Blue light enhances astaxanthin biosynthesis metabolism and extraction efficiency in Haematococcus pluvialis by inducing haematocyst germination

Ma, Ruijuan; Thomas‐Hall, Skye R.; Chua, Elvis T.; Eltanahy, Eladl; Netzel, M.; Netzel, Gabriele; Lu, Yinghua; Schenk, Peer M.

doi:10.1016/j.algal.2018.08.023

Cited by 45 publications

(23 citation statements)

References 42 publications

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“…Therefore, understanding the stress‐induced lipid biosynthetic pathways is pivotal for the improvement in the production of both oil and astaxanthin in H. pluvialis . DGAT catalyzes the terminal step in acyl‐CoA‐dependent TAG production, and the expression of four DGAT2 isoforms was found to be upregulated in H. pluvialis under stress conditions (Ma et al, , b), which suggests their possible involvement in stress‐induced TAG accumulation. In the current study, we aim to identify the DGAT2 cDNA from the green algae H. pluvialis and functionally characterize the encoded enzymes using a yeast system.…”

Section: Discussionmentioning

confidence: 99%

“…In H. pluvialis , the fatty acid composition of total lipids and TAG was changed when the algae cells were subjected to light stress, in which 16:0, 18:1, and 18:2 increased with the concomitant decreases in 18:3 (Bilbao et al, ; Chen et al, ). Considering the upregulation of all four HpDGAT2 transcripts under light stress (Ma et al, , b), it is possible that different HpDGAT2 isoforms may display different substrate preferences and thus contribute to altered algal fatty acid composition. Indeed, C. reinhardtii DGAT2 have been demonstrated to contribute to the synthesis of diverse TAG species in algal cells by displaying distinct specificities toward acyl‐CoA and DAG (Liu et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme

Nguyen

Abdel-Hameed

et al. 2019

Lipids

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Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high-value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol acyltransferase (DGAT) catalyzes the last step of the acyl-CoA-dependent TAG biosynthesis and appears to represent a bottleneck in algal TAG formation. In this study, putative H. pluvialis DGAT2 cDNA (HpDGAT2A, B, D and E) were identified from a transcriptome database and were subjected to sequence-based in silico analyses. The coding sequences of HpDGAT2B, D, and E were then isolated and characterized through heterologous expression in a TAG-deficient Saccharomyces cerevisiae strain H1246. The expression of HpDGAT2D allowed the recovery of TAG biosynthesis in this yeast mutant, and further in vitro enzymatic assays confirmed that the recombinant HpDGAT2D possessed strong DGAT activity. Interestingly, the recombinant HpDGAT2D displayed sigmoidal kinetics in response to increasing acyl-CoA concentrations, which has not been reported in plant or algal DGAT2 in previous studies. Keywords Algae Á DGAT Á Haematococcus pluvialis Á Saccharomyces cerevisiae Á Sigmoidal kinetics Á Triacylglycerol biosynthesis Lipids (2020) 55: 425-433. Abbreviations DAG diacylglycerol DGAT diacylglycerol acyltransferase ER endoplasmic reticulum GC gas chromatography TAG triacylglycerol TLC thin layer chromatography * Guanqun Chen

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme

Nguyen

Abdel-Hameed

et al. 2019

Lipids

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“…The light quality was also shown to impact biomass productivity and astaxanthin accumulation. For example, it was reported that red light from Light Emitting Diodes (LED) increased the cell growth of H. pluvialis, while blue light enhanced astaxanthin biosynthesis [210].…”

Section: Irradiance Temperature and Ph Of The Mediamentioning

confidence: 99%

Novel Insights into the Biotechnological Production of Haematococcus pluvialis-Derived Astaxanthin: Advances and Key Challenges to Allow Its Industrial Use as Novel Food Ingredient

Jannel

Caro

Bermudes³

et al. 2020

JMSE

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Astaxanthin shows many biological activities. It has acquired a high economic potential and its current market is dominated by its synthetic form. However, due to the increase of the health and environmental concerns from consumers, natural forms are now preferred for human consumption. Haematococcus pluvialis is artificially cultured at an industrial scale to produce astaxanthin used as a dietary supplement. However, due to the high cost of its cultivation and its relatively low biomass and pigment productivities, the astaxanthin extracted from this microalga remains expensive and this has probably the consequence of slowing down its economic development in the lower added-value market such as food ingredient. In this review, we first aim to provide an overview of the chemical and biochemical properties of astaxanthin, as well as of its natural sources. We discuss its bioavailability, metabolism, and biological activities. We present a state-of-the-art of the biology and physiology of H. pluvialis, and highlight novel insights into the biotechnological processes which allow optimizing the biomass and astaxanthin productivities. We are trying to identify some lines of research that would improve the industrial sustainability and economic viability of this bio-production and to broaden the commercial potential of astaxanthin produced from H. pluvialis.

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“…reported that green light resulted in high growth rate of algae while the synthesis of phycocyanin and phycoerythrin can be stimulated by blue light . Additionally, in the cells of Haematococcus pluvialis , blue light promoted the accumulation of astaxanthin, a value‐added antioxidant for human diet . (v) Genetic modification technology is widely applied to enhance the synthesis of certain compositions, particularly protein and lipid, in microalgae .…”

Section: Microalgae Biotechnology For Blssmentioning

confidence: 99%

“…56 Additionally, in the cells of Haematococcus pluvialis, blue light promoted the accumulation of astaxanthin, a value-added antioxidant for human diet. 57 (v) Genetic modification technology is widely applied to enhance the synthesis of certain compositions, particularly protein and lipid, in microalgae. 58,59 Molecular genetic tools that have been used for genetic modification of algae include transformation methods, mutagenesis and breeding, and modulation of the endogenous genome using CRISPR systems.…”

Section: Cultivation Conditions Optimizationmentioning

confidence: 99%

Microalgae biotechnology as an attempt for bioregenerative life support systems: problems and prospects

Yang

Liu

et al. 2019

J of Chemical Tech & Biotech

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Bioregenerative life support system (BLSS) plays a key role in the long‐duration manned space mission. Microalgae‐based BLSS was proposed decades ago but it had a variety of technical problems hindering its application in space mission. However, in recent years, a lot of effort has been devoted to this field and great progresses have been achieved in the research of microalgae‐based BLSS. Therefore, the main aim of this work is to summarize the previous progress and find the challenges faced by microalgae‐based BLSS technology. In this regard, this works introduces the concept of microalgae‐based BLSS and presents its development paths in the past decades. Additionally, technology updates in the aspects of microalgae‐based oxygen generation, value‐added biomass production as food alternative, and wastes treatment by microalgae are summarized. Challenges faced by microalgae‐based BLSS and potential solutions are discussed. This work provides a deep understanding of microalgae‐based BLSS and that it would be beneficial for promoting implementation of this novel technology in manned space missions. © 2019 Society of Chemical Industry

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Blue light enhances astaxanthin biosynthesis metabolism and extraction efficiency in Haematococcus pluvialis by inducing haematocyst germination

Cited by 45 publications

References 42 publications

Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme

Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme

Novel Insights into the Biotechnological Production of Haematococcus pluvialis-Derived Astaxanthin: Advances and Key Challenges to Allow Its Industrial Use as Novel Food Ingredient

Microalgae biotechnology as an attempt for bioregenerative life support systems: problems and prospects

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