Blood group genotyping in multi-transfused patients

Bakanay, Şule Mine; Öztürk, Ayşenur; İleri, Talia; İnce, Elif; Yavasoglu, Suzan; Akar, Nejat; Uysal, Zümrüt; Arslan, Önder

doi:10.1016/j.transci.2013.01.009

Cited by 32 publications

(34 citation statements)

References 34 publications

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“…In this study, the concordance rate between DNA‐based testing and serologic confirmation was 0.9997, similar to a 0.9995 concordance rate observed in a comparison of 356 reagent donor RBCs analyzed by hemagglutination and genotype using the same high‐throughput DNA platform used here . Fifty‐seven patients (11.5%) had at least one serologic discrepancy noted, compared to studies in multiply transfused patients with thalassemia and SCD that reported serologic and DNA‐predicted phenotype discrepancies in 36% to 51% of individuals . The lower rate of discordant results in this study likely reflects the policy of obtaining an extended RBC phenotype by age 1 year before any transfusion and systematically obtaining the RBC phenotype after a hypotonic saline wash if a patient was recently transfused.…”

Section: Discussionsupporting

confidence: 79%

Changing practice: red blood cell typing by molecular methods for patients with sickle cell disease

et al. 2015

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Section: Discussionsupporting

confidence: 79%

Changing practice: red blood cell typing by molecular methods for patients with sickle cell disease

et al. 2015

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“…In transfusion medicine, SNP typing is a powerful adjunct to serologic testing and is the best method for typing transfused patients . Recent study has shown that molecular matching of patients and RBC units reduced alloimmunization risks .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the presence of antibodies is a limitation to haemagglutination which is the traditional method for testing donor and patient blood group antigens and irregular antibodies. To overcome this problem, genotyping tests have been developed and their benefit in patients care has been reported in a number of clinical setting .…”

Section: Introductionmentioning

confidence: 99%

A simple genotyping procedure without DNA extraction to identify rare blood donors

et al. 2015

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The procedure was optimized for simplicity of use in genotyping platform and would allow not only to supply antigen-matched products to recipients but also to find rare phenotypes. This methodology could also be useful for establishing a donor repository for human platelet antigens (HPA)-matched platelets since the same issues are involved for patients with neonatal alloimmune thrombocytopenia or post-transfusion purpura.

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“…Studies comparing serology and genotyping in multitransfused population such as patients with thalassaemia and SCD have shown that genotyping is superior to serology for resolving discrepancies [31][32][33][34][35]. Use of genotyped matched units has been shown to decrease alloimmunization rates [36], increase hemoglobin levels and in vivo RBC survival, and diminish frequency of transfusions [37][38][39].…”

Section: Serology Limitationmentioning

confidence: 99%

Accuracy of Blood Group Typing in the Management and Prevention of Alloimmunization

Sippert¹,

Volkova²,

Rios³

2021

Human Blood Group Systems and Haemoglobinopathies

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Blood transfusion is an effective therapeutic approach for several hematological conditions including sickle cell disease (SCD), thalassaemia, myelodysplastic syndrome (MDS), and autoimmune hemolytic anemia. It is also often indicated for transplantation and for patients receiving medical treatments for cancer. However, transfusion treatment can lead to the red blood cell (RBC) alloimmunization when an incompatible antigen is inadvertently present in the transfused blood. Alloantibodies can cause RBC destruction and many other complications defeating the purpose of the treatment. The risk of development of multiple alloantibodies increases with the frequency of transfusions in transfusion-dependent patients and can be mitigated by transfusing blood type negative for multiple antigens to prevent hemolysis. This chapter discusses the transfusion's risk of RBC alloimmunization as an adverse event; consequences of alloimmunization in patients' care; approaches to prevent and/or mitigate alloimmunization and enhance transfusion efficacy; application of RBC genotyping to supplement serology for preventing alloimmunization. The currently available techniques for RBC genotyping and the importance of reference reagents for determining the genotyping accuracy will also be discussed.Accuracy of Blood Group Typing in the Management and Prevention of Alloimmunization DOI: http://dx.doi.org/10.5772/intechopen.90095 Blood group system WHO IRR [98] CBER RR [89]Rh RHD positive (zygosity not determined)

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Blood group genotyping in multi-transfused patients

Cited by 32 publications

References 34 publications

Changing practice: red blood cell typing by molecular methods for patients with sickle cell disease

Changing practice: red blood cell typing by molecular methods for patients with sickle cell disease

A simple genotyping procedure without DNA extraction to identify rare blood donors

Accuracy of Blood Group Typing in the Management and Prevention of Alloimmunization

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