BMP-like signals are required after the midblastula transition for blood cell development

Zhang, Chaohui; Evans, Todd

doi:10.1002/(sici)1520-6408(1996)18:3<267::aid-dvg7>3.0.co;2-7

Cited by 73 publications

(53 citation statements)

References 53 publications

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“…Conversely, analysis of mutants with increased Bmp signaling, combined with overexpression experiments in Xenopus, have shown that Bmp signaling is also sufficient for the formation of blood, vascular and kidney tissues, which are derived from the ventral mesoderm in zebrafish (Kimmel et al, 1990). For example, overexpression of Bmp RNAs in Xenopus animal caps is sufficient to induce the expression of red blood cell markers (Zhang and Evans, 1996), and chordino, a zebrafish mutant with excess Bmp signaling, forms extra blood, kidney and vascular cells Leung et al, 2005). Thus, Bmp signaling is important for the induction of ventral mesodermal derivatives during development.…”

Section: Introductionsupporting

confidence: 85%

Sustained Bmp signaling is essential for cloaca development in zebrafish

et al. 2006

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Bone morphogenetic protein (Bmp) signaling has long been known to be important for the early development of the ventral mesoderm, including blood, vasculature and kidney cells. Although Bmp genes are continually expressed in the ventral cells throughout gastrulation and somitogenesis, previous studies in zebrafish have not addressed how the role of Bmp signaling changes over time to regulate ventral mesoderm development. Here, we describe the use of a transgenic inducible dominantnegative Bmp receptor line to examine the temporal roles of Bmp signaling in ventral mesoderm patterning. Surprisingly, we find that Bmp signaling from the mid-gastrula stage through early somitogenesis is important for excluding blood and vascular precursors from the extreme ventral mesoderm, and we show that this domain is normally required for development of the cloaca (the common gut and urogenital opening). Using a novel assay for cloacal function, we find that larvae with reduced mid-gastrula Bmp signaling cannot properly excrete waste. We show that the cloacal defects result from alterations in the morphogenesis of the cloaca and from changes in the expression of genes marking the excretory system. Finally, we show that HrT, a T-box transcription factor, is a Bmp-regulated gene that has an essential function in cloacal development. We conclude that sustained Bmp signaling plays an important role in specification of the zebrafish cloaca by maintaining the fate of extreme ventral cells during the course of gastrulation and early somitogenesis. Furthermore, our data suggest that alterations in Bmp signaling are one possible cause of anorectal malformations during human embryogenesis.

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Section: Introductionsupporting

confidence: 85%

Sustained Bmp signaling is essential for cloaca development in zebrafish

et al. 2006

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“…Each of the subsequent expression plasmids were then generated by subcloning into the parental I-SceI plasmid, using inserts derived from plasmids for lmo2:gfp (Zhu et al, 2005), gata1:gfp (Long et al, 1997), CA-XBMPR (Candia et al, 1997) and pTurbo-Cre (Hug et al, 1996) inserts. The lmo2:⌬BR and gata1:⌬BR vectors were generated by PCR amplifying the ⌬BR insert described previously (Zhang and Evans, 1996) in place of GFP. Likewise, the lmo2:caBR and lmo2:cre vectors were generated by subcloning the respective cDNAs in place of GFP.…”

Section: Plasmidsmentioning

confidence: 79%

“…Digoxigenin-labeled RNA antisense probes were described previously for gata1 (Detrich et al, 1995); scl (Liao et al, 1998); pax2.1 (Krauss et al, 1991); lmo2 (Zhu et al, 2005); flk1 (Thompson et al, 1998); L-plastin (lcp1 -Zebrafish Information Network) (Herbomel et al, 1999); mpo (Bennett et al, 2001); and no tail (Schulte-Merker et al, 1992). The ⌬br digoxigenin-labeled antisense RNA probe was generated from the Xenopus ⌬BR cDNA clone (Zhang and Evans, 1996). Two-color wholemount in situ hybridization was preformed essentially as described previously (Westerfield, 1995), although instead of Fast Red, INT Red (Sigma) was used to develop the red color.…”

Section: Whole Mount In Situ Hybridizationmentioning

confidence: 99%

BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis

et al. 2006

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The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.

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“…Even if one considers only members of the BMP family, the importance of these molecules is evident. For example, BMP signaling is required for the viability of the preimplantation embryo (Zhao 2003), patterning the embryonic mesoderm (Zhao 2003), establishment of the dorsalventral axis in the invertebrate and vertebrate embryo (Shimmi and O'Connor 2003), early and later stages of hematopoiesis (Zhang and Evans 1996), neural crest cell formation (Tribulo et al, 2003), kidney development (Godin et al, 1999), heart development (Delot et al, 2003), limb patterning (Soshnikova et al, 2003), chondrogenesis…”

Section: Discussionmentioning

confidence: 99%