BMP2 initiates chondrogenic lineage development of adult human mesenchymal stem cells in high-density culture

Schmitt, Bernhard M.; Ringe, Jochen; Häupl, Thomas; Notter, Michael; Manz, Rudi; Burmester, Gerd‐Rüdiger; Sittinger, Michael; Kaps, Christian

doi:10.1111/j.1432-0436.2003.07109003.x

Cited by 208 publications

(178 citation statements)

References 47 publications

(49 reference statements)

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“…Hence, in the present study, instead of ITS in chondrogenic medium supplemented with IGF, only transferrin and selenium were used. Combined with the knowledge that BMP signalling is required for the formation of precartilagenous condensation and differentiation of chondroprecursors and, in adult articular cartilage homeostasis, by enhancing proteoglycan and type II collagen synthesis (Zhang et al, 2002;Schmitt et al, 2003;Noel et al, 2004), the extent of hUCMSC chondrogenesis by these growth factors, when administered singly or in combination with BMP2, is evaluated after 28 d culture with the respective growth factors.…”

Section: Discussionmentioning

confidence: 99%

Significance of soluble growth factors in the chondrogenic response of human umbilical cord matrix stem cells in a porous three dimensional scaffold

Nirmal

Nair²

2013

eCM

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Stem cell based tissue engineering has emerged as a promising strategy for articular cartilage regeneration. Foetal derived mesenchymal stem cells (MSCs) with their ease of availability, pluripotency and high expansion potential have been demonstrated to be an attractive cell source over adult MSCs. However, there is a need for optimisation of chondrogenic signals to direct the differentiation of these multipotent MSCs to chondrogenic lineage. In this study we have demonstrated the in vitro chondrogenesis of human umbilical cord matrix MSCs in three dimensional PVA-PCL (polyvinyl alcoholpolycaprolactone) scaffolds in the presence of the individual growth factors TGFβ1, TGFβ3, IGF, BMP2 and their combination with BMP2. Gene expression, histology and immunohistology were evaluated after 28 d culture. The induced cells showed the feature of chondrocytes in their morphology and expression of typical chondrogenic extracellular matrix molecules. Moreover, the real-time PCR assay has shown the expression of gene markers of chondrogenesis, SOX9, collagen type II and aggrecan. The expression of collagen type I and collagen type X was also evaluated. This study has demonstrated the successful chondrogenic induction of human umbilical cord MSCs in 3D scaffolds. Interestingly, the growth factor combination of TGF-β3 and BMP-2 was found to be more effective for chondrogenesis as shown by the real-time PCR studies. The findings of this study suggest the importance of using growth factor combinations for successful chondrogenic differentiation of umbilical cord MSCs.

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Section: Discussionmentioning

confidence: 99%

Significance of soluble growth factors in the chondrogenic response of human umbilical cord matrix stem cells in a porous three dimensional scaffold

Nirmal

Nair²

2013

eCM

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“…Specifically, BMP-2 is expressed in the condensing mesenchyme of the developing limb [138], and regulates chondrogenic development of mesenchymal progenitors [139][140][141]. BMPs were demonstrated to induce chondrogenesis of MSCs in vitro [142,143]. An important observation in some of these studies is further development towards hyperproliferative state indicating potential final differentiation to bone [114,144].…”

Section: Vivomentioning

confidence: 99%

“…An important observation in some of these studies is further development towards hyperproliferative state indicating potential final differentiation to bone [114,144]. The exclusive development towards chondrogenesis was observed only upon administration of recombinant BMP2 in pellet cultures of human MSC [142]. The exposure of equine MSCs to TGFβ-1 resulted in higher collagen II expression in monolayer cultures [145], and was superior to the treatment with hyaluronic acid and synovial fluid for chondrogenesis in pellet cultures [146].…”

Section: Vivomentioning

confidence: 99%

Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

210

156

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Pain in the joint is often due to cartilage degeneration and represents a serious medical problem affecting people of all ages. Although many, mostly surgical techniques, are currently employed to treat cartilage lesions, none has given satisfactory results in the long term. Recent advances in biology and material science have brought tissue engineering to the forefront of new cartilage repair techniques. The combination of autologous cells, specifically designed scaffolds, bioreactors, mechanical stimulations and growth factors together with the knowledge that underlies the principles of cell biology offers promising avenues for cartilage tissue regeneration. The present review explores basic biology mechanisms for cartilage reconstruction and summarizes the advances in the tissue engineering approaches. Furthermore, the limits of the new methods and their potential application in the osteoarthritic conditions are discussed.

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“…Many experiments have proved that MSCs can differentiate into many kinds of tissues (of bone, cartilage, lipocyte, neurocyte, hematopoietic cell, muscle cell, etc.) (Schmitt et al, 2003;Wang et al, 2004;. Can MSCs differentiate into cardiomyocyte?…”

Section: Discussionmentioning

confidence: 99%

Allograftic bone marrow-derived mesenchymal stem cells transplanted into heart infarcted model of rabbit to renovate infarcted heart

Wang

Fan

et al. 2004

J. Zheijang Univ.-Sci.

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Abstract:Objective: To investigate the directed transplantation of allograftic bone marrow-derived mesenchymal stem cells (MSCs) in myocardial infarcted (MI) model rabbits. Materials and Methods: Rabbits were divided into 3 groups, heart infarcted model with MSCs transplanted treatment (MSCs group, n=12), heart infarcted model with PBS injection (control group, n=20), sham operation with PBS injection (sham group, n=17). MSCs labelled by BrdUrd were injected into the MI area of the MSCs group. The same volume of PBS was injected into the MI area of the control group and sham group. The mortality, LVIDd, LVIDs and LVEF of the two groups were compared 4 weeks later. Tropomyosin inhibitory component (Tn I) and BrdUrd immunohistochemistry identified the engrafted cells 4 weeks after transplantation. Result: The mortality of the MSCs group was 16.7% (2/12), and remarkably lower than the control group's mortality [35% (7/20) (P<0.05)]. Among the animals that survived for 4 weeks, the LVIDd and LVIDs of the MSCs group after operation were 1.17±0.21 cm and 0.74±0.13 cm, and remarkably lower than those of the model group, which were 1.64±0.14 cm and 1.19±0.12 cm (P<0.05); the LVEF of the MSCs group after operation was (63±6)%, and remarkably higher than that of the model group, which was (53±6)% (P<0.05). Among the 10 cases of animals that survived for 4 weeks in the MSCs group, in 8 cases (80%), the transplanted cells survived in the non MI, MI region and its periphery, and even farther away; part of them differentiated into cardiomyocytes; in 7 cases (70%), the transplanted cells participated in the formation of blood vessel tissue in the MI region. Conclusion: Transplanted allograftic MSCs can survive and differentiate into cardiomyocytes, form the blood vessels in the MI region. MSCs transplantation could improve the heart function after MI.

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BMP2 initiates chondrogenic lineage development of adult human mesenchymal stem cells in high-density culture

Cited by 208 publications

References 47 publications

Significance of soluble growth factors in the chondrogenic response of human umbilical cord matrix stem cells in a porous three dimensional scaffold

Significance of soluble growth factors in the chondrogenic response of human umbilical cord matrix stem cells in a porous three dimensional scaffold

Cartilage tissue engineering for degenerative joint disease☆

Allograftic bone marrow-derived mesenchymal stem cells transplanted into heart infarcted model of rabbit to renovate infarcted heart

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