Blood Levels of Neuropeptide 26RFa in Relation to Anxiety and Aggressive Behavior in Humans—An Exploratory Study

Abstract

26RFa, also referred to as QRFP, is a hypothalamic neuropeptide mainly known for its role in the regulation of appetite and glucose metabolism. Its possible relevance to emotional regulation is largely unexplored. To address this, in the present exploratory study, we analyzed the plasma concentrations of 26RFa in humans characterized by different levels of anxiety and aggressive behavior. For this purpose, the study included 13 prison inmates who have committed violent crimes and 19 age-matched healthy men from the general population as controls. Anxiety, depression and aggressive behavior were evaluated in both groups using standard questionnaires. The inmate group was characterized by increased aggression and anxiety compared to the controls. We found that the mean plasma levels of 26RFa did not significantly differ between the inmates and the controls. However, several high outliers were present only in the inmate group. The plasma levels of 26RFa correlated positively with the anxiety scores in all the studied subjects and controls. After removing the high outliers in the inmate group, positive correlations of 26RFa with anxiety and a subscale of hostility in the aggression scale were also recorded in this group. No significant correlations of 26RFa with depression scores or other parameters of aggressive behavior were found. Thus, the present results did not support an involvement of 26RFa in aggressive behavior in humans but pointed to a link between this neuropeptide and anxiety. Nevertheless, considering the exploratory nature of the present study, this conclusion should be verified in a larger cohort, including the clinical degree of anxiety.

Neuropeptide QRFP decreased food intake in the lateral hypothalamus

“…In agreement with these data, another study established anxiolytic effect of GPR103 (QRFP) receptor agonist in mice [41]. Interestingly, a recent human study on aggressive individuals revealed that the plasma levels of QRFP positively correlated with the anxiety scores in all the studied subjects and controls [42]. Nevertheless, in our study QRFP applied into the LHA did not modify general locomotion, nor the anxiety level of tested animals (Figs.…”