Blood group and size dependent stability of <i>P. falciparum</i> infected red blood cell aggregates in capillaries

Jötten, Anna Martina; Moll, Kirsten; Wahlgren, Mats; Wixforth, Achim; Westerhausen, Christoph

doi:10.1063/1.5125038

Cited by 16 publications

(34 citation statements)

References 25 publications

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“…Erythrocytes with non-O blood groups have been suggested to be more susceptible to complicated or severe forms of the disease [29]. The blood group A and B antigens have been identified to enhance the rosetting ability of P. falciparum, a phenomenon that contributes to the pathogenesis of severe malaria by causing occlusion of capillaries that supply oxygenated blood to vital organs such as the brain [3,31,32].…”

Section: Discussionmentioning

confidence: 99%

Asymptomatic carriage of Plasmodium falciparum by individuals with variant blood groups and haemoglobin genotypes in southern Ghana

Acquah

Donu

Bredu

et al. 2020

Malar J

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Background: The ABO and the Rhesus blood group systems, as well as various abnormal haemoglobin (Hb) variants (haemoglobinopathies) are known to influence malaria parasite carriage and disease severity in individuals living in malaria endemic areas. This study identified the blood group and Hb variant distribution and Plasmodium falciparum infection status of afebrile individuals living in southern Ghana. Methods: Afebrile participants were recruited from Obom (358) in the Greater Accra Region and Ewim (100) and Simiw (329) in the Central Region of Ghana. Venous blood (1 ml) was collected into EDTA vacutainer tubes. Three 20 μl drops of blood were used for blood group analysis using the tile method. Another 500 μl aliquot was used for the qualitative sickling test using sodium metabisulphite and haemoglobin electrophoresis. Genomic DNA was extracted from 100 μl of whole blood and used in P. falciparum species-specific PCR. Results: The most abundant blood group and abnormal haemoglobin variant in both sites was blood group O + (47.4%) and HbAS (15.8%). A total of 13 (1.7%) of the participants had full haemoglobinopathies (SS, SC and CC), whilst 196 (25.4%) were carriers (AS and AC). Although there was a significantly higher prevalence of sickling positive participants from the Central Region, genotyping identified a similar prevalence of each of the abnormal haemoglobin genes in both sites. Asymptomatic parasite carriage estimated by PCR was 40.9% in the Central Region and 41.8% in the Greater Accra Region. Conclusions: Asymptomatic carriage of P. falciparum parasite in the study population was not associated with any particular blood group variant or haemoglobin genotype.

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Section: Discussionmentioning

confidence: 99%

Asymptomatic carriage of Plasmodium falciparum by individuals with variant blood groups and haemoglobin genotypes in southern Ghana

Acquah

Donu

Bredu

et al. 2020

Malar J

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“…The videos were recorded with the software Photron Fastcam Viewer PFV from Photron at 2000 frames per second, 1280 × 120 pixel resolution, 1/40 000 s exposure resulting in a maximum total of 74 531 frames or 37 s in real time covering an area of 704 × 66 μm². For analysis, we adapted our customized software SURF, introduced earlier [18], [19].…”

Section: Microfluidic Methods Microchannel Design and Measurement Setupmentioning

confidence: 99%

“…Exemplary micrographs are shown in Figure 4A. The cells are traced from frame to frame using the customized MATALB script SURF [19].…”

Section: Elasticity Of Melanoma Cells -Microfluidic Methodsmentioning

confidence: 99%

Dynamic Effective Elasticity of Melanoma Cells Under Shear and Elongational Flow Confirms Estimation From Force Spectroscopy

Jötten¹,

Neidinger²,

Tietze³

et al. 2021

Preprint

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The detection and enrichment of circulating melanoma cells is a challenge as the cells are very heterogeneous in terms of their biomechanical properties and surface markers. In addition, there is a lack of valid and reliable biomarkers that predict progress and therapeutic response. We here analyzed the elasticity of A375 melanoma cells applying force spectroscopy and a microfluidic method. To identify and eventually separate circulating tumor cells, it is crucial to precisely know their physical properties. First, we used standard AFM force spectroscopy, where the elasticity of the cells is calculated from indentation with a pyramidal tip. To extend the limits of measurement with a tip, we then used cantilevers without a tip to press on the cells over a large area. The resulting Young’s moduli are slightly lower and vary less without tip presumably because of the inhomogeneity of the cells. Finally, we implemented our microfluidic method. We measured single cell elasticity by analyzing its deformation in high-speed micrographs while passing a stenosis. Combining the force field and the change in shape provides the basis for a stress strain diagram. The results from microfluidic deformation analysis were in accordance with the results from force spectroscopy. The microfluidic method provides advantages over conventional methods, since it is less invasive and less likely to harm the cell during the measurement, and the whole cell is measured as one entity without contact to a stiff substrate, while force spectroscopy is limited to the contact area of the tip, and in some cases dependent of the cell substrate interaction. Consequently, microfluidic deformation analysis allows to predict the overall elastic behavior of the whole inhomogeneous cell in three-dimensional force fields. This method may contribute to improve the detection of circulation melanoma cells in the clinical practice.

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“…The videos were recorded with the software Photron Fastcam Viewer PFV from Photron at 2000 frames per second, 1280 × 120 pixel resolution, 1/40,000 s exposure resulting in a maximum total of 74 531 frames or 37 s in real time, covering an area of 704 × 66 µm 2 . For analysis, we adapted our customized software SURF, introduced earlier [26,27].…”

Section: Microfluidic Methods Microchannel Design and Measurement Setupmentioning

confidence: 99%

“…Finally, the deformation of A375 cells is analyzed during passage of a microfluidic channel with a diameter of 27 × 50 µm 2 containing a stenosis of 27 × 5 µm 2 , as shown in Figure 4A. We chose these dimensions based on a size analysis of the cells and prior studies with erythrocyte aggregates [27]. At a flow rate of ~75 µL/h, high speed micrographs are recorded at 2000 fps and 1/40,000 s exposure.…”

Section: Elasticity Of Melanoma Cells-microfluidic Methodsmentioning

confidence: 99%

Dynamic Effective Elasticity of Melanoma Cells under Shear and Elongational Flow Confirms Estimation from Force Spectroscopy

et al. 2021

Self Cite

View full text Add to dashboard Cite

The detection and enrichment of circulating melanoma cells is a challenge, as the cells are very heterogeneous in terms of their biomechanical properties and surface markers. In addition, there is a lack of valid and reliable biomarkers predicting progress and therapeutic response. In this study, we analyze the elasticity of A375 melanoma cells by applying force spectroscopy and a microfluidic method. To identify and eventually separate freely circulating tumor cells, it is crucial to know their physical properties precisely. First, we use standard AFM force spectroscopy, where the elasticity of the cells is calculated from indentation with a pyramidal tip. To extend the limits of the measurements with a tip, we then use cantilevers without a tip to apply force over a larger area of the cells. The resulting Young’s moduli are slightly lower and vary less without the tip, presumably because of the spatial inhomogeneity of the cells. Finally, we implement our microfluidic method: we measure single cell elasticity by analyzing their deformation in high-speed micrographs while passing a stenosis. Combining the force field and the change in shape provides the basis for a stress–strain diagram. The results from the microfluidic deformation analysis were well in accordance with the results from force spectroscopy. The microfluidic method, however, provides advantages over conventional methods, as it is less invasive and less likely to harm the cell during the measurement. The whole cell is measured as one entity without having contact to a stiff substrate, while force spectroscopy is limited to the contact area of the tip, and in some cases dependent of the cell substrate interaction. Consequently, microfluidic deformation analysis allows us to predict the overall elastic behavior of the whole, inhomogeneous cell in three-dimensional force fields. This method may contribute to improve the detection of circulating melanoma cells in the clinical practice.

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Blood group and size dependent stability of P. falciparum infected red blood cell aggregates in capillaries

Abstract: Blood group and size dependent stability of P. falciparum infected red blood cell aggregates in capillaries

Cited by 16 publications

References 25 publications

Asymptomatic carriage of Plasmodium falciparum by individuals with variant blood groups and haemoglobin genotypes in southern Ghana

Asymptomatic carriage of Plasmodium falciparum by individuals with variant blood groups and haemoglobin genotypes in southern Ghana

Dynamic Effective Elasticity of Melanoma Cells Under Shear and Elongational Flow Confirms Estimation From Force Spectroscopy

Dynamic Effective Elasticity of Melanoma Cells under Shear and Elongational Flow Confirms Estimation from Force Spectroscopy

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