Blue Light–Induced Oxidative Stress in Human Corneal Epithelial Cells: Protective Effects of Ethanol Extracts of Various Medicinal Plant Mixtures

Lee, Jee-Bum; Kim, Soohyun; Lee, Seung−Chul; Kim, Hee-Gu; Ahn, Ho-Geun; Li, Zhengri; Yoon, Kyung Chul

doi:10.1167/iovs.13-13441

Cited by 64 publications

(78 citation statements)

References 45 publications

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“…Accumulating evidence suggests that oxidative stress has an important role in ocular surface diseases, including DED. 40,57,58 In vivo lipid oxidative injury has been observed in DED and Sjögren's syndrome patients. 59,60 In vitro studies further revealed that lipid and mitochondrial oxidative damage causes inflammation in HCE cells.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Lee

Choi

Cui

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Citation: Lee HS, Choi J-H, Cui L, et al. Anti-inflammatory and antioxidative effects of Camellia japonica on human corneal epithelial cells and experimental dry eye: in vivo and in vitro study. Invest Ophthalmol Vis Sci. 2017;58:119658: -120758: . DOI:10.1167 PURPOSE. To analyze the anti-inflammatory and antioxidative effects of Camellia japonica (CJ) on human corneal epithelial (HCE) cells and its therapeutic effects in a mouse model of experimental dry eye (EDE). METHODS.Camellia japonica extracts of varying concentrations (0.001%, 0.01%, and 0.1%) were used to treat HCE cells. Dichlorofluorescein diacetate (DCF-DA) and dihydroethidium (DHE) assays were performed. The production of peroxiredoxin (PRX) 1-6 and manganesedependent superoxide dismutase (MnSOD) in HCE cells was assessed using Western blot analysis. Furthermore, eye drops containing 0.001%, 0.01%, or 0.1% CJ extract or a balanced salt solution (BSS) were applied to the EDE. Clinical parameters were measured 7 days after treatment. The levels of inflammatory markers and intracellular reactive oxygen species (ROS) were measured.RESULTS. Treatment with 0.01% and 0.1% CJ extracts decreased apoptosis in HCE cells. In addition, band intensities of PRX 1, 4, and 5, as well as MnSOD, after hydrogen peroxide (H 2 O 2 ) treatment showed a significant improvement after pretreatment with 0.01% and 0.1% CJ extracts. Mice treated with 0.1% CJ extract showed significantly improved clinical parameters when compared to those of the EDE control and BSS groups. A significant decrease in the levels of inflammatory markers and intracellular ROS was observed in the 0.01% and 0.1% CJ extract groups.CONCLUSIONS. Camellia japonica extracts promoted antioxidative protein expression and suppressed apoptosis in HCE cells. Furthermore, CJ extracts improved clinical signs of dry eye and reduced oxidative stress and the expression of inflammatory markers, suggesting that eye drops containing CJ extract could be used as an adjunctive treatment for dry eye.

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Lee

Choi

Cui

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…Lee et al investigated if blue light affects human corneal epithelial cells (Lee et al, 2014). Cells were seeded in 35-mm tissue culture dishes and were exposed to 410 or 480 nm blue light.…”

Section: Effect Of Antimicrobial Blue Light On Host Cells and Tissuesmentioning

confidence: 99%

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Wang

et al. 2017

Drug Resistance Updates

208

192

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As an innovative non-antibiotic approach, antimicrobial blue light in the spectrum of 400–470 nm has demonstrated its intrinsic antimicrobial properties resulting from the presence of endogenous photosensitizing chromophores in pathogenic microbes and, subsequently, its promise as a counteracter of antibiotic resistance. Since we published our last review of antimicrobial blue light in 2012, there have been a substantial number of new studies reported in this area. Here we provide an updated overview of the findings from the new studies over the past 5 years, including the efficacy of antimicrobial blue light inactivation of different microbes, its mechanism of action, synergism of antimicrobial blue light with other angents, its effect on host cells and tissues, the potential development of resistance to antimicrobial blue light by microbes, and a novel interstitial delivery approach of antimicrobial blue light. The potential new applications of antimicrobial blue light are also discussed.

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“…Blue light is an underestimated threat leading to elevated ROS in epithelial tissue [13]. Although cosmetic formulations exist effectively shielding UV-A and UV-B from the skin, a blue light filter may be hard to develop.…”

Section: Discussionmentioning

confidence: 99%

Blue Light Protecting Cosmetic Active Ingredients: A Case Report

Hettwer¹

2017

JDC

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Background: High energy visible lights more and more attracts notice in the cosmetic science field. It is well known that UV light harms skin cells and unprotected exposure to this harmful radiation leads to premature skin ageing. Recently, the impact of blue light on human skin is investigated in-vitro and in-vivo. Evidence grows that blue light increases reactive oxygen species (ROS) intracellular which leads to cell damage and subsequent apoptosis. Methods:HaCaT cells were cultivated and incubated with anti-oxidative cosmetic active ingredients. After irradiation of the cells with defined blue light, the intracellular ROS were detected by fluorescent staining and cell sorting. The effect of one cosmetic active ingredient was determined in an application study for three months, visualization of rosacea teleangiectoides and quantification of red spots was done with a VISIA-CR photographic system. Results:All investigated cosmetic active ingredients exhibited ROS reduction close to the baseline level after 2 hours of blue light irradiation. Application of one cosmetic active ingredient to the facial skin revealed a statistically significant reduction in red spots count and a markedly reduction of rosacea teleangiectoides by 44.3 % in one test subject. Conclusion:Anti-oxidant cosmetic active ingredients are capable of protecting skin cells from the impact of blue light and lead to a visible reduction of ROS induced skin aberrations in vivo.

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Blue Light–Induced Oxidative Stress in Human Corneal Epithelial Cells: Protective Effects of Ethanol Extracts of Various Medicinal Plant Mixtures

Abstract: Overexposure to blue light (410-480 nm) may have a harmful effect on human corneal epithelial cells compared with other visible light wavelengths. Medicinal plant extracts can have potent protective effects on blue light-induced oxidative stress.

Cited by 64 publications

References 45 publications

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Blue Light Protecting Cosmetic Active Ingredients: A Case Report

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