Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota

Jiao, Xinyao; Wang, Yuehua; Yang, Lin; Lang, Yuxi; Li, Enhui; Zhang, Xiuyan; Zhang, Qi; Feng, Ying; Meng, Xianjun; Li, Bin

doi:10.1016/j.jnutbio.2018.07.008

Cited by 217 publications

(126 citation statements)

References 70 publications

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“…In this phylum, Akkermansia was an attractive bacterium that was first isolated from human feces (Dubourg et al, ). While relative abundance of Actinobacteria was markedly lowered by GPE and GSE administration compared to HFD + Abx +/− group, consisting with a previous research (Jiao et al, ).…”

Section: Discussionsupporting

confidence: 85%

Effects of grape pomace and seed polyphenol extracts on the recovery of gut microbiota after antibiotic treatment in high‐fat diet‐fed mice

Lü

Liu

Zhou

et al. 2019

Food Science & Nutrition

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The widespread use of antibiotics all over the world increases the risk of many metabolic diseases by altering the gut microbiota. Grape by‐products are of particular interest in the prevention of metabolic diseases, while only minimum amounts of these wastes are up‐graded or recycled at present. The study investigated the effect of grape pomace (GPE) and seed (GSE) polyphenol extracts on the recovery of gut microbiota after antibiotic cocktail treatment in high‐fat diet‐fed (HFD) mice. C57BL/6J mice were fed HFD together with antibiotic treatment by drinking water for 3 weeks. Then, GPE and GSE were supplemented for 7 days after withdrawal of antibiotics. The gut microbiota was characterized by a significant loss of diversity and certain important taxon after a 3‐week antibiotic cocktail treatment. The GPE and GSE supplementation during the restore period of gut microbiota had some positive effects. The relative abundance of gut microbiota was improved by GPE and GSE compared to the spontaneous recovery group. And gut microbiota diversity was also greatly changed by GPE and GSE, being indicated by the changes of Verrucomicrobia and Akkermansia in feces. These findings suggested that grape polyphenol extracts have a great influence on the recovery of gut microbiota after antibiotics and high‐fat diet treatment.

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Section: Discussionsupporting

confidence: 85%

Effects of grape pomace and seed polyphenol extracts on the recovery of gut microbiota after antibiotic treatment in high‐fat diet‐fed mice

Lü

Liu

Zhou

et al. 2019

Food Science & Nutrition

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“…Our Evidence-Based Complementary and Alternative Medicine study confirmed the positive effects of orlistat on weight loss and glucose tolerance. Although a previous study by Jiao et al demonstrated the antiobesity effect of blueberry polyphenols extract by modulation of gut microbiota, utilized orlistat as control group, and focused on lipid metabolism [25], in our study, we examined the effect of orlistat, a classic anti-obesity medication, on metabolic alleviation, in particularly, in impaired glucose metabolism, and gut microbiota, which was analyzed more comprehensively. Our results showed that HFD consumption caused significantly decreased microbial diversity and richness, and orlistat supplementation further reduced it.…”

Section: Discussionmentioning

confidence: 99%

“…Our results showed that HFD consumption caused significantly decreased microbial diversity and richness, and orlistat supplementation further reduced it. Several studies investigating the antiobesity effects of natural active ingredients, such as catechin and blueberry polyphenols extract [25,26], observed the modulation of the gut microbiota composition with downregulation of the microbial diversity. Similar results on orlistat were also documented [25].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies investigating the antiobesity effects of natural active ingredients, such as catechin and blueberry polyphenols extract [25,26], observed the modulation of the gut microbiota composition with downregulation of the microbial diversity. Similar results on orlistat were also documented [25]. Moreover, a previous study by Collins et al showed a decrease in observed species in HFD fed mice compared to a low-fat group; reversion of such changes was not found in group supplemented with polyphenols [27].…”

Section: Discussionmentioning

confidence: 99%

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Orlistat-Induced Gut Microbiota Modification in Obese Mice

Cao

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Introduction. Accumulating evidence has indicated that alterations of gut microbiota have been involved in various metabolic diseases. Orlistat, a reversible inhibitor of pancreatic and gastric lipase, has beneficial effects on weight loss and metabolism. However, the effect of orlistat on the composition of gut microbiota remains unclear. Objective. We aimed to explore the effect of orlistat on gut microbiota in high-fat diet (HFD) fed C57BL/6J obese mice. Methods. C57BL/6J mice were randomly divided into three groups: control (NCD), HFD, and HFD + orlistat (ORL). Mice in the NCD group were fed chow diet, while the other groups were fed HFD for 6 months, and orlistat was added in the final 3 months in the HFD + ORL group. After sacrifice, body weight and metabolic parameters were assessed, and the gut microbial composition was analyzed by 16S rRNA gene sequencing. Results. Orlistat treatment exerted beneficial effects on body weight, plasma cholesterol, and glucose tolerance. Meanwhile, orlistat treatment modified the gut microbiota, presenting as reduced total microbial abundance and obvious upregulated bacteria. Moreover, the upregulated bacteria correlated with several metabolic pathways. Conclusions. Orlistat may exert beneficial effects on body weight and glucose tolerance through modifying the composition of gut microbiota.

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“…Among the life style factors affecting gut microbial diversity, diet quality is of paramount importance and contribute significantly to the determination of gut microbiota composition 16 . In this context, berry polyphenols, including wild blueberry, have recently been shown to significantly promote the abundance of beneficial bacteria involved in the attenuation of obesity-associated disorders and dysbiosis 17,18 . Indeed, in rats fed a high-fat diet, the supplementation of blueberry powder for 8-weeks improved insulin sensitivity and systemic inflammation in association with major changes in gut microbiota composition 19 .…”

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confidence: 99%

Wild blueberry proanthocyanidins shape distinct gut microbiota profile and influence glucose homeostasis and intestinal phenotypes in high-fat high-sucrose fed mice

Rodríguez-Daza

Daoust

Boutkrabt

et al. 2020

Sci Rep

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Blueberries are a rich source of polyphenols, widely studied for the prevention or attenuation of metabolic diseases. However, the health contribution and mechanisms of action of polyphenols depend on their type and structure. Here, we evaluated the effects of a wild blueberry polyphenolic extract (WBe) (Vaccinium angustifolium Aiton) on cardiometabolic parameters, gut microbiota composition and gut epithelium histology of high-fat high-sucrose (HfHS) diet-induced obese mice and determined which constitutive polyphenolic fractions (BPF) was responsible for the observed effects. To do so, the whole extract was separated in three fractions, F1) Anthocyanins and phenolic acids, F2) oligomeric proanthocyanidins (PACs), phenolic acids and flavonols (PACs degree of polymerization DP < 4), and F3) PACs polymers (PACs DP > 4) and supplied at their respective concentration in the whole extract. After 8 weeks, WBE reduced OGTT AUC by 18.3% compared to the HFHS treated rodents and the F3 fraction contributed the most to this effect. The anthocyanin rich F1 fraction did not reproduce this response. WBE and the BPF restored the colonic mucus layer. Particularly, the polymeric PACs-rich F3 fraction increased the mucin-secreting goblet cells number. WBE caused a significant 2-fold higher proportion of Adlercreutzia equolifaciens whereas oligomeric PACs-rich F2 fraction increased by 2.5-fold the proportion of Akkermansia muciniphila. This study reveals the key role of WBE PACs in modulating the gut microbiota and restoring colonic epithelial mucus layer, providing a suitable ecological niche for mucosa-associated symbiotic bacteria, which may be crucial in triggering health effects of blueberry polyphenols.Wild blueberries (Vaccinium angustifolium Aiton) are recognized as a rich source of bioactive phenolic compounds, the consumption of which has been associated with the attenuation of metabolic disorders through its beneficial action on glucose homeostasis and the reduction the oxidative stress and intestinal inflammation 1,2 . Until now, these positive effects have predominantly been attributed to flavonoids and in particular to anthocyanins 3,4 . However, wild blueberry has a complex profile of bioactive polyphenols; in addition to anthocyanins, it contains chlorogenic acid, and oligomers and polymers of flavan-3-ols, such as proanthocyanidins (PACs) 5 . Interestingly, there are increased evidences showing that PACs can attenuate the progression of metabolic syndrome, including type 2 diabetes and obesity 6 . Pre-clinical studies have suggested different mechanisms by Figure 3. Wild blueberry polyphenolic fractions restore the mucus thickness and polymeric PACs-rich fraction significantly stimulated mucin-secreting goblet cells number in HFHS-fed mouse colon. The effect of HFHS-diet and the supplementation with WBE and BPF: anthocyanin and phenolic acids (F1), oligomeric PACs, phenolic acids and flavonols (F2) and polymeric PACs (F3) were studied in mouse colon morphology. A combination of Alcian Blue and periodic acid-Schiff...

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Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota

Cited by 217 publications

References 70 publications

Effects of grape pomace and seed polyphenol extracts on the recovery of gut microbiota after antibiotic treatment in high‐fat diet‐fed mice

Effects of grape pomace and seed polyphenol extracts on the recovery of gut microbiota after antibiotic treatment in high‐fat diet‐fed mice

Orlistat-Induced Gut Microbiota Modification in Obese Mice

Wild blueberry proanthocyanidins shape distinct gut microbiota profile and influence glucose homeostasis and intestinal phenotypes in high-fat high-sucrose fed mice

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