Blood Compatibility of Cetyl Alcohol/Polysorbate-Based Nanoparticles

Koziara, Joanna M.; Oh, Jennifer J.; Akers, Wendell S.; Ferraris, Suellen P.; Mumper, Russell J.

doi:10.1007/s11095-005-7547-7

Cited by 118 publications

(85 citation statements)

References 28 publications

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“…[60][61][62][63] To assess the in vivo utility of a nanoformulation as a carrier for curcumin, the hemolytic potential in human blood needs to be tested. 64,65 Therefore, we evaluated a direct nanoparticle-erythrocyte membrane interaction in which the extent of disruption of the erythrocyte membrane was a direct measure of nanoparticle toxicity (Figure 4).…”

Section: Hemolysismentioning

confidence: 99%

Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes

Yallapu¹,

Ebeling²,

Chauhan³

et al. 2011

IJN

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Background Recent studies report curcumin nanoformulation(s) based on polylactic- co -glycolic acid (PLGA), β-cyclodextrin, cellulose, nanogel, and dendrimers to have anticancer potential. However, no comparative data are currently available for the interaction of curcumin nanoformulations with blood proteins and erythrocytes. The objective of this study was to examine the interaction of curcumin nanoformulations with cancer cells, serum proteins, and human red blood cells, and to assess their potential application for in vivo preclinical and clinical studies. Methods The cellular uptake of curcumin nanoformulations was assessed by measuring curcumin levels in cancer cells using ultraviolet-visible spectrophotometry. Protein interaction studies were conducted using particle size analysis, zeta potential, and Western blot techniques. Curcumin nanoformulations were incubated with human red blood cells to evaluate their acute toxicity and hemocompatibility. Results Cellular uptake of curcumin nanoformulations by cancer cells demonstrated preferential uptake versus free curcumin. Particle sizes and zeta potentials of curucumin nanoformulations were varied after human serum albumin adsorption. A remarkable capacity of the dendrimer curcumin nanoformulation to bind to plasma protein was observed, while the other formulations showed minimal binding capacity. Dendrimer curcumin nanoformulations also showed higher toxicity to red blood cells compared with the other curcumin nanoformulations. Conclusion PLGA and nanogel curcumin nanoformulations appear to be very compatible with erythrocytes and have low serum protein binding characteristics, which suggests that they may be suitable for application in the treatment of malignancy. These findings advance our understanding of the characteristics of curcumin nanoformulations, a necessary component in harnessing and implementing improved in vivo effects of curcumin.

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Section: Hemolysismentioning

confidence: 99%

Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes

Yallapu¹,

Ebeling²,

Chauhan³

et al. 2011

IJN

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“…The hemolysis assay is usually performed at body temperature (37°C) (Koziara et al 2005) or room temperature (20-25°C) (Hedberg et al 2010;Slowing et al 2009). …”

Section: Influence Of Temperaturementioning

confidence: 99%

Comparative effects of macro-sized aluminum oxide and aluminum oxide nanoparticles on erythrocyte hemolysis: influence of cell source, temperature, and size

et al. 2015

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Al 2 O 3 is the most abundantly produced nanomaterial and has been used in diverse fields, including the medical, military and industrial sectors. As there are concerns about the health effects of nanoparticles, it is important to understand how they interact with cells, and specifically with red blood cells. The hemolysis induced by three commercial nano-sized aluminum oxide particles (nanopowder 13 nm, nanopowder <50 nm and nanowire 2-6 nm × 200-400 nm) was compared to aluminum oxide and has been studied on erythrocytes from humans, rats and rabbits, in order to elucidate the mechanism of action and the influence of size and shape on hemolytic behavior. The concentrations inducing 50% hemolysis (HC 50 ) were calculated for each compound studied.The most hemolytic aluminum oxide particles were of nanopowder 13, followed by nanowire and nanopowder 50. The addition of albumin to PBS induced a protective effect on hemolysis in all the nano-forms of Al 2 O 3 , but not on Al 2 O 3. The drop in HC 50 correlated to a decrease in nanomaterial size, which was induced by a reduction of aggregation Aluminum oxide nanoparticles are less hemolytic than other oxide nanoparticles, and behave differently depending on the size and shape of the nanoparticles. The hemolytic behavior of aluminum oxide nanoparticles differs from that of aluminum oxide.

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“…In case of ACS patients, if nanoparticles activate platelets then they may induce life threatening alarm. Till now there are a number of papers (Geys et al, 2008;Deb et al, 2007Deb et al, ,2011Wiwanitkit et al, 2009;Radomski et al, 2005;Shrivastava et al, 2009;Koziara et al, 2005;Mayer et al, 2009;Li et al, 2009;Ramtoola et al, 2010;McGuinnes et al, 2010;Nemmar et al, 2003;Gulati et al, 2010;Cejas et al, 2007;Wilson et al, 2010;Rückerl et al, 2007) about the effect of nanoparticles on platelets (Table. 2.) where most of the citations show that nanoparticles can induce platelet aggregation.…”

Section: Fig 6 Diverse Applications In Nanotechnologymentioning

confidence: 99%

“…where most of the citations show that nanoparticles can induce platelet aggregation. What makes a nanoparticle pro-aggregarory (Geys et al, 2008;Oberdörster et al, 2007;Deb et al, 2007Deb et al, ,2011Wiwanitkit et al, 2009;Radomski et al, 2005;Mayer et al, 2009;McGuinnes et al, 2010;Nemmar et al, 2003;Cejas et al, 2007;Wilson et al, 2010;Rückerl et al, 2007;Miller et al, 2009), inert (Li et al, 2009;Ramtoola et al, 2010;Gulati et al, 2010) or even anti-platelet in nature (Shrivastava et al, 2009;Koziara et al, 2005;Miller et al, 2009) is of great importance in development of ACS based nano-drugs, risk assessment in ACS , and also in evaluating resistance to ACS related drugs Jogns et al, 2006;Michelson et al, 2006). …”

Section: Fig 6 Diverse Applications In Nanotechnologymentioning

confidence: 99%