No studies to date came up with fruitful consequences regarding if or not there is a relationship between blood groups and personality traits rather providing most conflicting, floundering, discrete, equivocal outcomes. Traditionally, Japanese and Korean people support the conception that there are natural inclinations of personality traits in blood groups. Our study aims to explore the rationality of this conception that personality traits and blood groups are interrelated. However, responses of the participants were collected using 50 items big-five factor personality inventory developed by Goldberg: Extraversion, Agreeableness, Neuroticism, Conscientiousness, Intellect (Openness to experience). A two way multivariate analysis (MANOVA) was performed on total 148 participants including 85 males and 65 females using IBM SPSS Statistics version 26. MANOVA results revealed that neither the significant main effect of blood groups [F (15,414) = 1.102, p > 0.05] nor had the significant interaction effect of blood groups and gender [F (15,414) = 1.111, p > 0.05] on the combined dependent variables. But this study found significant main effect of gender on the combined dependent variables [F (5,136) = 4.520, p = 0.001, (1-β) = 0.967, η2 = 0.143].This present study does not support the idea that there is significant relationship between blood groups and personality traits. But the idea that male personality significantly differs from female personality is well-supported by this study.