Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells

Barone, Angela; Säljö, Karin; Benktander, John; Blomqvist, Maria; Månsson, Jan‐Eric; Johansson, Bengt R.; Mölne, Johan; Aspegren, Anders; Björquist, Petter; Breimer, Michael E.; Teneberg, Susann

doi:10.1074/jbc.m114.568832

Cited by 27 publications

(37 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In humans SSEA-4 is a marker for embryonic and adult stem cells [ 34 – 36 ]. Upon differentiation of human embryonic stem cells, the expression of SSEA-4 is rapidly diminished, and has almost completely disappeared after 14 days [ 37 , 38 ], i.e., SSEA-4 downregulation accompanies the loss of pluripotency.…”

Section: Discussionmentioning

confidence: 99%

Characterization of moose intestinal glycosphingolipids

et al. 2015

Self Cite

View full text Add to dashboard Cite

As a part of a systematic investigation of the species-specific expression of glycosphingolipids, acid and non-acid glycosphingolipids were isolated from three small intestines and one large intestine of the moose (Alces alces). The glycosphingolipids were characterized by binding of monoclonal antibodies, lectins and bacteria in chromatogram binding assays, and by mass spectrometry. The non-acid fractions were complex mixtures, and all had glycosphingolipids belonging to the lacto- and neolactoseries (lactotriaosylceramide, lactotetraosylceramide, neolactotetraosylceramide, Galα3-Lex hexaosylceramide, and lacto-neolactohexaosylceramide), globo-series (globotriaosylceramide and globotetraosylceramide), and isogloboseries (isoglobotriaosylceramide). Penta- and heptaglycosylceramides with terminal Galili determinants were also characterized. Furthermore, glycosphingolipids with terminal blood group O determinants (H triaosylceramide, H type 2 pentaosylceramide, H type 1 penta- and heptaosylceramide) were characterized in two of the moose small intestines, and in the one large intestine, while the third small intestine had glycosphingolipids with terminal blood group A determinants (A tetraosylceramide, A type 1 hexa- and octaosylceramide, A dodecaosylceramide). The acid glycosphingolipid fractions of moose small and large intestine contained sulfatide, and the gangliosides GM3, GD3, GD1a, GD1b, and also NeuGc and NeuAc variants of the Sda ganglioside and the sialyl-globopenta/SSEA-4 ganglioside. In humans, the NeuAc-globopenta/SSEA-4 ganglioside is a marker of embryonic and adult stem cells, and is also expressed in several human cancers. This is the first time sialyl-globopentaosylceramide/SSEA-4 has been characterized in a fully differentiated normal tissue, and also the first time NeuGc-globopentaosylceramide has been characterized.Electronic supplementary materialThe online version of this article (doi:10.1007/s10719-015-9604-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of moose intestinal glycosphingolipids

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…The CID spectrum of m/z 824.5 reveals a mass loss of 80, which is characteristic for a sulfate group, plus the same compounds that can be detected in the case of sterlet at m/z 722.5, i.e., both compounds have similar structures. The fragmentation of m/z 778 resulted in losses of m/z 242 and 256, which are characteristic of a sulfated hexose moiety [42,43]. It is known that sulfated glycolipids can be basically detected in the positive and in the negative ion mode [44].…”

Section: Tandem Ms Experiments To Elucidate the Assignments Of Unknowmentioning

confidence: 99%

Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization

et al. 2020

View full text Add to dashboard Cite

The lipid composition of sperm membranes is crucial for fertilization and differs among species. As the evolution of internal fertilization modes in fishes is not understood, a comparative study of the sperm lipid composition in freshwater representatives of externally and internally fertilizing fishes is needed for a better understanding of taxa-specific relationships between the lipid composition of the sperm membrane and the sperm physiology. The lipidomes of spermatozoa from stingray, a representative of cartilaginous fishes possessing internal fertilization, and sterlet, a representative of chondrostean fishes with external fertilization, have been studied by means of nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray MS, gas chromatography-(GC) MS, and thin-layer chromatography (TLC). NMR experiments revealed higher cholesterol content and the presence of phosphatidylserine in stingray compared to sterlet sperm. Unknown MS signals could be assigned to different glycosphingolipids in sterlet (neutral glycosphingolipid Gal-Cer(d18:1/16:0)) and stingray (acidic glycosphingolipid sulpho-Gal-Cer(d18:1/16:0)). Free fatty acids in sterlet sperm indicate internal energy storage. GC-MS experiments indicated a significant amount of adrenic acid, but only a low amount of docosahexaenoic acid in stingray sperm. In a nutshell, this study provides novel data on sperm lipid composition for freshwater stingray and sterlet possessing different modes of fertilization.

show abstract

“…The target of the TRA-1-60 antibody, the nonsialylated version of the glycan, is an excellent marker for pluripotent stem cells. 30 , 31 , 32 Previous research found sialyl-LacNAc type 1 on glycolipids in malignant glioma 33 and embryonal carcinoma. 34 Pancreatic cancer cells frequently activate developmental pathways, 35 , 36 , 37 potentially leading to the expression of the sialyl-LacNAc type 1 epitope.…”

Section: Discussionmentioning

confidence: 97%

Glycans Related to the CA19-9 Antigen Are Increased in Distinct Subsets of Pancreatic Cancers and Improve Diagnostic Accuracy Over CA19-9

Tang

Partyka

Hsueh

et al. 2016

Cellular and Molecular Gastroenterology and Hepatology

View full text Add to dashboard Cite

Background and Aims The CA19-9 antigen is the current best biomarker for pancreatic cancer, but it is not elevated in about 25% of pancreatic cancer patients at a cutoff that gives a 25% false-positive rate. We hypothesized that antigens related to the CA19-9 antigen, which is a glycan called sialyl-Lewis A (sLeA), are elevated in distinct subsets of pancreatic cancers. Methods We profiled the levels of multiple glycans and mucin glycoforms in plasma from 200 subjects with either pancreatic cancer or benign pancreatic disease, and we validated selected findings in additional cohorts of 116 and 100 subjects, the latter run blinded and including cancers that exclusively were early-stage. Results We found significant elevations in two glycans: an isomer of sLeA called sialyl-Lewis X, present both in sulfated and non-sulfated forms; and the sialylated form of a marker for pluripotent stem cells, type 1 N-acetyl-lactosamine. The glycans performed as well as sLeA as individual markers and were elevated in distinct groups of patients, resulting in a 3-marker panel that significantly improved upon any individual biomarker. The panel gave 85% sensitivity and 90% specificity in the combined discovery and validation cohorts, relative to 54% sensitivity and 86% specificity for sLeA; and it gave 80% sensitivity and 84% specificity in the independent test cohort, as opposed to 66% sensitivity and 72% specificity for sLeA. Conclusions Glycans related to sLeA are elevated in distinct subsets of pancreatic cancers and yield improved diagnostic accuracy over CA19-9.

show abstract

Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells

Cited by 27 publications

References 40 publications

Characterization of moose intestinal glycosphingolipids

Characterization of moose intestinal glycosphingolipids

Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization

Glycans Related to the CA19-9 Antigen Are Increased in Distinct Subsets of Pancreatic Cancers and Improve Diagnostic Accuracy Over CA19-9

Contact Info

Product

Resources

About