Bleach-concentrated direct sputum smear microscopy procedure for diagnosis of pulmonary tuberculosis in poverty areas

Abstract

The history of muscle biopsy dates back to 1860, when Duchenne first performed a biopsy on a patient with symptoms of myopathy (1) . Since then, the basic and clinical science of muscle and muscle disease has gone through three stages of development: the classical period, the modern stage and the molecular era.

Performance of Bleach Method Sputum Smear Microscopy for the Diagnosis of Tuberculosis in a Highly Endemic District in Lima, Peru

“…Additionally, we did not obtain interobserver comparison results between the microscopists who performed the SSM. Moreover, in our study, the first sputum sample was used to carry out the direct method, and the second for the bleach method, which contrasts with similar studies that carry out these treatments from a single sample [19,29,38,39]. The collection of sputum at different times could affect the results, if there were significant differences in AFB counts between the first and second sputum samples.…”