Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient

Byun, HeeSu; Hillman, Timothy R.; Higgins, John M.; Diez-Silva, Monica; Peng, Zhangli; Dao, Ming; Dasari, Ramachandra R.; Suresh, S.; Park, YongKeun

doi:10.1016/j.actbio.2012.07.011

Cited by 114 publications

(136 citation statements)

References 58 publications

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“…In the sickle RBCs, their equivalent shear modulus is significantly higher than that for the healthy RBC [14,30,31]. In this study, we modelled a healthy RBC using the MS-RBC model with the following parameters: [23], the effective shear modulus of the sickle RBC was first chosen to be 2000 times larger than the value of the healthy RBC in the current study.…”

Section: Resultsmentioning

confidence: 99%

“…Actually, two effects appear to counteract each other: on the one hand, HU treatment is associated with an elevated mean corpuscular volume (MCV) and an increased Hct, resulting in a higher blood viscosity [9]. On the other hand, HU treatment is also associated with improved cell hydration [12] and cell deformability [13,14], and consequently a lower blood viscosity [10]. Interestingly, sickle RBCs after HU treatment always exhibit higher RBC deformability and higher MCV, which raises the question: To what extent does the HU treatment enhance RBC performance in microcirculation, thereby improving oxygen delivery in the body?…”

Section: Introductionmentioning

confidence: 99%

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Patient-specific blood rheology in sickle-cell anaemia

Lei

et al. 2016

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Sickle-cell anaemia (SCA) is an inherited blood disorder exhibiting heterogeneous cell morphology and abnormal rheology, especially under hypoxic conditions. By using a multiscale red blood cell (RBC) model with parameters derived from patient-specific data, we present a mesoscopic computational study of the haemodynamic and rheological characteristics of blood from SCA patients with hydroxyurea (HU) treatment (on-HU) and those without HU treatment (off-HU). We determine the shear viscosity of blood in health as well as in different states of disease. Our results suggest that treatment with HU improves or worsens the rheological characteristics of blood in SCA depending on the degree of hypoxia. However, on-HU groups always have higher levels of haematocrit-to-viscosity ratio (HVR) than off-HU groups, indicating that HU can indeed improve the oxygen transport potential of blood. Our patient-specific computational simulations suggest that the HVR level, rather than the shear viscosity of sickle RBC suspensions, may be a more reliable indicator in assessing the response to HU treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Patient-specific blood rheology in sickle-cell anaemia

Lei

et al. 2016

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“…[18], [20], [24], and [25] with permission. 적혈구뿐만 아니라 말라리아(malaria) [18] 나 바베시아(babesia) [19] 같은 기생충 감염성 질환부터 겸상적혈구 빈혈증(sickle cell disease) [20] [27]…”

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Trends in Biophysical Studies Using Holographic Microscopy

Lee¹,

Kim²,

Park³

2016

Phys. High Tech.

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“…Optical imaging is a burgeoning area whereby various non-invasive techniques are employed explicitly to retrieve quantitative morphological (volume, surface area and sphericity), biochemical (haemoglobin content and concentration), and mechanical properties (dynamic membrane fluctuations) of individual bio-specimens specially RBCs which are key to diagnose the pathophysiology of a number of diseases in hematology [1][2][3][4]. Automated blood cell counters based on the complete blood count have been most commonly used to analyze the primary and critical properties of RBCs in clinical hematology but limitations exist in accurate diagnosis [5].…”

Section: Introductionmentioning

confidence: 99%

Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging

Kumar

Srivastava

Mehta

et al. 2016

Journal of the Optical Society of Korea

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Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationary in interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innate morphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laser beam is an alternative modality to overcome contact-induced perturbation but at the same time a steeply focused laser beam might cause photo-damage. In order to address both the photo-damage and substrate adherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beam of 'arbitrary intensity profile' generated by a source having cavity imperfections per se. Thus the immobilized RBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robust Michelson interferometer which was designed from a cubic beam splitter having one surface coated with reflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferograms from bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limited to work in reflection configuration only because of the availability of an upright microscope. Due to RBC's membrane being poorly reflective for visible wavelengths, quantitative information in the signal is weak and therefore, the quality of experimental results is limited in comparison to results obtained in transmission mode by various holographic techniques reported elsewhere.

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Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient

Cited by 114 publications

References 58 publications

Patient-specific blood rheology in sickle-cell anaemia

Patient-specific blood rheology in sickle-cell anaemia

Trends in Biophysical Studies Using Holographic Microscopy

Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging

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