2014
DOI: 10.1590/1677-3225v13n2a01
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Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA

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Cited by 3 publications
(9 citation statements)
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(24 reference statements)
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“…The blood was allowed to clot over the specimens for 20 minutes in a humidified chamber at 37°C. The specimens were finally rinsed in phosphate-buffered saline (3 times×5 minutes each) in multi-well dishes with gentle rotating movements [ 4 ]. After rinsing, the specimens were fixed with 2.5% glutaraldehyde in 0.15 M cacodylate buffer solution (pH 7.2) for 4 hours, rinsed in 0.15 M cacodylate buffer, and dehydrated in ascending ethanol series solutions (50%, 70%, 90%, 95%, 100%; 5 minutes each concentration) [ 13 ].…”
Section: Methodsmentioning
confidence: 99%
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“…The blood was allowed to clot over the specimens for 20 minutes in a humidified chamber at 37°C. The specimens were finally rinsed in phosphate-buffered saline (3 times×5 minutes each) in multi-well dishes with gentle rotating movements [ 4 ]. After rinsing, the specimens were fixed with 2.5% glutaraldehyde in 0.15 M cacodylate buffer solution (pH 7.2) for 4 hours, rinsed in 0.15 M cacodylate buffer, and dehydrated in ascending ethanol series solutions (50%, 70%, 90%, 95%, 100%; 5 minutes each concentration) [ 13 ].…”
Section: Methodsmentioning
confidence: 99%
“…Obtaining clean, smooth, and decontaminated root surfaces during periodontal surgery is imperative for the organization of new connective tissue re-attachment and enhancement of the tissue healing process [ 1 ]. Even though ultrasonic or manual scaling and root planing are the most widely used techniques in clinical settings for the mechanical cleaning of the root surface during periodontal therapy [ 2 3 ], they cannot fully decontaminate the dental hard tissue [ 4 ]. Moreover, due to mechanical debridement, a compact smear layer is formed in intimate contact with the treated surface, constituted by a mixture of organic and inorganic materials, microbial toxins, and debris [ 4 5 ].…”
Section: Introductionmentioning
confidence: 99%
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