Block copolymers of Pluronics and poly-(2-dimethylamino+ethyl methacrylate) for delivery of oligonucleotides into tumor cells

Valeeva, Y.K.; Dorodnykh, T. Yu.; Alexandrova, N.A.; Zubin, E. M.; Kachalova, Anna V.; Волков, Е. М.; Gottikh, Marina; Melik‐Nubarov, N. S.

doi:10.1016/s1773-2247(06)50046-2

Cited by 8 publications

(6 citation statements)

References 42 publications

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“…In Fig. 12 we show the orientational order parameter, q, as a function of the projected distance | d| to the center of mass of the chain, where the tensor-order parameter, Qa,b , has been averaged with respect to separate bins in | d| according to eqn (4). In an interval of | d| with the largest enhancement of solvent permeability, 2 ( | d| ( 10 (see Fig.…”

Section: Bilayer Perturbations and Solvent Permeabilitymentioning

confidence: 99%

“…On the other hand, amphiphilic polymer structures such as block copolymers with hydrophilic/hydrophobic blocks 4,5 as well as polymers with random distribution of amphiphilic sites such as polyelectrolytes 6,7 and polyacrylates 2 may destabilize bilayer membranes and enhance passive transport of other molecules such as DNA strands, 8 oligonucleotides 4,9 or salt. 7 Destabilization effects can be observed as leakage of vesicles, 2 hemolysis of red blood cells 10,11 and increased rates of lipid flip-flops.…”

Section: Introductionmentioning

confidence: 99%

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Homo-polymers with balanced hydrophobicity translocate through lipid bilayers and enhance local solvent permeability

2012

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Section: Bilayer Perturbations and Solvent Permeabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Homo-polymers with balanced hydrophobicity translocate through lipid bilayers and enhance local solvent permeability

2012

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“…Cationic polymers bearing amino functional groups are an important class of materials capable of serving as non-viral carriers for DNA delivery to living cells, and have been previously employed for various gene transfection applications. [1][2][3][4][5] Such polymers include poly(ethyleneimine), 6 poly(4-hydroxy-Lproline ester) (PHP), 7,8 poly[a-(4-aminobutyl)-L-glycolic acid] (PAGA), 9,10 poly(b-amino esters) (PBAE), 11,12 poly(amine-coesters), 13 poly(L-lysine), 14 chitosan, 15 poly(dimethylaminoethyl methacrylate), 16 and poly(trimethylaminoethyl methacrylate). 17 Among these materials, polyesters containing tertiary amino substituents, such as poly(amine-co-esters) and PBAEs, are particularly attractive due to their biodegradability, low cytotoxicity, and outstanding gene transfection efficacy.…”

Section: Introductionmentioning

confidence: 99%

Exploring the solid state properties of enzymatic poly(amine-co-ester) terpolymers to expand their applications in gene transfection

et al. 2014

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Polymers bearing amino functional groups are an important class of materials capable of serving as non-viral carriers for DNA delivery to living cells. In this work biodegradable poly(amine-co-ester) terpolymers were synthesized via ring-opening and polycondensation copolymerization of lactone (ε-caprolactone (CL), ω-dodecalactone, ω-pentadecalactone (PDL), and ω-hexadecalactone) with diethyl sebacate (DES) and N-methyldiethanolamine (MDEA) in diphenyl ether, catalyzed by Candida antarctica lipase B (CALB). All lactone-DES-MDEA terpolymers had random distributions of lactone, sebacate, MDEA repeat units in the polymer chains. PDL-DES-MDEA terpolymers were studied in the composition range from 21 mol% to 90 mol% PDL whereas the terpolymers with other lactones were investigated at a single composition (80 mol% lactone). DSC and WAXS analyses showed that all investigated terpolymers crystallize in their respective homopolylactone crystal lattice. Terpolymers with large lactones and a high lactone content melt well above room temperature and are hard solids, whereas terpolymers with small lactones (e.g. CL) or with a low lactone content melt below/around ambient temperature and are waxy/gluey materials. Given the importance of hydrophobicity in influencing gene delivery, water contact angle measurements were carried out on lactone-DES-MDEA terpolymers showing that it is possible to tune the hydrophilic-to-hydrophobic balance by varying polymer composition and size of lactone units. To demonstrate the feasibility of using solid terpolymers as nanocarriers for DNA delivery, PDL-DES-MDEA copolymers with 65–90% PDL were successfully transformed into free-standing nanoparticles with average particle size ranging from 163 to 175 nm. Our preliminary results showed that LucDNA-loaded nanoparticles of the terpolymer with 65% PDL were effective for luciferase gene transfection of HEK293 cells.

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“…They can self-assemble into micelles with a hydrophobic core by PPO and a hydrophilic shell by PEO. 17 The pluronic copolymers have been modified with cationic polymers, such as PEI, 18 PLL, 19 and PDMAEMA, [20][21][22] to improve gene transfection as well as to reduce cytotoxicity. The hydrophobic PPO segments of pluronic polymers enhance the stability of polyplexes between polymers and deoxyribonucleic acid (DNA), 23,24 and promote the cellular uptake of polyplexes because of the increased interaction between the cell membrane and polyplexes.…”

Section: Introductionmentioning

confidence: 99%

Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies

Huang

Wang²,

Lue³

et al. 2013

IJN

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Cationic polymers are one of the major nonviral gene delivery vectors investigated in the past decade. In this study, we synthesized several cationic copolymers using atom transfer radical polymerization (ATRP) for gene delivery vectors: pluronic F127-poly(dimethylaminoethyl methacrylate) (PF127-pDMAEMA), pluronic F127-poly (dimethylaminoethyl methacrylatetert-butyl acrylate) (PF127-p(DMAEMA-tBA)), and pluronic F127-poly(dimethylaminoethyl methacrylate-acrylic acid) (PF127-p(DMAEMA-AA)). The copolymers showed high buffering capacity and efficiently complexed with plasmid deoxyribonucleic acid (pDNA) to form nanoparticles 80-180 nm in diameter and with positive zeta potentials. In the absence of 10% fetal bovine serum, PF127-p(DMAEMA-AA) showed the highest gene expression and the lowest cytotoxicity in 293T cells. After acrylic acid groups had been linked with a fluorescent dye, the confocal laser scanning microscopic image showed that PF127-p(DMAEMA-AA)/pDNA could efficiently enter the cells. Both clathrin-mediated and caveolae-mediated endocytosis mechanisms were involved. Our results showed that PF127-p(DMAEMA-AA) has great potential to be a gene delivery vector.

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Block copolymers of Pluronics and poly-(2-dimethylamino+ethyl methacrylate) for delivery of oligonucleotides into tumor cells

Cited by 8 publications

References 42 publications

Homo-polymers with balanced hydrophobicity translocate through lipid bilayers and enhance local solvent permeability

Homo-polymers with balanced hydrophobicity translocate through lipid bilayers and enhance local solvent permeability

Exploring the solid state properties of enzymatic poly(amine-co-ester) terpolymers to expand their applications in gene transfection

Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies

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