Blueberry (Vaccinium virgatum) Leaf Extracts Protect Against Aβ-Induced Cytotoxicity and Cognitive Impairment

Abstract

The ethylacetate (EtOAc) fraction of blueberry leaf extract was investigated to examine the in vivo antiamnesic effects against amyloid β protein (Aβ)-induced learning and memory deficit. The fraction showed the highest antioxidant activities, and the generation of intracellular reactive oxygen species was significantly decreased. Cell viability assays revealed the in vitro cytoprotective effects of the fraction, and the cytoplasmic lactate dehydrogenase release into the medium was dose-dependently inhibited. In addition, a chlorogenic acid was identified as a predominant phenolic compound by high-performance liquid chromatography analysis. Antiamnesic effects were evaluated by using in vivo the Y-maze and passive avoidance tests, and preadministration of the fraction attenuated Aβ-induced memory impairment in both in vivo experiments. Acetylcholinesterase prepared from mice brain was inhibited by the fraction, and malondialdehyde generation in the brain homogenate was also decreased. These findings suggest that the EtOAc fraction of blueberry leaf extract could possess a wide range of physiological effects against neurodegenerative diseases.

Effect of anthocyanins from rabbit-eye blueberry (Vaccinium virgatum) on cognitive function in mice under trimethyltin-induced neurotoxicity

“…Lipid peroxidation can alter the fluidity of the plasma membrane, and the LDH assay provides an estimation of the percentage of surviving PC12 cells (18). The anthocyanin fraction protected the integrity of the cellular membrane at all tested concentrations (Fig.…”

“…Preparation of brain tissue After in vivo behavioral tests, brain tissues of mice and homogenates were prepared according to the method of Jeong et al (18). Measurement of malondialdehyde (MDA) levels in brain homogenates MDA levels in brain tissues were examined according to the method of Jeong et al (18).…”

“…Measurement of malondialdehyde (MDA) levels in brain homogenates MDA levels in brain tissues were examined according to the method of Jeong et al (18). The MDA level was assayed for products of lipid peroxidation based on monitoring of thiobarbituric acid reactive substance formation (17).…”

Promising Plant Sources of Anti-Diabetic Micronutrients

“…In particular, consumption of the ethyl acetate fraction of bilberry leaf extract (Vaccinium virgatum) in a dose of 20 mg/kg of the body weight by ICR mice had antiamnesic effects, which, according to the authors of [87], supports the potential benefit of its use for neurodegenerative diseases. As is evidenced by [88], obesity caused by consumption of high-fat foods by C57BL/6 mice for a period of 10 weeks.…”

Cognitive Enhancers (Nootropics). Part 3: Drugs Interacting with Targets other than Receptors or Enzymes. Disease-Modifying Drugs. Update 2014

“…Blueberry (Vaccinium virgatum) leaf extracts protected against A␤-induced cytotoxicity and cognitive impairment [167].…”