Homo-polymers with balanced hydrophobicity translocate through lipid bilayers and enhance local solvent permeability

Werner, Marco; Sommer, Jens‐Uwe; Baulin, Vladimir A.

doi:10.1039/c2sm26008e

Cited by 46 publications

(99 citation statements)

References 40 publications

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“…The mechanism of direct penetration by an amphipathic polymer was confirmed using Monte Carlo simulations . The results indicate that appropriate adjustment of the hydrophobicity of a polymer chain makes the fluctuating lipid bilayer transparent; namely, the relative hydrophobicities of the polymers are balanced so that both the solvent and the hydrophobic core in the membrane are equally poor environments for the chain.…”

Section: Direct Penetration Across Plasma Membranementioning

confidence: 82%

Critical update on 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer science

Goda

Ishihara

Miyahara

2015

J of Applied Polymer Sci

119

105

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2-Methacryloyloxyethyl phosphorylcholine (MPC) is a custom methacrylate with a zwitterionic phosphorylcholine moiety on the side chain. In the past 25 years, MPC has been used as a building block for a wide range of polymeric biomaterials because of its excellent resistance to nonspecific protein adsorption, cell adhesion, and blood coagulation. Recently, MPC polymers with specific features have been used in bioengineering and nanomedicine. This review focuses on three topics that highlight the latest findings on MPC polymers, that is, specific recognition of C-reactive protein (CRP), cell-membrane-penetration abilities, and lubrication properties. These developments will extend the applications of this biomimetic material from bioinert polymers to biosensing, CRP inhibitors, prodrug carriers, subcellular bioimaging, cell manipulation, and joint replacement.

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Section: Direct Penetration Across Plasma Membranementioning

confidence: 82%

Critical update on 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer science

Goda

Ishihara

Miyahara

2015

J of Applied Polymer Sci

119

105

View full text Add to dashboard Cite

show abstract

“…Baulin and co-workers investigated the translocation behavior of amphiphilic polymers through lipid bilayers using MDS [39]. According to the simulations, fully hydrophilic polymer formed random coils in solution and was rejected from the bilayer while completely hydrophobic polymer turned into a globular structure, trapped inside the hydrophobic core of the bilayer in a quasi-two-dimensional solvent of tails.…”

Section: Mechanisms Of Actionmentioning

confidence: 99%

Controlling macromolecular structures towards effective antimicrobial polymers

Ganewatta

Tang

2015

Polymer

219

255

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“…In contrast, computer simulations can capture such events with molecular or higher resolution. Translocation across phospholipid membranes has already been investigated for many small molecules (14)(15)(16), polymers (17), and nanoparticles (18). Nanoparticles are typically much larger than peptides, and their membrane crossing is associated with the formation of a large membrane pore or defect (19).…”

Section: Introductionmentioning

confidence: 99%

“…Polymers of a size comparable to peptides can be less disruptive than large nanoparticles and can translocate without inducing large membrane defect. Indeed, coarse-grained simulations showed that polymers with optimal hydrophobicity could cross the membrane without large free-energy changes (17). Such polymers change their conformation from expanded to compact upon membrane insertion and remain unstructured.…”

Section: Introductionmentioning

confidence: 99%

Optimal Hydrophobicity and Reorientation of Amphiphilic Peptides Translocating through Membrane

Kabelka

Vácha

2018

Biophysical Journal

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Cell-penetrating and some antimicrobial peptides can translocate across lipid bilayers without disrupting the membrane structure. However, the molecular properties required for efficient translocation are not fully understood. We employed the Metropolis Monte Carlo method together with coarse-grained models to systematically investigate free-energy landscapes associated with the translocation of secondary amphiphilic peptides. We studied α-helical peptides with different length, amphiphilicity, and distribution of hydrophobic content and found a common translocation path consisting of adsorption, tilting, and insertion. In the adsorbed state, the peptides are parallel to the membrane plane, whereas, in the inserted state, the peptides are perpendicular to the membrane. Our simulations demonstrate that, for all tested peptides, there is an optimal ratio of hydrophilic/hydrophobic content at which the peptides cross the membrane the easiest. Moreover, we show that the hydrophobicity of peptide termini has an important effect on the translocation barrier. These results provide general guidance to optimize peptides for use as carriers of molecular cargos or as therapeutics themselves.

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Homo-polymers with balanced hydrophobicity translocate through lipid bilayers and enhance local solvent permeability

Cited by 46 publications

References 40 publications

Critical update on 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer science

Critical update on 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer science

Controlling macromolecular structures towards effective antimicrobial polymers

Optimal Hydrophobicity and Reorientation of Amphiphilic Peptides Translocating through Membrane

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