Blood oxygen content measured by oxygen tension after release by carbon monoxide

Klingenmaier, C. H.; Behar, Marjam G.; Smith, Teresa

doi:10.1152/jappl.1969.26.5.653

Cited by 16 publications

(7 citation statements)

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“…One oxygen electrode was used for measurements at high Po2, another at low Po2 (i.e., venous blood), to minimize the errors and delays resulting from electrode hysteresis. The high Po2 was also used for analyses of blood 02 content by the method of Klingenmaier, Behar, and Smith (13), in which Po2 is determined after 50-fold anaerobic dilution of blood with CO-saturated water. Hemoglobin and oxygen saturation were measured with an Instrumentation Laboratories Model 182 CO-Oximeter.…”

Section: Subjectsmentioning

confidence: 99%

Pulmonary Artery Pressure and Alveolar Gas Exchange in Man during Acclimatization to 12,470 ft

Kronenberg¹,

Šafář²,

Leej³

et al. 1971

J. Clin. Invest.

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A B S T R A C T Pulmonary hemodynamics and Rotta, Canepa, Hurtado, Velasquez, and Chavez in 1956 (1) and has been confirmed many times since, both in acclimatized natives (2, 3) and in newcomers (4). The relationship of this pulmonary hypertension to the development of high altitude pulmonary edema (HAPE) is uncertain, but pulmonary artery pressure is higher during episodes of HAPE than during control observations in the same individuals (5), and persons who have had such episodes previously have higher pulmonary artery pressures when reexposed to altitude than nonsusceptible individuals under the same conditions (6). Although it is recognized that the peak incidence of HAPE occurs 1-3 days after entry to high altitude (7), it has not been established whether pulmonary arterial pressure rises in a progressive fashion over the same time course. A progressive rise in pulmonary arterial pressure during a 6 wk period at 12,700 ft has been reported in steers, apparently associated with the right heart failure termed "brisket disease" (8), but was not observed in lambs in a matched experiment (9). Therefore, we decided to investigate in ourselves the time course of the pulmonary arterial pressure both at rest and during exercise at 12,470 ft and to examine other aspects of cardiovascular function over the same time span. We also explored the possibility suggested by Haab, Held, Ernst, and Farhi (10) and Reeves, Halpin, Cohn, and Daoud (11) that hypoxia widens the alveolar to arterial 02 difference.

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Section: Subjectsmentioning

confidence: 99%

Pulmonary Artery Pressure and Alveolar Gas Exchange in Man during Acclimatization to 12,470 ft

Kronenberg¹,

Šafář²,

Leej³

et al. 1971

J. Clin. Invest.

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“…The oxygen combined with haemoglobin is released into solution and the resulting change in oxygen tension of the ferricyanide solution (or CO-saline) is related to the oxygen content of the blood. A number of workers have described suitable apparatus and correlation with the Van Slyke technique has been satisfactory (Linden, Ledsome and Norman, 1965;Klingenmaier, Behar and Smith, 1969;Hedden, 1970;Solymar, Rucklidge and Prys-Roberts, 1971). where Hb is the haemoglobin concentration (g dl" 1 ), 5o 2 is the fractional oxygen saturation, F is the oxygen combining factor for haemoglobin (ml g" 1 ), Po 2 is the oxygen tension (kPa) and 5 is the solubility coefficient for oxygen in blood at 37 °C (normally 0.0225 ml dl" 1 kPa" 1 ).…”

Section: Oxygen Contentmentioning

confidence: 99%

What Is New in Blood-Gas Analysis?

Blackburn

1978

British Journal of Anaesthesia

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“…Since then, several papers have dealt with refinements of this technique. Layer et al (10) and Tucker (11) used ferricyanide to release oxygen from hemoglobin, and Awad and Winzler (12) and Klingenmaier et al (13) introduced the use of carbon monoxide to displace oxygen from hemoglobin without lysing the cells. Maio and Neville (14) estimated both oxygen content and oxygen capacity by the polarographic dropping-mercury electrode.…”

Section: The Determination Of Oxygen Capacity and Oxygen Saturationmentioning

confidence: 99%

“…All of these techniques, however, are based on the irreversible transformation of one or various derivatives of hemoglobin. The ferncyanide method (10), for instance, involves the transformation of Hb, Hb02, and possibly some HbCO into Hb+, whereas the carbon monoxide method (13) causes the transformation of Hb and Hb02 into HbCO. Thus, the former method will prevent the determination of methemoglobin on the same sample, whereas the latter method will not be suitable for the determination of the HbCO concentration.…”

Section: The Determination Of Oxygen Capacity and Oxygen Saturationmentioning

confidence: 99%

Simultaneous determination of hemoglobin derivatives, oxygen content, oxygen capacity, and oxygen saturation in 10 microliters of whole blood.

Rossi-Bernardi¹,

Perella²,

Luzzana³

et al. 1977

Clinical Chemistry

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We describe a new method for simultaneous determination of four hemoglobin derivatives (deoxyhemoglobin, Hb; oxyhemoglobin, HbO2; methemoglobin, Hb+; and carbon monoxide hemoglobin, HbCO) and total oxygen content in 10 microliters of whole blood. Percentage HbO2, HbCO, Hb+, total hemoglobin (Hbt), and oxygen capacity can also be obtained from the experimental data by simple calculations. Total analysis time is 1 min. Blood is diluted 100-fold with a buffer contained in a quasi-anaerobic cuvette, where simultaneous measurements of oxygen pressure (by a po2 electrode) and adsorbance (at 497, 565, and 620 nm) are made. The decrease of oxygen pressure, as recorded by the oxygen electrode, is proportional to the amount of deoxyhemoglobin. The concentrations of HbO2, HbCO, and Hb+ can be obtained from absorbance measurements at the specified wavelengths. The new method eliminates the use of short-path optical cells and, due to the low sample volume requirement, makes possible the automated measurement of hemoglobin derivatives and oxygen saturation in arterialized capillary blood.

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Blood oxygen content measured by oxygen tension after release by carbon monoxide

Cited by 16 publications

References 0 publications

Pulmonary Artery Pressure and Alveolar Gas Exchange in Man during Acclimatization to 12,470 ft

Pulmonary Artery Pressure and Alveolar Gas Exchange in Man during Acclimatization to 12,470 ft

What Is New in Blood-Gas Analysis?

Simultaneous determination of hemoglobin derivatives, oxygen content, oxygen capacity, and oxygen saturation in 10 microliters of whole blood.

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