Blue light irradiation triggers the antimicrobial potential of ZnO nanoparticles on drug-resistant Acinetobacter baumannii

Yang, Ming-Yeh; Chang, Kai‐Chih; Chen, Liang-Yü; Wang, Po-Ching; Chou, C. Perry; Wu, Zhong-Bin; Hu, Anren

doi:10.1016/j.jphotobiol.2018.02.003

Cited by 29 publications

(15 citation statements)

References 44 publications

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“…The significant bactericidal efficacy of both aBL and aPDI against A. baumannii was repeatedly reported in numerous published in vitro and in vivo studies (Dai et al, 2009; Huang et al, 2014; Maisch et al, 2014; Zhang et al, 2014; Yuan et al, 2017; Yang et al, 2018). However, the first published evidence of a combined aPDI/antibiotic approach being used against pandrug-resistant A. baumannii was presented by Boluki et al (2017).…”

Section: Discussionmentioning

confidence: 63%

“…A lack of effective antimicrobials has forced the need for the development of novel strategies to control A. baumannii infections. One of these approaches is antimicrobial photodynamic inactivation (aPDI) or antimicrobial blue light (aBL) (Nitzan et al, 1998; Dai et al, 2009; Cai et al, 2012; Huang et al, 2014; Yuan et al, 2017; Yang et al, 2018). These strategies exert high bactericidal efficacy toward various microbes regardless of antibiotic resistance.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobials Are a Photodynamic Inactivation Adjuvant for the Eradication of Extensively Drug-Resistant Acinetobacter baumannii

et al. 2019

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The worldwide emergence of extensively drug resistant (XDR) Acinetobacter baumannii has reduced the number of antimicrobials that exert high bactericidal activity against this pathogen. This is the reason why many scientists are focusing on investigations concerning novel non-antibiotic strategies such as antimicrobial photodynamic inactivation (aPDI) or the use of antimicrobial blue light (aBL). Therefore, the aim of the current study was to screen for antimicrobial synergies of routinely used antibiotics and phototherapies, including both aPDI involving exogenously administered photosensitizing molecules, namely, rose bengal, and aBL, involving excitation of endogenously produced photoactive compounds. The synergy testing was performed in accordance with antimicrobial susceptibility testing (AST) standards, including various methodological approaches, i.e., antibiotic diffusion tests, checkerboard assays, CFU counting and the evaluation of postantibiotic effects (PAEs). We report that combining antimicrobials and aPDI/aBL treatment led to a new strategy that overcomes drug resistance in XDR A. baumannii , rendering this pathogen susceptible to various categories of antibiotics. Sublethal aPDI/aBL treatment in the presence of sub-MIC levels of antimicrobials effectively killed A. baumannii expressing drug resistance to studied antibiotics when treated with only antibiotic therapy. The susceptibility of XDR A. baumannii to a range of antibiotics was enhanced following sublethal aPDI/aBL. Furthermore, 3′-( p -aminophenyl) fluorescein (APF) testing indicated that significantly increased reactive oxygen species production upon combined treatment could explain the observed synergistic activity. This result represents a conclusive example of the synergistic activity between photodynamic inactivation and clinically used antimicrobials leading to effective eradication of XDR A. baumannii isolates and indicates a potent novel therapeutic approach.

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Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Antimicrobials Are a Photodynamic Inactivation Adjuvant for the Eradication of Extensively Drug-Resistant Acinetobacter baumannii

et al. 2019

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“…CA could utilize these mechanisms in PDT, which would result in cancer cell damage [26]. Previously, Yang et al reported the cytotoxic action of CA through aerobic photo-oxidation upon irradiation with blue light [27]. Numerous research evidence refers to the health-promoting capability of CAs with reference to its antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Phthalocyanine Mediated Photodynamic Therapy by Catechin on Lung Cancer Cells

2020

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Worldwide, lung cancer remains one of the leading cancers with increasing mortality rates. Though chemotherapy for lung cancer is effective, it is always accompanied by unavoidable and grave side effects. Photodynamic therapy (PDT), using novel photosensitizers, is an advanced treatment method with relatively few side effects. Plant products are emerging as potent photosensitizers (PSs). The dose-dependent effect of Catechin (CA) (20–100 µM) on cellular morphological changes, cell viability, cytotoxicity, proliferation, DNA damage and apoptosis were studied on A549 adenocarcinoma alveolar basal epithelial cells. The effect of CA, along with Zinc phthalocyanine PS at 680 nm and 5 J/cm2 fluency was also studied. As the doses of CA increased, the results showed a pattern of increased cytotoxicity, accompanied by decreased cell viability and proliferation in A549 cells. Also, at 52 µM (IC50), CA in combination with PS significantly increased the cytotoxicity, DNA damage, and apoptosis, as compared to control and PS alone, treated cells in PDT experiments. These findings leave a possible thread that CA can be used in the application of phyto-photodynamic therapy of cancer in future.

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“…An excited PS can transfer electrons or energy to biomolecules or molecular oxygen, resulting in the formation of reactive oxygen species (ROS) or singlet oxygen radicals, which are toxic to cellular targets such as nucleic acids, proteins and lipids (Mai et al, 2017; Yang M.-Y. et al, 2018). Some of the most frequently used PSs include phenothiazinium derivatives (methylene blue, toluidine blue), xanthine derivatives (rose bengal), porphyrin, chlorin, or fullerene derivatives amongst many others (Abrahamse and Hamblin, 2016; Cieplik et al, 2018).…”

Section: Photodynamic Light Therapymentioning

confidence: 99%

Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review

et al. 2019

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The acronym ESKAPE includes six nosocomial pathogens that exhibit multidrug resistance and virulence: Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa , and Enterobacter spp. Persistent use of antibiotics has provoked the emergence of multidrug resistant (MDR) and extensively drug resistant (XDR) bacteria, which render even the most effective drugs ineffective. Extended spectrum β-lactamase (ESBL) and carbapenemase producing Gram negative bacteria have emerged as an important therapeutic challenge. Development of novel therapeutics to treat drug resistant infections, especially those caused by ESKAPE pathogens is the need of the hour. Alternative therapies such as use of antibiotics in combination or with adjuvants, bacteriophages, antimicrobial peptides, nanoparticles, and photodynamic light therapy are widely reported. Many reviews published till date describe these therapies with respect to the various agents used, their dosage details and mechanism of action against MDR pathogens but very few have focused specifically on ESKAPE. The objective of this review is to describe the alternative therapies reported to treat ESKAPE infections, their advantages and limitations, potential application in vivo , and status in clinical trials. The review further highlights the importance of a combinatorial approach, wherein two or more therapies are used in combination in order to overcome their individual limitations, additional studies on which are warranted, before translating them into clinical practice. These advances could possibly give an alternate solution or extend the lifetime of current antimicrobials.

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Blue light irradiation triggers the antimicrobial potential of ZnO nanoparticles on drug-resistant Acinetobacter baumannii

Cited by 29 publications

References 44 publications

Antimicrobials Are a Photodynamic Inactivation Adjuvant for the Eradication of Extensively Drug-Resistant Acinetobacter baumannii

Antimicrobials Are a Photodynamic Inactivation Adjuvant for the Eradication of Extensively Drug-Resistant Acinetobacter baumannii

Enhancement of Phthalocyanine Mediated Photodynamic Therapy by Catechin on Lung Cancer Cells

Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review

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