Boar spermatozoa in the oviduct

Rodríguez‐Martínez, H.; Saravia, F.; Wallgren, Margareta; Tienthai, Paisan; Johannisson, Anders; Vázquez, Juan M.; Martı́nez, Emilio A.; Roca, Jordi; Sanz, Libia; Calvete, Juan J.

doi:10.1016/j.theriogenology.2004.09.028

Cited by 195 publications

(202 citation statements)

References 78 publications

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“…However, some 1-2 hr before ovulation, as distinct from after collapse of the ovulating follicle(s), strictly limited numbers of spermatozoa in the reservoir commence to be reactivated and released from binding, progressing towards the site of fertilisation in discrete numbers. Rodriguez-Martinez et al (2005) suggest that there may be a continuous redistribution towards the upper isthmus in pigs, but it is not easy to reconcile this view with the low initial sperm/egg ratios observed close to the time of ovulation nor with the small number of accessory spermatozoa found on or in the zona pellucida of newly penetrated eggs. Increasing numbers of spermatozoa are activated and released from the reservoir during the actual process of ovulation, enhancing the numbers progressing towards the ampullary-isthmic junction.…”

Section: Contrasts In Sperm Motilitymentioning

confidence: 99%

“…For example, bovine spermatozoa bind to fucose-containing molecules on the surface of the caudal isthmus Suarez, 1998Suarez, , 2001Revah et al, 2000) whereas complex mannose structures appear to be involved in the initial sperm binding mechanism in pig oviducts . Recent reviews on the functional sperm reservoir have been given by Suarez (2001), Töpfer-Petersen et al (2002) and Rodriguez-Martinez et al (2005).…”

Section: Functional Sperm Reservoirmentioning

confidence: 99%

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Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Hunter

2007

Molecular Reproduction Devel

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To avoid inappropriate conclusions being drawn from the extensive use of in vitro preparations of sperm-oviduct epithelial binding, it is recalled that events in the genital tract of mammals are regulated by the gonads, primarily by their changing secretion of steroid hormones. Key observations from in vivo models are used to emphasise the dynamic interactions between viable sperm cells and the caudal (distal) portion of the oviduct isthmus, the site of the functional sperm reservoir. These include (1) preovulatory arrest and epithelial binding of intact sperm cells and thereby suppression of completion of capacitation, (2) peri-ovulatory activation and release from binding of discrete sub-populations of competent spermatozoa, and (3) post-ovulatory liberation of large numbers of spermatozoa. These observations underline the influence of endocrine regulation of sperm binding and release by peri-ovulatory Graafian follicles, a point brought out by the enhanced sperm release prompted by diverse treatments with solutions of progesterone. In the light of this evidence, the suitability of in vitro preparations for clarifying physiological events should be questioned, especially if myosalpingeal catecholamines diffusing out of the autonomic nervous system contribute to sperm activation and/or release. None of this is to infer that sperm cells themselves are without influence on their epithelial binding reaction(s). Nor is it to suggest that in vitro models of sperm-oviduct binding are without relevance to the development of sperm evaluation technologies. However, pre-ovulatory sperm-epithelial binding and a regulated peri-ovulatory release should be seen as vital tactics in the overall strategy of achieving successful monospermic fertilisation. Mol. Reprod. Dev. 75: 167-174, 2008.

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Section: Contrasts In Sperm Motilitymentioning

confidence: 99%

Section: Functional Sperm Reservoirmentioning

confidence: 99%

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Hunter

2007

Molecular Reproduction Devel

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“…without inducing acrosome exocytosis or cell death [38,39]. The concerted evidence emanated from experimental findings, albeit without explanation of the mechanisms behind, all converse to conclude that immersion in hyaluronan-containing (tubal) fluid is beneficial for sperm survival, maybe simply delaying the permissive capacitation process [23,40]. Further mechanistic studies are thus needed to discern how we can manipulate sperm stability using hyaluronan.…”

Section: The Stored Tubal Spermatozoa and Their Relation To The Tubalmentioning

confidence: 99%

The ubiquitous hyaluronan: Functionally implicated in the oviduct?

et al. 2016

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Hyaluronan (hyaluronic acid) is a simple, non-antigenic, non-sulphated glycosaminoglycan (GAG) present everywhere in the extracellular compartments of the body. Noteworthy, it is highly conserved phylogenetically, from sauropsida to mammals; and plays a plethora of roles from embryonic/fetal development to adult physiological and pathological events, including tumor development. In reproduction, hyaluronan has proven related to initial events as sperm survival, build-up of the sperm reservoir in the oviduct, regulation of sperm capacitation and pre-fertilization to later participate in embryo, fetal and placental development. Synthesis, binding (via the CD44 membrane receptor) and degradation of hyaluronan occur in male and female genital organs, the oviduct being no exception. This review discusses our current knowledge on roles of this ubiquitous GAG on the survival of immunologically foreign spermatozoa in the pig oviduct, a relevant event for fertility. During pre-ovulatory storage in the functional tubal sperm reservoir, spermatozoa are entrapped in a mucus-like tubal fluid.This fluid contains fluctuating levels of hyaluronan, which is synthesized by the lining 18th ICAR-2016-S2. Pig reproduction Symp 2 epithelium by HA-synthase 3 (has3). Both hyaluronan and its CD44-receptor are particularly evident in the deep mucosal furrows of the sperm reservoir, where most spermatozoa are embedded in; kept alive, un-capacitated but also undetected by the immune system of the female. Hyaluronan is also present in the seminal plasma, and evidence points out an involvement of hyaluronan and its receptor in the local (tubal and possibly uterine) production of anti-inflammatory cytokines, such as IL-10, pertaining maternal immune tolerance of these foreign cells.

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“…In porcine sperm, the concentration of extracellular bicarbonate increases from about 2 mM in the cauda epididymal fluid to over 20 mM in semen, which activates bicarbonate-sensitive, soluble adenylyl cyclase, resulting in the stimulation of O 2 consumption and in the initiation of coordinated forward movement of sperm (Tajima et al 1987). The concentration of bicarbonate further increases to above 30 mM in the oviduct fluids, where sperm acquire fertilizing capacity through the second activation process, namely 'capacitation' (Maas et al 1977, Rodríguez-Martínez et al 2005. In addition, although fructose is the predominant respiratory substrate for sperm in semen, it is then replaced by glucose in the oviduct fluids (Mann 1946, Hugentobler et al 2008, Larose et al 2012.…”

Section: Role Of Aldose Reductase In Sperm Capacitationmentioning

confidence: 99%

Porcine sperm capacitation involves tyrosine phosphorylation and activation of aldose reductase

Katoh¹,

Takebayashi²,

Kikuchi³

et al. 2014

Reproduction

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Mammalian sperm must be activated in the tubal isthmus through capacitation to induce the acrosome reaction and subsequent fertilization. Although the molecular mechanisms involved in capacitation have yet to be fully elucidated, increased concentrations of reactive oxygen species (ROS) and the extent of tyrosine phosphorylation of proteins have been suggested to play central roles in the completion of capacitation. In this study, aldose reductase was for the first time identified as one of the tyrosine-phosphorylated proteins involved in the capacitation of porcine cauda epididymal sperm. Both tyrosine phosphorylation and activity of aldose reductase associated with the particulate fraction of sperm cells were significantly enhanced during capacitation. Alrestatin, a membranepermeable and specific inhibitor of aldose reductase, plays a role in the inhibition of aldose reductase activity, elevation of intracellular levels of ROS, and induction of hyperactivated motility, all at similar dose dependencies. Alrestatin canceled both the increase in the tyrosine phosphorylation of aldose reductase and the decrease in the glutathione levels in sperm-induced during capacitation. The hyperactivated motility was induced to a higher extent in the presence of glucose than in the presence of fructose. These results indicate that aldose reductase plays an important role in induction of hyperactivation and capacitation of sperm through the elevation of ROS in sperm cells. Furthermore, aldose reductase was shown to be added to sperm during transit through the epididymis, suggesting that aldose reductase is one of the key proteins that support the functional maturation of sperm.

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Boar spermatozoa in the oviduct

Cited by 195 publications

References 78 publications

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

The ubiquitous hyaluronan: Functionally implicated in the oviduct?

Porcine sperm capacitation involves tyrosine phosphorylation and activation of aldose reductase

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