p65 in the stomach and intestine of acetic acid–induced Wistar rats [16] .…”