Blocking tumor necrosis factor paved the way for targeted therapeutics in inflammatory diseases

Chu, Cong‐Qiu

doi:10.1097/cm9.0000000000001846

Cited by 3 publications

(3 citation statements)

References 51 publications

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“…In this study, the TNF-α concentration was also measured in our study and showed no change in the rats with a neoplastic process (20.26 ± 3.79 pg/mL) as compared with the TNF-αRMK1 is an inflammatory cytokine produced during acute inflammation and is responsible for a diverse range of signaling events within cells, leading to necrosis or apoptosis [36][37][38].…”

Section: Resultsmentioning

confidence: 51%

“…Blocking TNF-α activity was discovered to reduce the toxicity and may enhance the therapeutic effect of immune checkpoint inhibitors [ 42 , 43 , 44 ]. At the same time, the cytokine was found to show cancer-promoting effects [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

“…PMAA normalized the TGF-β1 concentration to 303.76 ± 49.74 pg/mL (Figure 4). TNF-αRMK1 is an inflammatory cytokine produced during acute inflammation and is responsible for a diverse range of signaling events within cells, leading to necrosis or apoptosis [36][37][38].…”

Section: Resultsmentioning

confidence: 99%

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Effect of Poly(methacrylic acid) on the Cytokine Level in an In Vivo Tumor Model

et al. 2022

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Cancer is a leading cause of mortality globally. Despite remarkable improvements in cancer-treatment approaches, disease recurrence and progression remain major obstacles to therapy. While chemotherapy is still a first-line treatment for a variety of cancers, the focus has shifted to the development and application of new approaches to therapy. Nevertheless, the relationship between immune response, neoplastic diseases and treatment efficiency is not fully understood. Therefore, the aim of the study was to investigate the immunopharmacological effects of methacrylic acid homopolymer in an in vivo tumor model. Materials and methods: Monomeric methacrylic acid was used to synthesize polymers. Methacrylic acid was polymerized in dioxane in the presence of 4-Cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl]pentanoic acid. To study the molecular weight characteristics of PMAA by GPC, carboxyl groups were preliminarily methylated with diazomethane. An experimental cancer model was obtained by grafting RMK1 breast cancer cells. The serum levels of IL-6, IL-10, IL-17, transforming growth factor β1 (TGF-β1), and tumor necrosis factor α (TNF-α) were measured by ELISA. Results: The effect of PMAA on the serum concentrations of several cytokines was studied upon its single administration to laboratory animals in early neoplastic process. The IL-6, IL-17 and TGF-β1 concentrations were found to change significantly and reach the level observed in intact rats. The IL-10 concentration tended to normalize. Conclusion: The positive results obtained are the basis for further studies on the effect of methacrylic-acid polymers with different molecular-weight characteristics on the neoplastic process.

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Section: Resultsmentioning

confidence: 51%

Section: Resultsmentioning

confidence: 99%

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Effect of Poly(methacrylic acid) on the Cytokine Level in an In Vivo Tumor Model

et al. 2022

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A systematic review and meta-analysis of the stability of peripheral immune markers in healthy adults

Walsh

Lindsay

Grosse

et al. 2023

Brain, Behavior, and Immunity

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Differential Effects of Somatostatin on TNF Receptors and Apoptosis in Hepatocellular Carcinoma Cell Lines

Georgiadou,

Notas,

Tsomidis

et al. 2024

Gastroenterology Insights

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The anti-tumoral activity of somatostatin has been demonstrated in both animal experiments and human tumors. Clinical trials have reported conflicting results. We therefore hypothesized that somatostatin might have different effects in various hepatocellular carcinoma cells. Their clarification would possibly allow for the better selection of patients suitable for the optimal treatment results. We studied the mRNA and protein expression of TNF receptors and the TNFa-induced apoptosis using the HepG2 and the Hep3B human hepatocellular carcinoma cells after incubation with the somatostatin analog octreotide. RT-PCR, Western blot, and parameters associated with apoptosis (NF-kB nuclear translocation, P65 Ser536 and P65 Ser468 phosphorylation, DNA fragmentation) were assessed. Only TNFR1 was constitutively present in the two cell lines. Octreotide incubation led to an earlier reduction in TNFR1 mRNA and protein in HepG2 compared to Hep3B cells (1 h and 6–12 h, respectively). NF-kB translocation to the nucleus was induced by TNFa and was more prominent in Hep3B. Translocation was unaffected by octreotide. Serine phosphorylation was significantly induced by TNFa and was more evident in the Hep3B cells. TNFa-induced Ser536 phosphorylation was inhibited by octreotide only in the HepG2 cells. DNA fragmentation was not influenced by either octreotide or TNFa in the HepG2 cells, but TNFa induced fragmentation in the Hep3B cells (1.8-fold increase) verified by the TUNEL index (43 compared to 19 for the HepG2 cells). Octreotide and TNFa co-incubation induced apoptosis in the HepG2 cells (1.7-fold increase compared to controls) but inhibited apoptosis in the Hep3B cells. We conclude that: (1) octreotide reduced TNFR1 receptor expression in both cell lines, (2) parameters of apoptosis were differentially affected by octreotide in the two cell lines, and (3) this might be a partial explanation for the conflicting results of somatostatin analog treatment in human hepatocellular carcinoma trials.

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Blocking tumor necrosis factor paved the way for targeted therapeutics in inflammatory diseases

Cited by 3 publications

References 51 publications

Effect of Poly(methacrylic acid) on the Cytokine Level in an In Vivo Tumor Model

Effect of Poly(methacrylic acid) on the Cytokine Level in an In Vivo Tumor Model

A systematic review and meta-analysis of the stability of peripheral immune markers in healthy adults

Differential Effects of Somatostatin on TNF Receptors and Apoptosis in Hepatocellular Carcinoma Cell Lines

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