Blood Flow Visualization and Measurements in Microfluidic Devices Fabricated by a Micromilling Technique

Singhal, Jaron; Pinho, Diana; Lopes, Raquel; Sousa, P.C.; Garcia, Valdemar; Schütte, Helmut; Lima, Rui; Gaßmann, Stefan

doi:10.2174/1876402908666160106000332

Cited by 10 publications

(6 citation statements)

References 25 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this material also has certain limitations that encourage researchers to seek alternative materials, one of them being the possibility of absorption of small hydrophobic species [23,64]. Two alternative materials are poly(-methylmethacrylate) (PMMA) [66][67][68][69] and cyclic olefin copolymer (COC) [70]. Zahorodny-Burke et al [53] studied the nature of oxygen transport within microfluidic cell culture devices considering PDMS, COC, and PMMA through a two-dimensional convection-diffusion mass transfer model.…”

Section: Fluid Flow and Mass Transfermentioning

confidence: 99%

Computational Simulations in Advanced Microfluidic Devices: A Review

et al. 2021

View full text Add to dashboard Cite

Numerical simulations have revolutionized research in several engineering areas by contributing to the understanding and improvement of several processes, being biomedical engineering one of them. Due to their potential, computational tools have gained visibility and have been increasingly used by several research groups as a supporting tool for the development of preclinical platforms as they allow studying, in a more detailed and faster way, phenomena that are difficult to study experimentally due to the complexity of biological processes present in these models—namely, heat transfer, shear stresses, diffusion processes, velocity fields, etc. There are several contributions already in the literature, and significant advances have been made in this field of research. This review provides the most recent progress in numerical studies on advanced microfluidic devices, such as organ-on-a-chip (OoC) devices, and how these studies can be helpful in enhancing our insight into the physical processes involved and in developing more effective OoC platforms. In general, it has been noticed that in some cases, the numerical studies performed have limitations that need to be improved, and in the majority of the studies, it is extremely difficult to replicate the data due to the lack of detail around the simulations carried out.

show abstract

Section: Fluid Flow and Mass Transfermentioning

confidence: 99%

Computational Simulations in Advanced Microfluidic Devices: A Review

et al. 2021

View full text Add to dashboard Cite

show abstract

“…( b ) cell-free layer as an advantage for cell and plasma separation and plasma skimming effect, WBCs margination. Adapted from [86,95,96]. ( c ) the Bifurcation law manipulated to remove cell-free plasma from blood and to mimic the microvasculature networks.…”

Section: Figurementioning

confidence: 99%

Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications

et al. 2019

Self Cite

View full text Add to dashboard Cite

Since the first microfluidic device was developed more than three decades ago, microfluidics is seen as a technology that exhibits unique features to provide a significant change in the way that modern biology is performed. Blood and blood cells are recognized as important biomarkers of many diseases. Taken advantage of microfluidics assets, changes on blood cell physicochemical properties can be used for fast and accurate clinical diagnosis. In this review, an overview of the microfabrication techniques is given, especially for biomedical applications, as well as a synopsis of some design considerations regarding microfluidic devices. The blood cells separation and sorting techniques were also reviewed, highlighting the main achievements and breakthroughs in the last decades.

show abstract

“…Individually applied in separate setups, 3D printing, CNC micro-milling and laser engraving have been tested in low-cost basic or in more expensive, technically advanced devices in fabricating microfluidic devices and shown to be viable alternatives to conventional photolithography [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. CO 2 laser-based fabrication of microfluidic substrates has, for example, been performed with common materials such as glass and poly methylmethacrylate (PMMA) sheets and the influence of the laser settings and the length and strength of surface treatments on the production of optimal microchannel profiles and on surface smoothness was demonstrated [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

A Low-Cost 3-in-1 3D Printer as a Tool for the Fabrication of Flow-Through Channels of Microfluidic Systems

Thaweskulchai

Schulte

2021

Micromachines

View full text Add to dashboard Cite

Recently published studies have shown that microfluidic devices fabricated by in-house three-dimensional (3D) printing, computer numerical control (CNC) milling and laser engraving have a good quality of performance. The 3-in-1 3D printers, desktop machines that integrate the three primary functions in a single user-friendly set-up are now available for computer-controlled adaptable surface processing, for less than USD 1000. Here, we demonstrate that 3-in-1 3D printer-based micromachining is an effective strategy for creating microfluidic devices and an easier and more economical alternative to, for instance, conventional photolithography. Our aim was to produce plastic microfluidic chips with engraved microchannel structures or micro-structured plastic molds for casting polydimethylsiloxane (PDMS) chips with microchannel imprints. The reproducability and accuracy of fabrication of microfluidic chips with straight, crossed line and Y-shaped microchannel designs were assessed and their microfluidic performance checked by liquid stream tests. All three fabrication methods of the 3-in-1 3D printer produced functional microchannel devices with adequate solution flow. Accordingly, 3-in-1 3D printers are recommended as cheap, accessible and user-friendly tools that can be operated with minimal training and little starting knowledge to successfully fabricate basic microfluidic devices that are suitable for educational work or rapid prototyping.

show abstract

Blood Flow Visualization and Measurements in Microfluidic Devices Fabricated by a Micromilling Technique

Cited by 10 publications

References 25 publications

Computational Simulations in Advanced Microfluidic Devices: A Review

Computational Simulations in Advanced Microfluidic Devices: A Review

Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications

A Low-Cost 3-in-1 3D Printer as a Tool for the Fabrication of Flow-Through Channels of Microfluidic Systems

Contact Info

Product

Resources

About