Body fluid cellular analysis using the Sysmex XN‐2000 automatic hematology analyzer: focusing on malignant samples

Abstract

The BF mode on the Sysmex XN could be an alternative method for the manual counts in the BF analysis with a few drawbacks. However, if a concentration of HF-BF cells is greater than the given threshold, microscopic examination should be subsequently performed.

Evaluation of Sysmex XN-1000 hematology analyzer for cell count and screening of malignant cells of serous cavity effusion

“…[15] The cell classification by instrumental method and manual staining microscopy method had a high correlation, PMN% was similar to MN%, both were 0.90, respectively. This finding was consistent with the report of Cho et al, [7] but slightly weaker than what was reported by Fleming et al The main reason for this was that the testing specimens had a high proportion of peritoneal dialysis fluid, and the test was not focused on malignant effusions, which resulted in a great improvement in the correctness of the instrument. The cut-off values of HFC% and HFC# were 4.4% and 24.5/μL, respectively, whereas the values for AUC and sensitivity and specificity were 0.707, 0.792, 0.558 and 0.708, 0.753, 0.550, respectively, which were slightly lower than what was reported in the literature.…”

“…The correlation coefficients of RBC and NUC were 0.99 and 0.98, respectively, which were consistent with the reported literature. [5,7] XN-1000 hematology analyzer body fluid mode is capable of classifying and counting nucleated cells, including neutrophils, lymphocytes, monocytes, eosinophils, and HFC. The neutrophils, eosinophils, and basophils were classified as polymorphocytes (PMN), and lymphocytes, macrophages, mesothelial cells, and malignant cells were classified as monocytes (MN).…”

“…The cut-off values of HFC% and HFC# were 4.4% and 24.5/μL, respectively, whereas the values for AUC and sensitivity and specificity were 0.707, 0.792, 0.558 and 0.708, 0.753, 0.550, respectively, which were slightly lower than what was reported in the literature. [6,7] During chronic inflammation, the number of mesothelial cells and macrophages were significantly increased in the effusion, and their nuclei contained more nucleic acids, which can be combined with more nucleic acid fluorescent dyes, and thus can be classified as high-fluorescent nucleated cells. In this experiment, we observed that liver cirrhosis ascites specimens contained many high-fluorescence nucleated cells, which were mainly classified as mesothelial cells and/or macrophages by microscopic examination Fig.…”

“…Cell counting and classification in independent body fluid analysis method in Sysmex XE-5000 and XN-1000 hematology analyzer (Sysmex Corporation, Kobe, Japan) are carried using semiconductor laser flow cytometry and nucleic acid fluorescence staining techniques. There have been a few reports on the performance evaluation of body fluid mode and malignant cell screening, [4–7] the evidence to validate the efficacy of automatic nucleated cell counting, nucleated classification, and malignant cell screening of serous cavity effusion is still very rare.…”

Evaluation of Mindray BC-6800 body fluid mode for automated cerebrospinal fluid cell counting

“…Therefore, a simple, fast and inexpensive test like the total nucleated cell count and differentiation can be of great help in providing clinicians with useful diagnostic information on CNS conditions [1][2][3][4][5][6][7][8][9].…”