Bmp‐2 downstream targets in mesenchymal development identified by subtractive cloning from recombinant mesenchymal progenitors (C3H10T½)

Abstract

A Bmp-dependent in vitro model was used to identify cDNAs during the manifestation of mesenchymal lineages. This model involves the recombinant expression of Bmps (Bmp-2, Bmp-4-7) in murine mesenchymal C3H10T 1 ⁄2 progenitors, which leads to the differentiation into three lineages: the osteogenic, the chondrogenic and the adipogenic lineage, albeit in varying efficiencies. By subtractive cloning, 21 Bmp-2-regulated cDNAs from C3H10T 1 ⁄2 mesenchymal progenitors were identified; 20 were related to known sequences and 1 was not. During mouse embryonic development, many of these cDNAs are expressed in chondrogenic, osteogenic, and in adipogenic tissues. Novel findings include a G 0 /G 1 switch gene (G0S2), which was demonstrated to be predominantly expressed in adipose tissue during late murine embryonic development. Furthermore, the membrane-standing glycoprotein autotaxin (ATX) is expressed, at precartilage condensations, joint regions, and during tooth development. An as yet undescribed cDNA, 29A, which encodes a putative secreted factor, is expressed in developing osteo-/chondrogenic tissues of vertebrae, ribs, tooth, and the limb bud. C3H10T 1 ⁄2-progenitors, therefore, may serve as a legitimate model for the investigation of the Bmp-mediated events during mesenchymal differentiation. Dev. Dyn. 1998;213:398-411.1998 Wiley-Liss, Inc.

The epigenetic reprogramming of poorly aggressive melanoma cells by a metastatic microenvironment

“…Coincident with the strong epigenetically induced expression of CD13 is a similar induction of the putative lymphocyte G0/G1 switch gene. Although this gene was originally thought to be the lectin-induced switch of lymphocytes from the G0 to the G1 phase of the cell cycle [46], more recent findings indicate its involvement in mesenchymal progenitor developmental events leading to the differentiation of osteogenic, chondrogenic and predominantly adipogenic lineages [47]. The strong induction of the putative lymphocyte G0/G1 switch gene in the poorly aggressive melanoma cells supports the hypothesis that these cells acquire a dedifferentiated, plastic phenotype, similar to that associated with the aggressive melanoma cells.…”

Transforming Growth Factor-β-activated Kinase-1 (TAK1), a MAP3K, Interacts with Smad Proteins and Interferes with Osteogenesis in Murine Mesenchymal Progenitors

“…The features of C3H10T 1 ⁄2-BMP2 cells have been described (28,29). Recombinant human BMP4 and TGF-␤1 were purchased from R&D Systems (Wiesbaden, Germany), and recombinant human IL-1␤ was from RELIATech GmbH (Braunschweig, Germany).…”

Expression patterns of the lysophospholipid receptor genes during mouse early development

“…Thus, LPA 1 signaling might be either a positive or a negative regulator of neural crest cell migration. Interestingly, it has been reported that BMP and Wnt signaling molecules lie downstream of autotaxin, an enzyme required for LPA biosynthesis (Bachner et al, 1998;Kenny et al, 2005).…”