Blood culture bottles are superior to conventional media for vitreous culture

Thariya, Patsuda; Yospaiboon, Yosanan; Sinawat, Suthasinee; Sanguansak, Thuss; Bhoomibunchoo, Chavakij; Laovirojjanakul, Wipada

doi:10.1111/ceo.12707

Cited by 13 publications

(15 citation statements)

References 18 publications

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“…In the present study, both conventional culture media and blood culture bottles were used for culture of the vitreous fluid specimens, because this laboratory’s previous studies revealed that vitreous culture in blood culture bottles yielded more positive results than conventional culture media, and a combination of both improved the possibility of a positive culture. 8 , 9 The culture yield in this study was only 41.25%. This may be attributed to many factors.…”

Section: Discussionmentioning

confidence: 54%

“… 4 , 6 , 21 This study, however, agrees with previous studies in Thailand. 5 , 8 , 9 This may be attributed to a higher incidence of ocular trauma, especially with vegetative material, in the rural areas. Post-operative endophthalmitis was the next most common and similar to other previous studies, where most of the patients developed infection after cataract surgery.…”

Section: Discussionmentioning

confidence: 99%

“…This finding is also similar to other previous studies. 5 , 8 , 9 The three most common Gram-negative bacteria were Pseudomonas aeruginosa , Klebsiella spp., and Enterobacter spp. This is also in agreement with previous reports.…”

Section: Discussionmentioning

confidence: 99%

“…This is also in agreement with previous reports. 5 , 8 , 9 There were eight eyes with fungal endophthalmitis. Six of them were Aspergillus spp.…”

Section: Discussionmentioning

confidence: 99%

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Factors affecting visual outcomes after treatment of infectious endophthalmitis in northeastern Thailand

Yospaiboon

Intarapanich

Laovirojjanakul

et al. 2018

OPTH

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ObjectiveTo determine factors affecting visual outcomes after treatment of infectious endophthalmitis during 2012–2016 at a large referral eye center in northeastern Thailand.Patients and methodsMedical charts of patients with a diagnosis of infectious endophthalmitis including demographic data, types of endophthalmitis, causative organisms, methods of treatment, anatomical, and functional outcomes were retrospectively reviewed. Factors associated with improved visual outcomes were analyzed.ResultsFour hundred and eleven patients (417 eyes) were recruited for the study. The three most common types were post-traumatic (44.53%), post-operative (31.87%), and endogenous endophthalmitis (17.52%). Vitreous cultures revealed causative organisms in 41.25%. Most common Gram-positive organisms were coagulase-negative Staphylococcus 30% (36/120), Bacillus spp. 20% (24/120), and Streptococcus pneumoniae 18.33% (20/120). Most common Gram-negatives were Pseudomonas spp. 24.32% (9/37), Klebsiella spp. 24.32% (9/37), and Enterobacter spp. 16.21% (6/37). Methods of treatment were medical treatment (18.71%) and surgical treatment (81.29%), including pars plana vitrectomy with or without silicone oil tamponade (62.59%) and destructive surgery (18.71%). After treatment, visual improvement was noted in 44.6%, stable vision in 18.47%, and worse vision in 36.93%. Factors associated with improved visual outcomes were post-operative endophthalmitis (P<0.001), coagulase-negative Staphylococcus (P=0.003), and initial visual acuity before treatment of hand motion or better (P=0.017).ConclusionMost infectious endophthalmitis patients were post-traumatic, post-operative, and endogenous. The most common method of treatment was pars plana vitrectomy. Treatment could improve visual outcomes and at least stabilize the vision in 63.07%. Factors associated with improved visual outcomes were types of endophthalmitis, causative organisms, and initial visual acuity before treatment.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…This is also in agreement with previous reports. 5 , 8 , 9 There were eight eyes with fungal endophthalmitis. Six of them were Aspergillus spp.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Factors affecting visual outcomes after treatment of infectious endophthalmitis in northeastern Thailand

Yospaiboon

Intarapanich

Laovirojjanakul

et al. 2018

OPTH

Self Cite

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“…Conventional culture methods (CM) use solid or broth media including thioglycolate. However, rates of identification increase when blood culture bottles (BCBs) are used (3,6–9).…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis

Tanaka¹,

Ferreira²,

Oliveira³

et al. 2019

Clinics

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OBJECTIVE: To report our experience using conventional culture methods (CM) and pediatric blood culture bottles (PBCBs) for vitreous sample culture of acute postoperative endophthalmitis. METHODS: A retrospective study was conducted at the Department of Ophthalmology, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, BR, from January 2010 to December 2015, and it included 54 patients with clinically suspected acute postoperative endophthalmitis. Vitreous samples were obtained by vitreous tap or vitrectomy. Samples from January 2010 to December 2011 were cultivated in CM, whereas samples from January 2012 to December 2015 were inoculated in PBCBs. The measured outcome was the yield of positive cultures. RESULTS: Twenty cases were included in the CM group, and 34 cases were included in the PBCB group. The yield of positive cultures in PBCBs (64.7%) was significantly higher than that in conventional CM (35%, p =0.034). Staphylococcus epidermidis and Streptococcus viridans were the two most commonly found agents. CONCLUSION: PBCBs can be used successfully in clinically suspected endophthalmitis. The method showed a higher yield of positive cultures than the conventional method. This technique appears to have several advantages over the traditional method: it saves time, as only one medium needs to be inoculated; transportation to a laboratory is easier than in the traditional method, and there is no need to maintain a supply of fresh agar media. The use of PBCBs may be recommended as the primary method for microbiological diagnosis and is especially suitable for office settings and remote clinics.

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Diagnostic performance of blood culture bottles for vitreous culture compared to conventional microbiological cultures in patients with suspected endophthalmitis

Kehrmann

Chapot

Buer

et al. 2018

Eur J Clin Microbiol Infect Dis

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The purpose of this investigation was to evaluate the performance of blood culture bottles in comparison to conventional microbiological culture techniques in detecting causative microorganisms of endophthalmitis and to determine their anti-infective susceptibility profiles. All consecutive cases with clinically suspected endophthalmitis in a university-based ophthalmology department between January 2009 and December 2016 were analysed in this retrospective comparative case series. Samples from 247 patients with suspected endophthalmitis underwent microbiological diagnostic work-up. All three culture methods were performed from 140 vitreous specimens. Vitreous fluid specimens were inoculated in blood culture bottles, aerobic and anaerobic broth solutions, and on solid media. Anti-infective susceptibility profiles were evaluated by semi-automated methods and/or gradient diffusion methods. Microorganisms were grown in 82 of 140 specimens for which all methods were performed (59%). Microorganisms were more frequently grown from blood culture bottles (55%) compared to broth solution (45%, p = 0.007) and solid media (33%, p < 0.0001). Considerable differences in the performance among culture media were detected for fungal pathogens. All grown fungi were detected by blood culture bottles (11 of 11, 100%). Broth solution recovered 64% and solid media 46% of grown fungi. No Gram-positive bacterium was resistant to vancomycin and all Gram-negative pathogens except for one isolate were susceptible to third-generation cephalosporins. In suspected endophthalmitis patients, blood culture bottles have a higher overall pathogen detection rate from vitreous fluid compared to conventional microbiological media, especially for fungi. The initial intravitreal antibiotic therapy with vancomycin plus third-generation cephalosporins appears to be an appropriate treatment approach for bacterial endophthalmitis.

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Blood culture bottles are superior to conventional media for vitreous culture

Abstract: Background: To compare blood culture bottles and conventional media for the vitreous culture in patients with clinically suspected infectious endophthalmitis.

Cited by 13 publications

References 18 publications

Factors affecting visual outcomes after treatment of infectious endophthalmitis in northeastern Thailand

Factors affecting visual outcomes after treatment of infectious endophthalmitis in northeastern Thailand

Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis

Diagnostic performance of blood culture bottles for vitreous culture compared to conventional microbiological cultures in patients with suspected endophthalmitis

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