Blood Group ABO Antigen Expression in Human Embryonic Stem Cells and in Differentiated Hepatocyte- and Cardiomyocyte-Like Cells

Mölne, Johan; Björquist, Petter; Andersson, Kjell; Diswall, Mette; Jeppsson, Anders; Strokan, Valeri; Rydberg, Lennart; Breimer, Michael E.

doi:10.1097/tp.0b013e31818a6805

Cited by 26 publications

(25 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hESC lines were derived and characterized as previously described (11, 12). The hiPSC lines ChiPSC-7, ChiPSC-9, and P11012 were Cellectis in house human induced pluripotent stem cells derived by the retroviral transduction technique, whereas the derivation of ChiPSC-4 was done as described (13).…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemical analyses were performed as described (12). The hiPSC lines (ChiPSC-4, ChiPSC-7, and ChiPSC-9), and four of the hESC lines (SA002, SA121, SA181, and AS038) were cultured under feeder-free conditions, whereas the remaining three hESC lines (SA001, SA348, and SA461) were cultured on mitomycin-C-inactivated mouse embryoid fibroblast feeder layers.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells

Barone

Säljö

Benktander³

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Background: Carbohydrate epitopes are often used as markers for characterization of human pluripotent stem cells (hPSC).Results: Sialyl-lactotetra is highly expressed on the cell surface of hPSC lines, and the expression decreased upon early differentiation.Conclusion: Sialyl-lactotetra is a novel marker of undifferentiated human stem cells.Significance: These findings might impact future applications in regenerative medicine.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells

Barone

Säljö

Benktander³

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…(22,23) It is also reported that BG-H2 is expressed in early stage hematopoietic cells and embryonic stem cells. (24) However, the expression of BG-H2 in CSCs, particularly in colon cancer CSCs, is unknown. In this study we found that BG-H2 antigen was overexpressed on the surface of colon CSCs (CD133þ) (Fig.…”

Section: Resultsmentioning

confidence: 99%

MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion

Wang

Gildersleeve

et al. 2010

Hybridoma

View full text Add to dashboard Cite

The functions of the precursor H antigen for ABO blood group antigens are still not fully understood, particularly in cancer cells. In this study, we used hybridoma technology and NSY human colon cancer cells as an immunogen to generate a monoclonal antibody designated as MAb L9E10. The binding antigen of MAb L9E10 was identified as blood group (BG) H2 antigen using carbohydrate array and erythrocyte agglutination assays. In immunofluorescence study, we found that BG-H2 was expressed on the surfaces of both colon cancer stem cells and their differentiated progeny. In a functional study, we observed that MAb L9E10 inhibited tumor cell migration and invasion at a concentration of 10 mg/mL in vitro. This result suggests that MAb L9E10 could be used to study cancer biology, particularly cancer stem cell biology. In addition, it is potentially useful for studying gastric diseases caused by Helicobacter pylori bacteria, with attachment to human gastric epithelial cells mediated by blood group antigens Lewis b and H2. Finally, MAb L9E10 is an ideal biological reagent for identifying Bombay blood type in which erythrocytes have no BG-H2 antigen expression.

show abstract

“…All three are expected to be found in cell populations used to create cardiac grafts. A or B blood group antigens are expressed on ESC and ESC-derived cells [53]. These antigens are critical for safe blood transfusions.…”

Section: Antigens Classes For Stem Cell-derived Cardiomyocytesmentioning

confidence: 99%

Immunological Barriers to Stem-Cell Based Cardiac Repair

Karabekian

Posnack

Sarvazyan

2010

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Repair of damaged myocardium with pluripotent stem cell derived cardiomyocytes is becoming increasingly more feasible. Developments in stem cell research emphasize the need to address the foreseeable problem of immune rejection following transplantation. Pluripotent stem cell (PSC) derived cardiomyocytes have unique immune characteristics, some of which are not advantageous for transplantation. Here we review the possible mechanisms of PSC-derived cardiomyocytes rejection, summarize the current knowledge pertaining to immunogenicity of such cells and describe the existing controversies. Myocardial graft rejection can be reduced by modifying PSCs prior to their differentiation into cardiomyocytes. Overall, this approach facilitates the development of universal donor stem cells suitable for the regeneration of many different tissue types.

show abstract

Blood Group ABO Antigen Expression in Human Embryonic Stem Cells and in Differentiated Hepatocyte- and Cardiomyocyte-Like Cells

Cited by 26 publications

References 26 publications

Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells

Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells

MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion

Immunological Barriers to Stem-Cell Based Cardiac Repair

Contact Info

Product

Resources

About