Blood Cell Biomechanics Evaluated by the Single-Cell Micromanipulation

Lelièvre, Jean-Claude; Bucherer, C.; Geiger, S.; Lacombe, C.; Vereycken, V.

doi:10.1051/jp3:1995218

Cited by 17 publications

(20 citation statements)

References 21 publications

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“…We note that our measured values for K A for the healthy RBCs are in agreement with recent studies based on measuring the dynamic membrane fluctuation which reveal that K A is in the range of 1–10 μNm −1 [22,23]. However, these values for K A for the healthy RBCs are lower than the values reported from micropipette aspiration studies which find K A to be in the range of 300–500 mN m −1 [21,24,25]. This large discrepancy between the micropipette-based technique and the measurement of dynamic membrane fluctuation can be rationalized by the fact that the former probes behavior dominated by the lipid bilayer and the latter deals with behavior dominated by the spectrin network.…”

Section: Methodssupporting

confidence: 90%

“…However, these values for K A for the healthy RBCs are lower than the values reported from micropipette aspiration studies, i.e. in the range of 300– 500 mN m −1 [21,24,25]. As discussed earlier in Section 2.5 in detail, this discrepancy between the micropipette-based method and the membrane fluctuation based technique can be attributed by the fact that the former tends to probe the lipid bilayer and the latter the spectrin network.…”

Section: Discussionmentioning

confidence: 76%

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Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient

et al. 2012

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Section: Methodssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 76%

Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient

et al. 2012

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“…These values are summarized in Table 1. Accordingly, we derived the shear modulus (μ) and the area expansion modulus (dilation elasticity, κ) based on the following equations (30,31) under the condition of 1 < ΔL/R p < 5 (linear response region in Fig. 5B), respectively:…”

Section: Applied Physical Sciencesmentioning

confidence: 99%

“…In other words, the appearance of such a small pore on the DNA gel network might lead the droplet flow into the pipette above the critical length (ΔL c ). This phenomenon corresponds to the fact that the DNA shell has a small shear modulus (μ) and a large area expansion modulus (κ), i.e., κ/μ >> 1 (Table 1), which is similar to cells with cytoskeletons, such as red blood cells and white cells (30,31,38).…”

Section: Applied Physical Sciencesmentioning

confidence: 99%

DNA cytoskeleton for stabilizing artificial cells

Kurokawa

Fujiwara

Morita

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

127

123

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Cell-sized liposomes and droplets coated with lipid layers have been used as platforms for understanding live cells, constructing artificial cells, and implementing functional biomedical tools such as biosensing platforms and drug delivery systems. However, these systems are very fragile, which results from the absence of cytoskeletons in these systems. Here, we construct an artificial cytoskeleton using DNA nanostructures. The designed DNA oligomers form a Y-shaped nanostructure and connect to each other with their complementary sticky ends to form networks. To undercoat lipid membranes with this DNA network, we used cationic lipids that attract negatively charged DNA. By encapsulating the DNA into the droplets, we successfully created a DNA shell underneath the membrane. The DNA shells increased interfacial tension, elastic modulus, and shear modulus of the droplet surface, consequently stabilizing the lipid droplets. Such drastic changes in stability were detected only when the DNA shell was in the gel phase. Furthermore, we demonstrate that liposomes with the DNA gel shell are substantially tolerant against outer osmotic shock. These results clearly show the DNA gel shell is a stabilizer of the lipid membrane akin to the cytoskeleton in live cells.iposomes have been used as artificial cell models to understand cell shape, membrane protein function, and lipid− protein interaction, among other biological functions (1-3). In addition, liposomes have been used as a platform for biosensing and as drug delivery systems (DDS) because of their excellent biocompatibility and biodegradability (4). However, liposomes collapse easily against environmental shifts and mechanical forces because of their low bending modulus. The fragility of liposomes causes uncontrolled leakage of the entrapped compounds and thus inhibits their use in biomedical applications and artificial cells experiments.In contrast, cell membranes are tolerant against environmental shifts and mechanical forces. The stability of cell membrane arises from the cytoskeleton underneath the membrane. The major component of cytoskeletons is actin (5). Actin gels show high elasticity (6), which ensures the stability of cell membranes against various forces. For liposomes, the use of actin filaments as a cytoskeleton is not an optimal strategy for the following three reasons: First, although actin bundles and actomyosin rings have been reconstituted in artificial cells (7,8), formation of an actin cortex underneath artificial membranes has been still challenging. Second, actin is hard to modify by chemical and genetic means because of its essentiality for cell growth. Third, the physicochemical properties of actin gels are still unclear (9, 10). Hence, the cytoskeleton of liposomes should be constructed with defined and designable materials. To accomplish this aim, DNA nanotechnology, which uses limited components with high designability in a nanometer scale (11), is a feasible candidate to construct cytoskeleton structures in artificial cells.DNA nanostructure...

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“…We also obtain the same scaled standard deviation from the study of Evans et al [26] who found G s = 9 ± 1.7. Lelièvre et al [37] found G s = 4.5 ± 0.8 and thus their scaled standard deviation is 0.44.…”

Section: Nature and Shear Modulus Of The Erythrocyte Membranementioning

confidence: 99%

Analysis of the variation in the determination of the shear modulus of the erythrocyte membrane: Effects of the constitutive law and membrane modeling

Dimitrakopoulos

2012

Phys. Rev. E

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Despite research spanning several decades, the exact value of the shear modulus Gs of the erythrocyte membrane is still ambiguous, and a wealth of studies, using measurements based on micropipette aspirations, ektacytometry systems and other flow chambers, and optical tweezers as well as application of several models have found different average values in the range 2–10 µN/m. Our study shows that different methodologies have predicted the correct shear modulus for the specific membrane modeling employed, i.e. the variation in the shear modulus determination results from the specific membrane modeling. Available experimental findings from ektacytometry systems and optical tweezers suggest that the dynamics of the erythrocyte membrane is strain-hardening at both moderate and large deformations. Thus the erythrocyte shear modulus cannot be determined accurately using strain-softening models (such as the neo-Hookean and Evans laws) or strain-softening/strain-hardening models (such as the Yeoh law) which overestimate the erythrocyte shear modulus. According to our analysis, the only available strain-hardening constitutive law, the Skalak et al. law, is able to match well both deformation-shear rate data from ektacytometry and force-extension data from optical tweezers at moderate and large strains, using an average value of the shear modulus of Gs = 2.4–2.75 µN/m, i.e. very close to that found in the linear regime of deformations via force-extension data from optical tweezers, Gs = 2.5±0.4 µN/m. In addition, our analysis suggests that a standard deviation in Gs of 0.4–0.5 µN/m (owing to the inherent differences between erythrocytes within a large population) describes well the findings from optical tweezers at small and large strains as well as from micro-pipette aspirations.

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Blood Cell Biomechanics Evaluated by the Single-Cell Micromanipulation

Cited by 17 publications

References 21 publications

Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient

Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient

DNA cytoskeleton for stabilizing artificial cells

Analysis of the variation in the determination of the shear modulus of the erythrocyte membrane: Effects of the constitutive law and membrane modeling

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