BODIPY‐Based Fluorescent Thermometer as a Lysosome‐Targetable Probe: How the Oligo(ethylene glycols) Compete Photoinduced Electron Transfer

Principle has it that even the most advanced super-resolution microscope would be futile in providing biological insight into subcellular matrices without well-designed fluorescent tags/probes. Developments in biology have increasingly been boosted by advances of chemistry, with one prominent example being small-molecule fluorescent probes that not only allow cellular-level imaging, but also subcellular imaging. A majority, if not all, of the chemical/biological events take place inside cellular organelles, and researchers have been shifting their attention towards these substructures with the help of fluorescence techniques. This Review summarizes the existing fluorescent probes that target chemical/biological events within a single organelle. More importantly, organelle-anchoring strategies are described and emphasized to inspire the design of new generations of fluorescent probes, before concluding with future prospects on the possible further development of chemical biology.

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“…In addition, a lysosome‐targeting fluorescent thermometer was synthesized by Wang et al. by using a similar strategy …”

Section: Targeting and Activatable Fluorescence Probes For The Cellumentioning

confidence: 99%

Discerning the Chemistry in Individual Organelles with Small‐Molecule Fluorescent Probes

et al. 2016

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“…Other organic probes displaying changes in fluorescence intensity with temperature are based on molecular rotors (or more recently mechanically induced change in emission). These include 8‐phenyl boron‐dipyrromethenes (BODIPYs), boryated arylisoquinolines, and bisnaphthalimides . Similar to 1 , these dyes have a portion that can rotate around a single bond accessing a dark state, resulting in a reduction in the fluorescence intensity and often the excited state lifetime.…”

Section: Emission Intensitymentioning

confidence: 99%

“…Wang et al . synthesized two PEGylated BODIPY dyes having a free rotating phenyl ring (Figure , structure 5 and 6 ) . 5 has no steric hindrance to slow the rotation of the phenyl ring, while 6 has two methyl groups, which hinder the rotation considerably.…”

Section: Emission Intensitymentioning

confidence: 99%

Latest Trends in Temperature Sensing by Molecular Probes

et al. 2020

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In this Review, we summarize the work in the last ten years on the development of fluorescent molecular probes with possible applications in microscopic thermometry. We discuss four principal types of temperature‐dependent photoluminescence features: 1) Emission intensity; 2) Ratio of emission intensities; 3) Emission peak shift; and 4) Emission lifetime. We compare the advantages, limitations, and challenges of the different probes using these types of sensing mechanisms. By focusing on molecular probes, rather than nanoparticles or polymers, the mechanisms of temperature dependence are discussed thoroughly, with the most common including twisted intramolecular charge transfer (TICT), aggregation‐induced emission (AIE), and mechanically induced change in emission (MICE). With many different confounding variables associated with each experimental method and mechanism of temperature‐dependent photoluminescence, lanthanide and transition metal complexes seem to be promising candidates for future microscopic thermometry applications.

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“…Zusätzlich wurde basierend auf einer ähnlichen Strategie von Wang et al. über ein auf Lysosomen zielendes Fluoreszenzthermometer berichtet …”

Section: Auf Das Zelluläre Verdauungssystem Zielende Aktivierbare Flunclassified

Wahrnehmung der chemischen Prozesse in einzelnen Organellen mit niedermolekularen Fluoreszenzsonden

et al. 2016

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Ohne genau durchdachte Fluoreszenzmarker/‐sonden kann auch das fortschrittlichste hochauflösende Mikroskopieverfahren keinen Einblick in subzelluläre Matrizes geben. Die Entwicklung der Biologie wurde in zunehmendem Maße durch Fortschritte im Bereich der Chemie vorangetrieben. Ein prominentes Beispiel hierfür sind niedermolekulare Fluoreszenzsonden, die nicht nur die Bildgebung auf zellulärer Ebene ermöglichen, sondern auch subzelluläre Abbildungen. Die meisten chemischen/biologischen Ereignisse finden innerhalb von Zellorganellen statt, sodass diese Substrukturen zunehmend mithilfe von Fluoreszenzverfahren untersucht wurden. Dieser Aufsatz fasst die vorhandenen Fluoreszenzsonden zusammen, die auf chemische/biologische Geschehnisse innerhalb einzelner Organellen zielen. Darüber hinaus werden Strategien zur Verankerung an den Organellen vorgestellt, um das Design neuer Fluoreszenzsonden anzuregen. Abschließend werden die Zukunftsaussichten für weitere Entwicklungen in der chemischen Biologie diskutiert.

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BODIPY‐Based Fluorescent Thermometer as a Lysosome‐Targetable Probe: How the Oligo(ethylene glycols) Compete Photoinduced Electron Transfer

Cited by 33 publications

References 32 publications

Discerning the Chemistry in Individual Organelles with Small‐Molecule Fluorescent Probes

Discerning the Chemistry in Individual Organelles with Small‐Molecule Fluorescent Probes

Latest Trends in Temperature Sensing by Molecular Probes

Wahrnehmung der chemischen Prozesse in einzelnen Organellen mit niedermolekularen Fluoreszenzsonden

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