Expression patterns of the lysophospholipid receptor genes during mouse early development

Abstract

Lysophospholipids (LPs) such as lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are known to mediate various biological responses, including cell proliferation, migration, and differentiation. To better understand the role of these lipids in mammalian early development, we applied whole-mount in situ hybridization techniques to E8.5 to E12.5 mouse embryos. We determined the expression patterns of the following LP receptor genes, which belong to the G protein-coupled receptor (GPCR) family: EDG1 to EDG8 (S1P 1 to S1P 5 and LPA 1 to LPA 3 ), LPA 4 (GPR23 /P2Y9), and LPA 5 (GPR92). We found that the S1P/LPA receptor genes exhibit overlapping expression patterns in a variety of organ primordia, including the developing brain and cardiovascular system, presomitic mesoderm and somites, branchial arches, and limb buds. These results suggest that multiple receptor systems for LPA/S1P lysophospholipids may be functioning during organogenesis. Developmental Dynamics 237:3280 -3294, 2008.

The lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) receptor gene families: cloning and comparative expression analysis in Xenopus laevis

“…The embryonic gene expression profile of the S1P genes has been reported in other vertebrate species, especially in the mouse in which a comparative analysis of their expression during development has been published allowing direct comparison with our study (Ohuchi et al 2008;Meng and Lee, 2009). Three members of S1P receptor family display specific and distinct expression profiles during frog embryogenesis.…”

“…During this study, a complementary, systematic study of these receptors during mouse development was published (Ohuchi et al 2008). Although some similarities can be drawn between these two studies, several differences in the expression profiles indicate potentially distinct roles that these receptors might have acquired during vertebrate evolution.…”

“…Although the sequences for several LPA/S1P receptors were available in databases, we cloned cDNAs for 8 of these receptors by in silico analysis, due to the increased coverage of ESTs and the X. tropicalis genomic DNA sequencing projects. In particular we identified sequences for X. tropicalis LPA3 and S1P3 that previous studies failed to identify (Lloyd et al 2005;Ohuchi et al 2008). Bioinformatics analysis indicated that all the sequences display the characteristic extracellular N terminus and the 7 transmembrane domains (TM) of these receptors which are located at the same position to the human orthologous sequences.…”

“…S1P3 expression is not regulated during embryogenesis and we failed to detect its expression by in situ hybridisation. In mouse and chick embryos, this receptor is expressed widely in non neuronal cells but null-S1P3 mice display no gross phenotype suggesting that this receptor is not required for normal development and function Ohuchi et al 2008;Meng and Lee, 2009). Our result shows that S1P4 is only weakly expressed during embryogenesis.…”

“…Our result shows that S1P4 is only weakly expressed during embryogenesis. In the mouse, S1P4 is first expressed ubiquitously, but its expression is not detected or is only at low levels after E12.5, suggesting that like in the frog, this gene might not play a major role during embryogenesis although expression in specific neural tissues might suggest a potential role during neural formation (Ohuchi et al 2008;Meng and Lee, 2009). No knock-out mice are available to confirm this hypothesis.…”