Blood-forming system in rats after whole-body microwave exposure; reference to the lymphocytes

Trošić, Ivančica; Bušljeta, Ivana; Pavičić, Ivan

doi:10.1016/j.toxlet.2004.07.011

Cited by 22 publications

(7 citation statements)

References 28 publications

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“…In a report by Ragan et al (47) of hematological and immunological effects of microwaves in mice, bone marrow cellularity was significantly reduced in one of six groups exposed at 10 mW/cm2, in contrast with an increase in bone marrow cellularity in two studies exposed at 5 mW/cm2. Trosi et al (48) reported that the number of lymphoblasts in irradiated animals was lower throughout the experiment compared with the matched sham-exposed rats, but a significant decrease was obtained at 15 and 30 days into the experiment. In the same study, the absolute number of lymphocytes in bone marrow did not significantly differ between animals in the treated and untreated groups.…”

Section: Discussionmentioning

confidence: 95%

In Vivo Radioprotective Effects of Nigella sativa L Oil and Reduced Glutathione Against Irradiation-Induced Oxidative Injury and Number of Peripheral Blood Lymphocytes in Rats

Cemek¹,

Enginar²,

Karaca³

et al. 2006

Photochem Photobiol

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Radiotherapy is one of the most common therapies for treating human cancers. Several studies have indicated that irradiation induces reactive oxygen species (ROS), which play an important role in radiation damage of the cell. It has been shown that Nigella sativa L. (NS) and reduced glutathione (GSH) have both an antiperoxidative effect on different tissues and a scavenger effect on ROS. The purpose of this study was to determine the antioxidant and radio-protective roles of NS and GSH against irradiation-induced oxidative injury in an experimental model. The NS group was administrated NS (1 mL/kg body weight), the GSH group was injected GSH (150 mg/kg body weight) and the control group was given physiologic saline solution (1 mL/kg body weight) for 30 consecutive days before exposure to a single dose of 6 Gy of radiation. Animals were sacrificed after irradiation. Malondialdehyde, nitrate, nitrite (oxidative stress markers) and ascorbic acid, retinol, beta-carotene, GSH and ceruloplasmin (nonenzymatic antioxidant markers) levels and peripheral blood lymphocytes were measured in all groups. There were statistically significant differences between the groups for all parameters (P < 0.05). Whole-body irradiation caused a significant increase in blood malondialdehyde, nitrate and nitrite levels. The blood oxidative stress marker levels in irradiated rats that were pretreated with NS and GSH were significantly decreased; however, non-enzymatic antioxidant levels were significantly increased. Also, our results suggest that NS and GSH administration prior to irradiation prevent the number of alpha-naphthyl acetate esterase peripheral blood T lymphocytes from declining. These results clearly show that NS and GSH treatment significantly antagonize the effects of radiation. Therefore, NS and GSH may be a beneficial agent in protection against ionizing radiation-related tissue injury.

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Section: Discussionmentioning

confidence: 95%

In Vivo Radioprotective Effects of Nigella sativa L Oil and Reduced Glutathione Against Irradiation-Induced Oxidative Injury and Number of Peripheral Blood Lymphocytes in Rats

Cemek¹,

Enginar²,

Karaca³

et al. 2006

Photochem Photobiol

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“…The decrease in GSH activity correlates with the increase in lipid per oxidation. This may account for the increased levels of oxidized lipids in the serum lipoproteins of irradiated mice following consumption of a diet rich in oxidized lipids 32 , since the intestinal/hepatic GSH detoxifies dietary lipids before they enter the circulation 33,34 .…”

Section: Discussionmentioning

confidence: 99%

Untitled

2017

IJPSR

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“…Results present in the literature, even when using a variety of animal models and experimental conditions, were unable to solve the issue. In rats, a decrease in the number of lymphoblasts [Trosic et al, ] and an increase of micronuclei in polychromatic erythrocytes [Demsia et al, ] were reported after exposure to RF. Other authors described an increase, a decrease or no effects on BMC numbers after RF‐field exposure, showing no data on functional parameters [Galvin et al, ; Ragan et al, ; Trosic et al, ].…”

Section: Discussionmentioning

confidence: 99%

“…Yet, few studies have assessed the in vivo effects of RF field exposure on BM‐HPC without reaching conclusive results. Indeed, increased, decreased or no change in bone marrow cell (BMC) numbers were reported by different groups but no functional assay was ever been described [Galvin et al, ; Ragan et al, ; Trosic et al, ]. As BM is a site of active lymphocyte/monocyte/erythrocyte cell production, it becomes crucial to assess whether BM‐HPCs from RF‐field‐exposed animals have the same ability of generating mature cells as BM precursor cells isolated from unexposed animals.…”

Section: Introductionmentioning

confidence: 99%

Effects of GSM‐modulated 900 MHz radiofrequency electromagnetic fields on the hematopoietic potential of mouse bone marrow cells

Rosado

Nasta

Prisco

et al. 2014

Bioelectromagnetics

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Studies describing the influence of radiofrequency electromagnetic fields on bone marrow cells (BMC) often lack functional data. We examined the effects of in vivo exposure to a Global System for Mobile Communications (GSM) modulated 900 MHz RF fields on BMC using two transplantation models. X-irradiated syngeneic mice were injected with BMC from either RF-field-exposed, sham-exposed or cage control mice. Twelve weeks after transplantation, no differences in thymocyte number, frequency of subpopulations and cell proliferation were found in mice receiving BMC from either group. Also, in the spleen cell number, percentages of B/T cells, B/T-cell proliferation, and interferon γ (IFN-γ) production were similar in all groups. In parallel, a mixture of BMC from congenic sham- and RF-exposed mice were co-transplanted into lymphopenic Rag2 deficient mice. BMC from RF-exposed and sham-exposed mice displayed no advantage or disadvantage when competing for the replenishment of lymphatic organs with mature lymphocytes in Rag2 deficient mice. This model revealed that BMC from sham-exposed and RF-exposed mice were less efficient than BMC from cage control mice in repopulating the thymus, an effect likely due to restraint stress. In conclusion, our results showed no effects of in vivo exposure to GSM-modulated RF-fields on the ability of bone marrow (BM) precursors to long-term reconstitute peripheral T and B cell compartments.

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Blood-forming system in rats after whole-body microwave exposure; reference to the lymphocytes

Cited by 22 publications

References 28 publications

In Vivo Radioprotective Effects of Nigella sativa L Oil and Reduced Glutathione Against Irradiation-Induced Oxidative Injury and Number of Peripheral Blood Lymphocytes in Rats

In Vivo Radioprotective Effects of Nigella sativa L Oil and Reduced Glutathione Against Irradiation-Induced Oxidative Injury and Number of Peripheral Blood Lymphocytes in Rats

Untitled

Effects of GSM‐modulated 900 MHz radiofrequency electromagnetic fields on the hematopoietic potential of mouse bone marrow cells

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