BMP‑2 and miR‑29c in osteosarcoma tissues on proliferation and invasion of osteosarcoma cells

Abstract

Expression of bone morphogenetic protein (BMP)-2 and microRNA (miR)-29c in osteosarcoma tissues and effects on proliferation and invasion of osteosarcoma cells were investigated. A retrospective analysis of 75 patients with osteosarcoma who underwent surgery in Tianjin Baodi Hospital from May 2013 to June 2017 was conducted. A total of 75 osteosarcoma tissues and 51 normal paraneoplastic tissues were collected. RT-PCR was used to compare the expression difference of BMP-2 and miR-29c. miR-29c mimics (experimental group A) and BMP-2 siRNA plasmid (experimental group B) were transfected into human osteosarcoma cells MG-63, respectively. The transfected cells were divided into miRNA negative control (miR-NC) group (cells transfected with miR-negative control), siRNA negative control group (cells transfected with non-silent siRNA) and blank group (cells without any transfection). MTT assay was used to detect cell proliferation in each group at different time periods. Transwell insert was used to detect invasion of cells in vitro . The relative expression of BMP-2 in osteosarcoma tissue was significantly higher than that in paraneoplastic tissue (P<0.05). Τhe relative expression of miR-29c in osteosarcoma tissue was significantly lower than that in paraneoplastic tissue (P<0.05). The cell survival rates in experimental groups A and B were significantly lower than those in the blank, miR-NC negative control and siRNA negative control groups on day 5 (P<0.05). The number of cell transmembranes in experimental groups A and B was significantly lower than those in the blank, miR-NC negative control and siRNA negative control groups (P<0.05). BMP-2 is over-expressed in osteosarcoma tissues, and miR-29c is under-expressed in osteosarcoma tissues. Interfering with the expression of BMP-2 and overexpression of miR-29c can inhibit the proliferation and invasion of osteosarcoma cells, indicating that BMP-2 and miR-29c may be involved in the regulation of proliferation and metastasis of osteosarcoma cells and could be used as new molecular target markers for the diagnosis and treatment of osteosarcoma.

Differences in Cell Death and BMP-2 Expression in Core Biopsy Specimens of Malignant Bone Tumors Given Hydrogen Peroxide Compared to Liquid Nitrogen

“…A study by [18] examined BMP-2 expression in osteosarcoma cells from 75 patients given liquid nitrogen. The study results show that BMP-2 is overexpressed in osteosarcoma tissue.…”

ATG16L1 is a Potential Prognostic Biomarker and Immune Signature for Osteosarcoma: A Study Based on Bulk RNA and Single-Cell RNA-Sequencing

“… 40 Advances in understanding of the genetic determinants of osteosarcoma can facilitate risk stratification of patients, which potentially can outperform the current pathological staging system. 41 Osteosarcoma cells are formed in the TME, which contains a complex mixture of other cells (MSCs, fibroblasts, osteoblasts, and myeloid immune cells such as CD8 + T cells). 42 This special TME environment is suitable for the development and metastasis of osteosarcoma.…”

KLF5‑induced miR‑487a augments the progression of osteosarcoma cells by targeting NKX3‑1 in vitro

“…MicroRNAs (miRNAs or miRs) are a class of single-stranded, non-coding small RNA that regulate gene expression by binding to the 3′ untranslated region (3′-UTR) of target genes ( 6 , 7 ). miRNAs have been reported to be associated with biological processes including cell proliferation, migration and inflammation, as well as apoptosis ( 8 , 9 ). In addition, miRNAs serve as oncogenes or cancer suppressors, which serves a key role in tumorigenesis ( 10 – 12 ).…”