Sustained Bmp signaling is essential for cloaca development in zebrafish

Pyati, Ujwal J.; Cooper, Mark S.; Davidson, Alan J.; Nechiporuk, Alexei; Kimelman, David

doi:10.1242/dev.02388

Cited by 82 publications

(96 citation statements)

References 39 publications

(37 reference statements)

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“…In zebrafish the presumptive cloaca or proctodeum forms from a single vacuolated cell, to which the pronephros fuses first, followed by the later developing posterior gut (Pyati et al, 2006). The gut and pronephros have discrete openings into the cloaca, which starts out as an upturned U-shape and then After treatment from 24-48 hpf, the posterior gut is thinner than in WT embryos, and despite the appearance of a fistula (C), the majority of embryos have a perforate gut, while 33% (4/12) were imperforate and the dye stayed in the blind ended posterior gut (D).…”

Section: Discussionmentioning

confidence: 99%

“…4L). Cloacal expression of evx1 and prdm1 is lost embryos with impaired BMP signalling (Pyati et al, 2006) whereas in syu and smu mutants it persists, highlighting the altered shape of the cloaca in both mutants (Fig. 5).…”

Section: Shha and Smo Mutant Fish Have Cloacal Abnormalitiesmentioning

confidence: 95%

“…Recently Pyati et al (2006) presented the first detailed description of zebrafish cloacal development and demonstrated an early requirement for Bmp signalling in specification of the presumptive cloaca. Fish deficient in Bmp activity have defects in the opening of the pronephric ducts (kidney terminus) and the posterior gut, which was attributed to an overall failure in the external opening of the cloaca.…”

Section: Introductionmentioning

confidence: 99%

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Hedgehog signalling is required for cloacal development in the zebrafish embryo

Parkin¹,

Allen²,

Ingham³

2009

Int. J. Dev. Biol.

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The Hedgehog (Hh) family of signalling molecules is essential for a wide range of developmental processes. Mammalian studies have implicated the Hedgehog pathway in the aetiology of anorectal malformations (ARMs), relatively common congenital anomalies caused by failures in the development of the cloaca. In this study we demonstrate that Hh signalling is absolutely required for the formation of the zebrafish cloaca and that the severity of the posterior gut abnormalities induced by a reduction in Hh activity is dependent on the levels of Hh signal transduction. The complete loss of all Hh activity results in the most severe defects and the critical period for Hh activity is between 34 and 74 hours post fertilisation. Using a range of mutant genotypes that cause notochord and floorplate abnormalities, we show that the source of the Hh signals required for posterior gut formation is the endoderm and not the notochord, as previously postulated in mammalian models of ARMs. We show that Adriamycin, a drug known to cause ARMs in rat, but not chick embryos, has no effect on the development of the zebrafish gastrointestinal tract. These studies establish the zebrafish as a model for ARMs, and for the elucidation of other pathways involved in hindgut developmental processes.

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Section: Discussionmentioning

confidence: 99%

Section: Shha and Smo Mutant Fish Have Cloacal Abnormalitiesmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Hedgehog signalling is required for cloacal development in the zebrafish embryo

Parkin¹,

Allen²,

Ingham³

2009

Int. J. Dev. Biol.

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“…Neither of these neuronal sub-types express pax2a . gata3 is also expressed in the posterior pronephros and proctodeum (Thisse et al, 2004; www.zfin.org; Pyati et al, 2006;Wingert et al, 2007) and this may account for the slightly variable, relatively low-level expression of this gene in the trunk samples.…”

Section: Expression-profiling Using Affymetrix Microarraysmentioning

confidence: 99%

RNA profiling of FAC‐sorted neurons from the developing zebrafish spinal cord

Cerda

Hargrave

Lewis

2008

Developmental Dynamics

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In this report, we describe a successful protocol for isolating and expression-profiling live fluorescentprotein-labelled neurons from zebrafish embryos. As a proof-of-principle for this method, we FAC-sorted and RNA-profiled GFP-labelled spinal CiA interneurons and compared the expression profile of these cells to those of post-mitotic spinal neurons in general and to all trunk cells. We show that RNA of sufficient quality and quantity to uncover both expected and novel transcription profiles via Affymetrix microarray analysis can be extracted from 5,700 to 20,000 FAC-sorted cells. As part of this study, we also further confirm the genetic homology of mammalian and zebrafish V1 interneurons, by demonstrating that zebrafish V1 cells (CiAs) express genes that encode for the transcription factors Lhx1a and Lhx5. This protocol for dissociating, sorting and RNA-profiling neurons from organogenesis-stage zebrafish embryos should also be applicable to other developing organs and tissues and potentially other model organisms. Developmental Dynamics 238:150 -161, 2009.

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“…Separation of the lens from the cornea takes place as a result of cell death (as the lens forms, the nuclei of the lens fiber cells fragment their DNA and eventually disappear, giving rise to the clear lens fiber cells as a partial apoptosis) (Gao et al, 1997, Gupta et al, 2002. Other deaths are seen in the formation of olfactory pits (Pellier and Astic, 1994); in the formation of the cloaca in fish (Pyati et al, 2006); along the margins of the somites (Jeffs and Osmond, 1992, Sanders, 1997); and between the digits and in the axilla of developing limbs (Zuzarte-Luis and Hurle, 2002). Primarily because of ease of access, these tissues have received the most attention, but there are undoubtedly many other instances of cell death in the embryo.…”

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confidence: 99%

What cell death does in development

Zakeri¹,

Penaloza²,

Smith³

et al. 2015

Int. J. Dev. Biol.

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Cell death is prominent in gametogenesis and shapes and sculpts embryos. In nonmammalian embryos one sees little or no cell death prior to the maternal-zygotic transition, but, in mammalian embryos, characteristic deaths of one or two cells occur at the end of compaction and are apparently necessary for the separation of the trophoblast from the inner cell mass. Considerable sculpting of the embryo occurs by cell deaths during organogenesis, and appropriate cell numbers, especially in the CNS and in the immune system, are generated by massive overproduction of cells and selection of a few, with death of the rest. The timing, identity, and genetic control of specific cells that die have been well documented in Caenorhabditis, but in other embryos the stochastic nature of the deaths limit our ability to do more than identify the regions in which cells will die. Complete disruption of the cell death machinery can be lethal, but many mutations of the regulatory machinery yield only modest or no phenotypes, indicating substantial redundancy and compensation of regulatory mechanisms. Most of the deaths are apoptotic and are identified by techniques used to recognize apoptosis, but techniques identifying lysosomes (whether in dying or involuting cells or in the phagocytes that invade the tissue) also reveal patterns of cell death. Aberrant cell deaths that produce known phenotypes are typically localized, indicating that the mechanism of activating a programmed death in a specific region, rather than the mechanism of death, is aberrant. These results lead us to conclude that we need to know much more about the conversations among cells that lead cells to commit suicide. KEY WORDS: apoptosis, autophagy, caspase, necrosis, programmed cell death Cell death in development -a historical perspectiveCell death is a fundamental aspect of normal embryonic differentiation, morphogenesis, and teratogenesis, as well as of many diseases. Our understanding of these facts stems from considerable research as well as from old and very recent information. The great 19 th century embryologist, Carl Vogt, saw cell death in embryos as early as 1842 (Vogt, 1842), and by 1885 Walter Flemming documented the obvious, that loss of tadpole structures during metamorphosis resulted from cell death (Flemming, 1885). Throughout the early 20 th Century, many researchers examined the death of larval tissues in metamorphosing insects. By mid-century, Levi-Montalcini and Hamburger had demonstrated that the same number of neurons were born in each sympathetic or sensory ganglion, whether or not the ganglion supported a limb (Hamburger and Levi-Montalcini, 1949). Subsequently, in segments of the spinal cord that did not support a limb, many of the neurons died, resulting in much smaller ganglia (Hamburger and Levi-Montalcini, 1949). These investigations ultimately led to the discovery of nerve Int. J. Dev. Biol. 59: 11-22 (2015) doi: 10.1387/ijdb.150220zz Abbreviations used in this paper: CHX, cycloheximide; CNS, central nervous system; ICM, in...

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Sustained Bmp signaling is essential for cloaca development in zebrafish

Cited by 82 publications

References 39 publications

Hedgehog signalling is required for cloacal development in the zebrafish embryo

Hedgehog signalling is required for cloacal development in the zebrafish embryo

RNA profiling of FAC‐sorted neurons from the developing zebrafish spinal cord

What cell death does in development

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