Blending Bacillus sp., Lysinibacillus sp. and Rhodococcus sp. for optimal reduction of heavy metals in leachate contaminated soil

Emenike, C. U.; Agamuthu, P.; Fauziah, Shahul Hamid

doi:10.1007/s12665-015-4805-9

Cited by 46 publications

(26 citation statements)

References 22 publications

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“…Soil samples were excavated at 30 cm depth in accordance with the 2014 ASTME-1197 [6], [7]. The samples were analyzed for pH using a multiprobe meter (YSI Professional Plus, USA), while the soil total nitrogen, total potassium, and total phosphorus were analyzed by adopting ASTM E778-87, ASTM E96-94, and ASTM D5198-92 methods, respectively.…”

Section: Soil and Leachate Characterizationmentioning

confidence: 99%

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Remediation of Heavy Metal Contaminated Soil Using Potential Microbes Isolated from a Closed Disposal Site

Fauziah¹,

Barasarathi²,

Emenike³

et al. 2017

IJBBB

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Soil environment is a major sink for a multitude of chemicals and heavy metals, which inevitably leads to environmental contamination problems. Various human activities including agricultural, urban or industrial, or landfilling are major contributors to heavy metal contamination in the environment. Since landfilling is one of the ultimate waste disposal methods, the generation of leachate is inevitable. Leachate from landfill is highly heterogeneous and consist high amount of heavy metal. Subsequent movement of the leachate into the surrounding soil, ground water or surface water could lead to severe pollution problems to and cause toxicity to human and other living organisms. Microorganisms has the ability to solubilize the metals (or increase their bioavailability) via the production of siderophores and adsorb the metals in their biomass on metal-induced outer membrane proteins and by bio precipitation. Therefore this study aimed to remediate heavy metal in leachate contaminated soil from a closed non-sanitary landfill in Kuala Lumpur. Preliminary soil and leachate characterization revealed high amount of metal contaminants as compared to the prescribed limit by local and international standard. Total of eighteen microbes were isolated from the contaminated site and were grouped into two treatments, proteobacteria and non-proteo bacteria.Comparison between the treatments revealed that proteobacteria (Treatment A) were performing higher metal removal activity compared to non-proteobacteria (Treatment B) and control (Treatment C). Out of four metals tested in this study, three of the metals (As (71.86%), Ni (50.8%), Al (87.15%)) were removed significantly by the addition of Treatment A. Highest metal removal rate constant was obtained for Al at 0.02 day -1 . Therefore, it can be concluded that the addition of microbes, namely proteobacteria to leachate contaminated soil can remove the heavy metal content at a significant rate.

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Section: Soil and Leachate Characterizationmentioning

confidence: 99%

“…In an effective bioremediation process, microorganisms will enzymatically attack the pollutants and convert them to harmless products through chemical, physical and biological [6].…”

Section: Introductionmentioning

confidence: 99%

Remediation of Heavy Metal Contaminated Soil Using Potential Microbes Isolated from a Closed Disposal Site

Fauziah¹,

Barasarathi²,

Emenike³

et al. 2017

IJBBB

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“…At this point Cr reduction was the highest (81%) after 100 days of monitoring. It is possible that the metal binding effect of Lysinibacillus sp was enhanced and better expressed when in association with Bacillus sp and Rhodococcus sp which are also gram-positive bacteria [2]. Mechanisms behind the enhanced result may also be associated with increase in microbial population within a polluted environment, but most significant is the manipulation of microbial diversity.…”

Section: International Journal Of Bioscience Biochemistry and Bioinfmentioning

confidence: 99%

“…Hence, this is the reason bioremediation of leachate-metal polluted soil is very important. A sustainable soil remediation approach is any method that involves technical removal of pollutants while soil quality and functioning are preserved [2], [3]. Basically, bioremediation is primarily the use of degrader microbes, but the toxic level of contaminants within a polluted soil environment may only allow the survival of some microbes rather than giving chance for pollutant degradation (in case of most organic compounds) or transformation (in case of most inorganic compounds like metals) [4].…”

Section: Introductionmentioning

confidence: 99%

“…Basically, bioremediation is primarily the use of degrader microbes, but the toxic level of contaminants within a polluted soil environment may only allow the survival of some microbes rather than giving chance for pollutant degradation (in case of most organic compounds) or transformation (in case of most inorganic compounds like metals) [4]. However, when microbes are properly engineered or enhanced with respect to population, biochemical properties and diversity, they tend to optimally metabolize pollutants; metals inclusive [2]. Some bacteria species possess remediation potentials for metal pollution [5], [6].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Mixing Individual Isolates for Bioreduction of Metals in Contaminated Soil

Emenike¹,

Izyani²,

Fauziah³

2017

IJBBB

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Bioremediation of metal contaminated soil involves complex processes, and the non-degradable nature of metals makes it more difficult. The known sources of metal pollution are many and adopting a specific remediation procedure for it is often impossible. Microbes found at polluted sites tend to serve as good remediation agents due to perceived environmental potential. Therefore, it is imperative to select bioremediation by enhancing microbes potential as a way of restoring metal polluted site to original or undisturbed state. Hence, this study tried to evaluate the individual effect of bacterial isolates on the remediation of metal polluted soil from landfill, against combined impact of microbes blended together. Soil samples that characterized of Cd 2+ , Cr 2+ , Pb 2+ and Zn 2+ were subjected to bioremediation. Results revealed no more than 50% reduction across the treatments amended with individual isolates. Rather higher metal remediation was observed when individual isolates were mixed prior to application. Therefore, the study suggests that individual isolates from contaminated sites can selectively metabolize to bioreduce metal concentrations. However, a mix of such isolates with manipulation in the concentration level can enhance metal reduction efficiency (>50%).

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Optimal Removal of Heavy Metals From Leachate Contaminated Soil Using Bioaugmentation Process

Emenike

Liew

Fahmi

et al. 2017

CLEAN Soil Air Water

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Bioremediation of soil contaminated with metals from leachate is a complex process because it requires understanding the microbial interaction common to such contaminated environment. Therefore, this study tried to enhance the reduction of some extractable metals such as Pb, Cu, and Al through bioaugmentation with inocula of bacterial species isolated from leachate‐contaminated soil. Assessment of metal concentrations in the soil was carried out through characterization (US EPA 3050B test method). Furthermore, the persistent microbes were isolated from the contaminated soil before mixing the microbial culture in terms of combinations and concentration (1.3 ABS at 600 nm). The inoculum obtained was dispensed into the induced leachate‐contaminated garden soil (10% v/w). A combination of all the isolated microbes recorded less efficiency, unlike the selective use of only three bacterial species (Lysinibacillus sp., Bacillus sp., and Rhodococcus sp.) which gave better metal removal efficiency to a significant level (p < 0.05); extractable Pb, Al, and Cu were reduced by 71, 72, and 86%, respectively. This indicates that just amending the concentrations of microbes do not give the optimal remediation potential of the microbes, rather selection is very important. Lysinibacillus sp., Bacillus sp., and Rhodococcus sp. were the only isolated Gram‐positive bacteria that possibly influenced the metal removal efficiency when blended together as the bioaugmentation agent. The bioaugmentation process enhanced the reduction in concentrations of the heavy metals within the contaminated soil.

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Blending Bacillus sp., Lysinibacillus sp. and Rhodococcus sp. for optimal reduction of heavy metals in leachate contaminated soil

Cited by 46 publications

References 22 publications

Remediation of Heavy Metal Contaminated Soil Using Potential Microbes Isolated from a Closed Disposal Site

Remediation of Heavy Metal Contaminated Soil Using Potential Microbes Isolated from a Closed Disposal Site

Effect of Mixing Individual Isolates for Bioreduction of Metals in Contaminated Soil

Optimal Removal of Heavy Metals From Leachate Contaminated Soil Using Bioaugmentation Process

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