Blood Storage and the Rate of Chromosome Aberrations after in Vitro Exposure to Ionizing Radiations

Ivanov, B.; Léonard, A.; Deknudt, Gh.

doi:10.2307/3573852

Cited by 18 publications

(3 citation statements)

References 5 publications

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“…The ex vivo system proposed in this study, which uses whole blood, has never been applied until now in C. caretta or in other reptiles; the few studies concern humans [ 119 , 120 ] and monkeys [ 121 ]. The study by Ivanov et al [ 119 ] proved the ability of the ex vivo system to respond to ionizing radiations with formation of chromosome aberrations, a genotoxicity endpoint, while the studies by Fischer et al [ 120 ] and by Krakauer et al [ 121 ] demonstrated the modulation of cytokines secretion in whole blood after exposure to different compounds. Cocci et al [ 20 ] used erythrocyte cultures from C. caretta exposed to a phthalate, the DiDP.…”

Section: Resultsmentioning

confidence: 99%

A Novel Ex Vivo Approach Based on Proteomics and Biomarkers to Evaluate the Effects of Chrysene, MEHP, and PBDE-47 on Loggerhead Sea Turtles (Caretta caretta)

Bianchi

Casini

Vantaggiato

et al. 2022

IJERPH

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The principal aim of the present study was to develop and apply novel ex vivo tests as an alternative to cell cultures able to evaluate the possible effects of emerging and legacy contaminants in Caretta caretta. To this end, we performed ex vivo experiments on non-invasively collected whole-blood and skin-biopsy slices treated with chrysene, MEHP, or PBDE-47. Blood samples were tested by oxidative stress (TAS), immune system (respiratory burst, lysozyme, and complement system), and genotoxicity (ENA assay) biomarkers, and genotoxic and immune system effects were observed. Skin slices were analyzed by applying a 2D-PAGE/MS proteomic approach, and specific contaminant signatures were delineated on the skin proteomic profile. These reflect biochemical effects induced by each treatment and allowed to identify glutathione S-transferase P, peptidyl-prolyl cis-trans isomerase A, mimecan, and protein S100-A6 as potential biomarkers of the health-threatening impact the texted toxicants have on C. caretta. Obtained results confirm the suitability of the ex vivo system and indicate the potential risk the loggerhead sea turtle is undergoing in the natural environment. In conclusion, this work proved the relevance that the applied ex vivo models may have in testing the toxicity of other compounds and mixtures and in biomarker discovery.

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Section: Resultsmentioning

confidence: 99%

A Novel Ex Vivo Approach Based on Proteomics and Biomarkers to Evaluate the Effects of Chrysene, MEHP, and PBDE-47 on Loggerhead Sea Turtles (Caretta caretta)

Bianchi

Casini

Vantaggiato

et al. 2022

IJERPH

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“…The time from collection of the blood to the addition of phytohaemagglutinin (PHA) was no longer than 23 hours. Attention has been drawn recently to the influence of time and temperature of storage of blood in radiation-induced abnormalities in cultured lymphocytes (Ivanov et al, 1973). Culture proceeded for 48 hours at 37OC in 5% CO, in air.…”

Section: Methodsmentioning

confidence: 99%

Dose Response Relationships Beween Chromosome Damage and Absorbed Radiation

Chee

Ilbery

1975

Australas Radiol

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SUMMARY Chromosome aberration analysis provides a biological method of assessing absorbed radiation dose. This paper describes the types of aberration brought about in the chromosomes of lymphocytes by radiation‐induced chromosomal breaks, how they can be displayed and related to IN VITRO radiation exposure of blood lymphocyte cultures. A calibration chart incorporating these IN VITRO dose response relationships enables the IN VIVO exposure of individuals during radiotherapy to be compared with the dose giving the same number of chromosome aberrations brought about by IN VITRO irradiation of blood lymphocytes. Radiotherapeutic exposure in its scientifically defined exactness to known volumes of different body regions enables the accumulation of information invaluable to the determination of the amount of radiation received in those accidentally exposed.

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“…Delayed PHA stimulation and culture initiation of irradiated lymphocytes have been reported to affect the frequencies of radiation-induced chromosomal aberrations. 11,13,[15][16][17][18][19][20][21] Frequencies of dicentric and ring chromosomes were analyzed 2 in ex vivo irradiated blood samples after storage for 0 to 72 hours in the presence of varying concentrations of bromodeoxyuridine (BrdU; 9.7 µM, 19.4 µM, and 38.7 µM). The results showed a significant reduction in the aberration frequencies in the blood samples stored for 66 hours in the presence of 19.4 µM BrdU.…”

Section: Comparison Of Ionizing Radiation Induced Dicentric Chromosom...mentioning

confidence: 99%

Extension of lymphocyte viability for radiation biodosimetry: Potential implications for radiological/nuclear mass casualty incidents

Smith

Escalona

Ryan

et al. 2018

J of Cellular Biochemistry

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Dicentric chromosome assay (DCA) is routinely used for estimating the absorbed radiation dose in exposed humans. Optimal lymphocyte viability is crucial for reliable dose estimation and most cytogenetic laboratories prefer the receipt of blood samples within 24 to 36 hours after collection. Delays in the shipment/receipt of samples can occur sometimes under certain unforeseen circumstances: (1) Adverse weather conditions, (2) distant location of blood collection sites, and (3) shipping and handling of a large number of samples after radiological/nuclear mass casualty incident(s). To circumvent some of these limitations, we evaluated the suitability of ex vivo irradiated blood samples stored in the presence of phytohemagglutinin (PHA) for 7 days at ambient temperature (22‐24°C) for radiation biodosimetry. Blood samples stored in the presence of PHA for up to 7 days showed a higher mitotic index than blood samples stored without PHA. To verify the use of stored blood samples for DCA, frequencies of X‐rays induced dicentric chromosomes were analyzed in the blood samples that were cultured either 24 hours after exposure or 7 days later after storage. Our results indicate that storage of ex vivo irradiated blood samples in the presence of PHA at ambient temperature was found optimal for DCA and that the radiation doses estimated by dicentric chromosome frequencies were grossly similar between the fresh and stored blood samples. Our study suggests that reliable and accurate biodosimetry results can be obtained for triage using blood samples stored for up to a week at ambient temperature in the presence of PHA.

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Blood Storage and the Rate of Chromosome Aberrations after in Vitro Exposure to Ionizing Radiations

Cited by 18 publications

References 5 publications

A Novel Ex Vivo Approach Based on Proteomics and Biomarkers to Evaluate the Effects of Chrysene, MEHP, and PBDE-47 on Loggerhead Sea Turtles (Caretta caretta)

A Novel Ex Vivo Approach Based on Proteomics and Biomarkers to Evaluate the Effects of Chrysene, MEHP, and PBDE-47 on Loggerhead Sea Turtles (Caretta caretta)

Dose Response Relationships Beween Chromosome Damage and Absorbed Radiation

Extension of lymphocyte viability for radiation biodosimetry: Potential implications for radiological/nuclear mass casualty incidents

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